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Chrysanthemum-branching-trait-related molecular marker acquisition method

A technology of molecular markers and branching, which is applied in biochemical equipment and methods, microbiological determination/testing, DNA preparation, etc., can solve the problems that the methods for obtaining molecular markers associated with branching traits of chrysanthemums have not been reported, and achieve high repeatability , long cycle, high cost-efficiency effect

Inactive Publication Date: 2013-08-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, there are also reports on the molecular marker loci related to the ornamental traits of chrysanthemums, but the method of obtaining molecular markers associated with the branching traits of chrysanthemums has not been reported yet.

Method used

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  • Chrysanthemum-branching-trait-related molecular marker acquisition method
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  • Chrysanthemum-branching-trait-related molecular marker acquisition method

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Embodiment 1

[0046] (1) Acquisition of test materials and their phenotypic data

[0047] The test materials were the cut chrysanthemum varieties 'QX-145' and 'Nannong Yinshan' preserved in the "China Chrysanthemum Germplasm Resource Conservation Center" of Nanjing Agricultural University. The characters of some branches of the two varieties were significantly different. In the autumn of 2010, artificial hybridization was carried out with 'QX‐145' as the female parent and 'Nannong Yinshan' as the male parent. The well-developed flower buds of the female parent 'QX-145' were selected, castrated when the ligule-shaped flowers were just dew, and bagged with sulfuric acid paper bags, and the inflorescences of the male parent 'Nannong Yinshan' were bagged at the same time. When the stigma of the female parent protrudes and splits at an acute angle and secretes mucus, collect the fresh pollen of the bagged male parent, pollinate the female parent with a brush, bag it, and repeat the pollination ...

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Abstract

The invention relates to a chrysanthemum-branching-trait-related molecular marker acquisition method, and belongs to the technical field of biology. The method comprises the steps that: a, a testing material and phenotypic data thereof are obtained; b, a chrysanthemum linkage map is established; c, chrysanthemum branching trait QTL analysis is carried out according to the phenotypic data and a molecular genetic map; and d, chrysanthemum-branching-trait-related molecular markers are determined. According to the invention, 'QX-145' (P1) is adopted as a female parent, NanNongYinShan is adopted as a male parent (P2), and 92 F1 segregating populations obtained by hybridization are adopted as the testing material; and a plurality of molecular markers substantially related to chrysanthemum branching traits are obtained. The molecular markers can be used in chrysanthemum branching trait good gene fine positioning and cloning, such that selection efficiency can be greatly improved, and breeding process can be accelerated.

Description

technical field [0001] The invention discloses a method for obtaining molecular markers associated with branching traits of chrysanthemums, belongs to the field of biotechnology, and is used for positioning and cloning of excellent genes for branching traits of chrysanthemums and for cultivating new varieties of chrysanthemums. Background technique [0002] Chrysanthemum is one of my country's top ten traditional famous flowers and the world's four major cut flowers, and occupies an important position in flower production and landscaping. In recent years, there have been many studies on the inheritance of ornamental traits such as chrysanthemum plant type and flower organ, which has promoted the process of chrysanthemum hybrid breeding to a certain extent. However, with the continuous deepening of breeding work, the traditional hybrid breeding method has certain limitations in breeding because it takes a long time and cannot be targeted to improve specific traits. Molecular...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/68
Inventor 陈发棣彭辉房伟民蒋甲福陈素梅管志勇廖园
Owner NANJING AGRICULTURAL UNIVERSITY
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