Method for detecting quality of Bazhen particles

Abstract

The invention discloses a method for detecting quality of Bazhen particles, comprising the following steps of: using octadecylsilane chemically bonded silica as a filler; using acetonitrile-0.1% phosphoric acid solution as a mobile phase, wherein a detecting wavelength is 230nm and a flow velocity of the mobile phase is 0.8-1.2ml / min; precisely weighting a paeoniflorin reference substance, and adding 70% ethanol to prepare a paeoniflorin solution; precisely weighting the Bazhen particles, and placing the particles into an erlenmeyer flask with a plug, then precisely adding 50ml 70%ethanol, sealedly plugging the erlenmeyer flask, weighting the erlenmeyer flask, ultrasonically processing for 28-32min, cooling, then weighting, supplementing losing weight by the 70% ethanol, shaking up; taking 10mul each of the a reference substrate solution and a tested object solution, injecting it into a liquid chromatograph, and then detecting. The method of the invention has advantages of rapid and timely completion, and good stabilization and reappearance, can control product quality, can reduce body damage of working personnel, and can reduce unfavorable influence on working environment.

Description

Technical field: [0001] The invention belongs to the technical field of quality control of traditional Chinese medicine preparations, and in particular relates to a quality detection method of Bazhen granules. Background technique: [0002] Bazhen granule is recorded in the 2010 edition of "Chinese Pharmacopoeia". It is composed of Codonopsis pilosula, fried Atractylodes macrocephala, Poria cocos, roasted licorice, angelica, fried white peony root, Chuanxiong, and rehmannia glutinosa. Atractylodes macrocephala 60g, Poria cocos 60g, Zhigancao 30g, angelica 90g, fried white peony 60g, Chuanxiong 45g, Rehmannia glutinosa 90g and eight herbs. For the Bazhen granules, except for white peony root and licorice, the content of the main active ingredients in the medicinal materials has not been established, or the content of the active ingredients is so low that it is difficult or impossible to detect in the compound preparation; The acid content is relatively high, but after the fo...

AI Technical Summary

Problems solved by technology

At present, the above-mentioned quality control method in the prior art, wherein methanol is used as the mobile phase, because methanol is a very volatile liquid, the equipped mobile phase should be prepared and used in time, and cannot be placed for a long time. The configuration process is cumbersome. If you need to detect as soon as possible, you must configure it first. Therefore, it is not conducive to completing the detection quickly and in time.

Moreover, methanol is relatively toxic and easy to volatilize. Long-term exposure is not easy for the health of the staff.

In addition, triethylamine is a colorless and transparent liquid with a strong ammonia odor. It emits a little smoke in the air. Long-term exposure can easily cause harm to the human body and have a bad impact on the working environment.

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