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HER2 (Human Epidermal Growth Factor Receptor 2) protein nucleotide aptamer, complex, composition and applications thereof

A nucleic acid aptamer and complex technology, which is used in drug combinations, pharmaceutical formulations, recombinant DNA technology, etc.

Active Publication Date: 2015-04-29
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is of great significance and application value to develop an aptamer-based chemotherapeutic drug delivery system to target and kill HER2-positive tumor cells. Research Reports on Positive Tumor Cells

Method used

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  • HER2 (Human Epidermal Growth Factor Receptor 2) protein nucleotide aptamer, complex, composition and applications thereof
  • HER2 (Human Epidermal Growth Factor Receptor 2) protein nucleotide aptamer, complex, composition and applications thereof
  • HER2 (Human Epidermal Growth Factor Receptor 2) protein nucleotide aptamer, complex, composition and applications thereof

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Experimental program
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Embodiment 1

[0038] 1. Materials and methods

[0039] Reagent

[0040] Single-stranded oligonucleotides were synthesized by Invitrogen. Peptides were purchased from Beijing Saibaisheng Company. HER2 protein was purchased from Beijing Yiqiao Sino Biotechnology Co., Ltd., item number: 10004-H08H. Bovine serum albumin was purchased from Tianjin Haoyang Biotechnology Co., Ltd. Monodisperse magnetic beads and streptavidin-coated magnetic beads were purchased from Promega. EDC was purchased from Sigma Corporation.

[0041] cell line

[0042] Human breast cancer cells MCF7, MDA-MB-231, MDA-MB-453, SK-BR-3, and human normal liver cells L02 were purchased from the Cell Center of the Chinese Academy of Medical Sciences. The cells were cultured in DMEM high-glucose medium containing 10% FBS, regular concentrations of penicillin and streptomycin (manufacturer: Beijing Yingxin Sunshine Biotechnology Co., Ltd., item number: 07-021, trade name: DMEM high-glucose medium). cells at 37°C, 5% CO 2 cu...

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Abstract

The invention relates to a HER2 (Human Epidermal Growth Factor Receptor 2) protein nucleotide aptamer, a complex, a composition and applications thereof, and in particular relates to an HER2 (Human Epidermalgrowth Factor Receptor-2) protein specificity combined nucleotide aptamer which has the sequence shown in SEQ ID NO:5. The invention further relates to the complex formed by combining the HER2 protein specificity combined nucleotide aptamer with a chemotherapeutic medicine or wrapping the HER2 protein specificity combined nucleotide aptamer and the chemotherapeutic medicine in a nanoparticle together, the composition comprising the complex, and anti-tumor application as well as an tumor targeted imaging application of the nucleotide aptamer, the complex and the composition.

Description

technical field [0001] The present invention relates to HER2 protein nucleic acid aptamer, compound, composition and application thereof. Background technique [0002] Breast cancer is one of the common malignant tumors in women, and about 20% to 30% of the patients are human epidermal growth factor receptor-2 (ERBB2, HER2) positive (King CR, Kraus MH, Aaronson SA (1985) Amplification of a novel v-erbB-related gene in a human mammalian carcinoma. Science 229:974-976). Compared with other breast cancer patients, HER2-positive patients have higher tumor malignancy, faster progression, easier recurrence and distant metastasis, and poorer long-term survival rate (Garrett JT, Arteaga CL (2011) Resistance to HER2-directed Antibodies and tyrosine kinase inhibitors: mechanisms and clinical implications. Cancer Biol Ther 11: 793-800.; Nahta R, O'Regan RM (2010) Evolving strategies for overcoming resistance to HER2-directed therapy: targeting the PI3K / Akt / mTOR pathway .Clin Breast C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115A61K47/26A61K45/00A61K49/00A61K49/10A61P35/00
Inventor 杨先达刘喆
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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