Purity detection method for double-optimal 888 salt seeds of bilinear hybrid rice

Abstract

The invention discloses a purity detection method for double-optimal 888 salt seeds of bilinear hybrid rice. By the method, according to a theory that after years of research, the salt tolerance of the double-optimal 888 salt seeds of the bilinear hybrid rice and the salt tolerance of the female parent 582S salt seeds of the bilinear hybrid rice are obviously different; under a certain salt (Nacl) concentration stress condition, the double-optimal 888 salt seeds and the 582S salt seeds (contrast) are cultured in a laboratory to obtain young seedlings; after the seedlings appear until 3-4 leaf periods, and if leaf tips are curled, leaves are withered, and the leaves slowly grow, the seeds are intolerant against the salt, and the seeds are the double-optimal 888 salt seeds; under the salt (Nacl) stress condition, if the leaves normally grow, the seeds are tolerant against the salt, and the seeds are solid seeds obtained by selfing of the female parent 582S salt seeds and the double-optimal 888 salt seeds in the last season; and therefore, the purity of the double-optimal 888 salt seeds can be calculated. By the method, the purity of the double-optimal 888 salt seeds of the bilinear hybrid rice can be quickly detected and the cost and the time of performing field planting on hybrid rice seeds in Hainan are saved.

Description

Technical field [0001] The invention belongs to the technical field of seed quality detection, and relates to a method for detecting the purity of hybrid rice seeds, more specifically a method for detecting the purity of two-line hybrid rice Yanliangyou 888. Background technique [0002] Rice is my country's largest food crop. my country is the world's largest producer and consumer of hybrid rice seeds. The production of high-purity rice hybrids is the basis for fully exploiting the potential of heterosis to increase production. Recently, the research and promotion of two-line hybrid rice has made a positive contribution to rice production in my country. Since the female parent of the two-line hybrid rice is a photo-thermo-sensitive genic male sterile line, the photo-thermo-sensitive genic male sterile line has entered reproductive growth. The early stage is easily affected by environmental conditions such as light and temperature. When the environmental temperature is lower than ...

AI Technical Summary

Problems solved by technology

The off-site planting identification method is a more commonly used method, but because the off-site planting identification of hybrid rice seeds is often arranged in Hainan, it generally requires a rice growth cycle, that is, samples are sent to Hainan for planting in November every year, and the rice can be planted in April-May of the following year. Knowing the identification results, the identification results often lag behind the sales of seeds, which also causes seed quality disputes due to seed purity issues every year