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A gene regulating rice seed setting rate and its application

A technology of seed setting rate and coding genes, which is applied to genes regulating rice seed setting rate and its application fields, and can solve problems such as cloning and research without breakthroughs

Active Publication Date: 2016-05-25
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no breakthroughs have been made in the cloning and research of genes related to rice seed setting rate in this field

Method used

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  • A gene regulating rice seed setting rate and its application
  • A gene regulating rice seed setting rate and its application
  • A gene regulating rice seed setting rate and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0227] Preparation and detection of embodiment 1 rice mutant

[0228] This example was produced by transforming a plasmid carrying T-DNA and inserting it into the chromosome of rice through the commonly used transgenic experimental means.

[0229] The traits of rice mutants: the main traits of rice mutants are the two traits of seed setting rate and thousand-grain weight. The seed setting rate is the percentage of full grains to the total grains (full grains + empty grains) after the rice matures. Thousand-grain weight is the weight of one thousand seeds expressed in grams. It is an indicator of the size and fullness of the seeds, a test of seed quality and crop test content, and an important basis for predicting yield in the field.

Embodiment 2

[0230] The detection of embodiment 2 mutants

[0231] The adjacent sequence of T-DNA was queried by Tail-PCR.

[0232] Principle of TAIL-PCR technology: TAIL-PCR is also called thermal asymmetric staggered PCR. This technology designs three nested specific primers through the known sequence next to the target sequence, and uses them respectively with random degenerate primers (arbitrarydegenerateprime, AD) Combination, using genomic DNA as a template, performing three consecutive rounds of PCR cycles, using different annealing temperatures to selectively amplify target fragments, thereby amplifying adjacent unknown sequences from known sequences. This technique is widely used for isolation of flanking sequences from libraries of insertion mutants. The TAIL-PCR reaction system and procedures of this embodiment are as follows:

[0233] Tail-PCR1

[0234] ddH2O9.3μl10×Buffer2μlMg2+(2.5mM)2μl

[0235] dNTP (2.5mM) 1.5μl Arbitrary primer (20μM) 2μl

[0236] T-DNA1primer (2μM) ...

Embodiment 3

[0285] Example 3 Effect of OsREM6.6 gene on rice seed setting rate and yield

[0286] In the artificial climate chamber (setting parameters: 12 hours photoperiod, light intensity 200~250μmol·m -2 ·s -1 , temperature 28±1℃), and found that the OsREM6.6 gene directly regulates the seed setting rate and yield of rice.

[0287] At the seed maturity stage, the morphological indicators of wild type (WT) and mutant (M) were statistically analyzed, figure 2 It shows that the seed setting rate of the mutant is significantly reduced, (A) is the ear phenotype of the wild type and the mutant seed maturity stage; (B) is the seed setting rate (Seedsetting rate) of 10 independent lines of the wild type and the mutant , 1000-grain weight (1000-grainweight), spikelet number (Spikeletnumber), seed length (Grainlenght), seed width (Grainwidth), seed thickness (Grainthickness), tiller number (Tillernumber), plant height (Plantheight) and internode length (Internodelength ) to perform statisti...

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Abstract

The invention relates to a gene regulating and controlling rice seed setting rate and applications. Concretely, after selection of defective mutants of a lots of rice seeds, Remorin protein with specificity to plants and the coding gene OsREM6.6 of the Remorin protein participate in regulation and control of the seed setting rate, which is discovered firstly. Though regulation and control of the expression of the protein and the gene, rice with different seed setting rates can be obtained. The expression of the protein and the gene has tissue specificity: the protein and the gene are always expressed in companion cells of phloems, and the Remorin protein is specific to and steadily located on cell membranes.

Description

technical field [0001] The invention belongs to the field of biotechnology and breeding, in particular, the invention relates to a gene for regulating the seed setting rate of rice and its application. Background technique [0002] The food problem is a worldwide problem. With the rapid increase of population and the decrease of available arable land, it has become an important strategic measure for all countries to increase food production as soon as possible. Rice (Oryza sativa) is the main food variety and economic crop for Asian populations, and the improvement of rice yield is of great significance to solve the food problem. How to increase the yield of food to a greater extent has become an important topic of plant science research. The rapid acquisition of high-quality, high-yield and high-resistance new rice materials by using biotechnology for excellent genes has also attracted much attention. [0003] Rice yield is mainly determined by three factors: grain weight...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N15/84A01H5/00C12N15/113C12N15/63
Inventor 李来庚桂金山
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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