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A preparation method of fungal extract for inhibiting human immunodeficiency virus

A technology of extracts and fungi, which is applied in the field of preparation of endophytic fungal extracts, can solve problems such as environmental pollution and destruction, and depletion of plant resources

Inactive Publication Date: 2016-01-20
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, extensive exploitation of plants will lead to the depletion of plant resources, environmental pollution and destruction.

Method used

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  • A preparation method of fungal extract for inhibiting human immunodeficiency virus
  • A preparation method of fungal extract for inhibiting human immunodeficiency virus
  • A preparation method of fungal extract for inhibiting human immunodeficiency virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] 1. Fermentation of fungi

[0020] After the carbon group Q-s-4 (Hypoxylonsp.) was activated from the slant test tube strain preserved at low temperature, it was transferred to a plate containing PDA medium, cultured at a constant temperature of 25°C for 5 days, and holes were punched at the edge of the colony to form uniform diameters The bacterial flakes were inserted into the wheat bran liquid culture medium in small pieces.

[0021] The composition of the wheat bran liquid medium is: 20 g / L of wheat bran (cook for 30 minutes, extract the juice), 30 g / L of glucose, KH 2 PO 4 2 g / L, MgSO 4 1 g / l. 100mL medium in 250mL Erlenmeyer bottle, sterilized at 122°C for 22 minutes, inoculated with Charcoa Q-s-4 (Hypoxylonsp.) according to the above method, and cultivated in dark at 25°C for 14 days with shaking at 120 rpm.

[0022] 2. Obtaining of fungal fermentation products

[0023] After the fungus Q-s-4 was fermented and cultivated, it was filtered with a 100-mesh nylon...

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Abstract

The invention provides a preparation method of a fungal extract for inhibiting human immunodeficiency virus (HIV-1). The dependent bacterial strain is hypoxylon fungi Q-s-4(hypoxylon sp.). The fungi Q-s-4 is fermented and cultured in liquid to prepare extract of fermentation products. The method can be applied to the mass industrial culture of fungi Q-s-4 and industrial production of extract of fermentation products by taking fermentation products as the raw materials. The extract prepared by the method has a prominent activity on inhibiting HIV-1 integrase, an antibacterial activity, and an important value on research and development of novel drugs for curing AIDS and bacterial infections.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a preparation technology of an endophytic fungus extract capable of inhibiting the expression of human immunodeficiency virus (HIV-1) integrase. technical background [0002] Acquired immunodeficiency syndrome (Acquiredimmune deficiency syndrome, AIDs) is caused by infection with human immunodeficiency virus (Humanimmune deficiency virus, HIV). Since it was first reported by the Centers for Disease Control and Prevention in the United States in 1981, it has become one of the leading causes of death in the world. As of 2007, about 33.4 million people were infected with HIV worldwide, of which 22.4 million lived in sub-Saharan Africa, and about two-thirds of the population was infected with HIV. By the end of 2010, some 6.6 million people in low- and middle-income countries worldwide had received antiretroviral drug treatment, an almost 22-fold increase since 20...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A61K36/062A61P31/18C12R1/645
Inventor 郭顺星梁寒峭陈晓梅张大为王春兰邢咏梅李春艳李兵
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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