Cultivating method of hyriopsis cumingii with golden yellow shell

A technology of spinnaker mussels and cultivation methods, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of not paying attention to the breeding of mussels, environmental pollution, large economic losses, etc., and achieve the cultivation of pearls with good luster and shells. The effect of stable color heredity and high postoperative survival rate

Active Publication Date: 2014-01-01
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After more than 40 years of rapid development of my country's freshwater pearl breeding industry, a series of problems have gradually been exposed: due to the general lack of attention to the selection of parent mussels in the breeding process of S. Quality degradation and mixing, coupled with high-density cultivation, environmental pollution and other problems have caused frequent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment one: a kind of cultivation method of golden-yellow shell color Triangle sail clam, comprises the following steps:

[0025] (1) On March 10, 2010, 581 parent clams of the 4th to 6th instar with strong water spout, soft belly edge and complete shell were selected from the wild population of Poyang Lake. The male and female were identified by observing the gill filament spacer. 323 female mussels and 258 male mussels were selected, and the shell surfaces of the selected male and female broodstock were marked;

[0026] (2) Take 50 mg of the mantle edge tissue of male and female mussels, and use the column type animal tissue total RNA extraction and purification kit (Shanghai Sangon Bioengineering Co., Ltd.) to extract total RNA; use the AMV first-strand cDNA synthesis kit to synthesize single Strand cDNA; SRAP amplification was carried out with a specific primer combination, and the amplified products were subjected to agarose electrophoresis and observed under u...

Embodiment 2

[0032] Embodiment two: a kind of cultivation method of golden-yellow shell color triangular sail clam, comprises the following steps:

[0033] (1) On March 20, 2011, 483 parent clams of the 4th to 6th instar with strong water spout, soft belly edge and complete shell were selected from the wild population of Poyang Lake. The male and female were identified by observing the gill filament spacer. 272 female mussels and 211 male mussels were obtained, and the shell surfaces of the selected male and female broodstock were marked;

[0034] (2) Take 50 mg of the mantle edge tissue of male and female mussels, and use the column type animal tissue total RNA extraction and purification kit (Shanghai Sangon Bioengineering Co., Ltd.) to extract total RNA; use the AMV first-strand cDNA synthesis kit to synthesize single Strand cDNA; SRAP amplification was carried out with a specific primer combination, and the amplified products were subjected to agarose electrophoresis and observed under...

Embodiment 3

[0040] Embodiment three: a kind of cultivation method of golden-yellow shell color Triangle sail clam, comprises the following steps:

[0041] (1) On March 20, 2012, 1,710 parent mussels of the 4th to 6th instar with strong water spout, soft belly edge and complete shell were selected from the wild population of Poyang Lake, and male and female were identified by observing the gill filament spacer. 1124 female mussels and 586 male mussels were produced, and the shell surfaces of the selected male and female broodstock were marked;

[0042] (2) Take 50 mg of the mantle edge tissue of male and female mussels, and use the column type animal tissue total RNA extraction and purification kit (Shanghai Sangon Bioengineering Co., Ltd.) to extract total RNA; use the AMV first-strand cDNA synthesis kit to synthesize single Strand cDNA; SRAP amplification was carried out with a specific primer combination, and the amplified products were subjected to agarose electrophoresis and observed ...

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Abstract

The invention relates to a cultivating method of hyriopsis cumingii with a golden yellow shell. The method is characterized by comprising the following steps of (1), selecting 4-6 parent hyriopsis cumingii; (2) taking the margin tissues of the pallium of the parent hyriopsis cumingii, extracting the total RNA (ribonucleic acid) and synthetic single stranded c RNA, performing SRAP (sequence-related amplified polymorphism) amplification by adopting primer combination, and selecting male and female individuals with specific strip sequences with the golden yellow shells after the amplification products are subjected to electrophoresis; (3) using the selected male and female parent hyriopsis cumingii to build a family and cultivating in a water flowing pool; (4) periodically examining the pregnant condition of the female hyriopsis cumingii, and if mature glochidiums are found, selecting the mature female hyriopsis cumingii to reproduce to obtain infant hyriopsis cumingii; cultivating the infant hyriopsis cumingii in the water flowing pool until the shell reaches 1-2cm long, removing into to a net cage, and suspending the net cage into a pond to perform second stage cultivating on the infant hyriopsis cumingii until the shell length reaches 5-8cm; (5) removing the family parent hyriopsis cumingii with the non-golden yellow shells, and using the rest parent hyriopsis cumingii to build a core cultivating population with the golden yellow shells. According to the cultivation method of the hyriopsis cumingii with the golden yellow shell, the ratio of the hyriopsis cumingii with the golden yellow shell can reach more than 99.0%, the shell color is stable in heredity and the postoperative survival rate is high.

Description

technical field [0001] The invention relates to a method for cultivating golden-yellow-shelled triangular sail clams, belonging to the technical field of aquatic breeding. Background technique [0002] Triangle clams ( Hyriopsis cumingii (Lea) belongs to the genus Sail (Unioninae) of the clam family (Unionidae) Hyriopsis ), is endemic to my country. It is mainly distributed in large and medium-sized water bodies with micro-currents such as lakes and tributaries in the Yangtze River Basin and Huaihe River Basin. The five freshwater lakes of Poyang Lake, Dongting Lake, Taihu Lake, Chaohu Lake and Hongze Lake are particularly rich in resources. In the 1970s, the artificial breeding of S. sinensis in my country was successful. In the following years, the triangular sail clam replaced the pleated crested clam ( Cristaria plicata ), Anodonta dorsalis ( Anodonta woodiana woodiana ), etc., are used for the cultivation of freshwater pearls and have become the main freshwater...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 闻海波顾若波曹哲明华丹徐跑金武
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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