Application of protein CD38 to preparation of rheumatoid arthritis diagnosing marker

A diagnostic marker, rheumatoid technology, applied in the fields of disease diagnosis, biological testing, biological material analysis, etc., can solve the problems of high false positive rate, long time, and many experimental steps.

Inactive Publication Date: 2014-01-15
常晓天
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Problems solved by technology

Since the means of detecting RF and anti-CCP are based on the ELISA technology of antigen-antibody specific binding, there are many experimental steps, time-consuming, and high false positive rate
In addition, since my co

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  • Application of protein CD38 to preparation of rheumatoid arthritis diagnosing marker

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Embodiment 1

[0012] Example 1 Detecting the expression of CD38 in RA peripheral blood by flow cytometry

[0013] The applicant of the present invention collected blood samples by venipuncture from 103 RA patients aged 20-74, 75 of whom were female. Peripheral blood was collected into EDTA-K2 anticoagulated vacuum blood collection tubes. All patients were selected in accordance with the American College of Rheumatology (ACA) diagnostic criteria for RA: chronic inflammation in the joints of all patients for more than three years, erythrocyte sedimentation rate (ESR), C-reactive protein ( C-reactive protein, CRP) and rheumatoid factor (rheumatoid factor, RF) detection values ​​were significantly higher than the normal range, respectively 19 ~ 22mm / H, 8 ~ 196U / ml and 10.5 ~ 2,090U / ml. The study took 120 healthy people aged 24-58, including 80 women, as controls.

[0014] The applicant of the present invention took 200 μl of peripheral blood and 20 μl of the corresponding flow-type antibody a...

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Abstract

The invention discloses a novel rheumatoid arthritis diagnosing marker-protein CD38. Through flow cytometrical experiments, the applicant of the invention proves that compared with normal subjects, CD38+ cells are highly expressed in peripheral blood lymphocytes of a RA (rheumatoid arthritis) patient, and the expression levels of the CD38+ cells are significantly related to the RF (rheumatoid factor) level. In addition, flow cytometrical results show that the ratio of a CD38+ cell subset to a CD56+ cell subset is also significantly increased and the CD56 is a cell surface marker of a natural killer cell (NK cell), which means that the CD38 plays an important role in autoimmune disorder of the RA and can be used as the rheumatoid arthritis diagnosing marker. A kit for detecting the rheumatoid arthritis consists of CD3 marked by FITC, a CD4 monoclonal antibody, a CD56 monoclonal antibody marked by PE, and a CD38 monoclonal antibody marked by Percp-Cy5.5.

Description

technical field [0001] The present invention relates to the use of protein CD38 as / preparing a diagnostic marker for rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is an autoimmune disease with an incidence rate of 3.5 per thousand. At present, clinical diagnosis of RA is provided by detecting rheumatic factor (rheumatic factor, RF) and anti-citrullinated autoimmune antibody (anti-CCP) in peripheral blood of patients. Since the means of detecting RF and anti-CCP are based on the ELISA technology of antigen-antibody specific binding, there are many experimental steps, time-consuming, and high false positive rate. In addition, since my country does not have the intellectual property rights of the above two detection markers, the market price of the kit is more than 4,000 yuan, and the testing fee per person in the hospital is more than 200 to 260 yuan. [0003] The main lesion site of RA is the joint synovium. The applicants of the present inv...

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/564G01N2800/102
Inventor 常晓天
Owner 常晓天
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