Method for purification and rejuvenation of potato virus-free tissue culture seedlings

A technology for purifying and rejuvenating potatoes, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of high production cost of virus-free potato original seed tubers, high investment cost of culture medium, and impact on economic benefits, etc., to achieve Achieve economic recycling, enhance drought resistance, and improve economic benefits

Active Publication Date: 2014-01-22
GANSU KAIKAI AGRI TECH DEV
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  • Description
  • Claims
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Problems solved by technology

However, the virus-free original seed potato must be obtained by tissue culture. At present, the investment cost of the medium used in potato tissue culture technology is high, which seriously affe

Method used

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  • Method for purification and rejuvenation of potato virus-free tissue culture seedlings
  • Method for purification and rejuvenation of potato virus-free tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. The composition of the medium for potato virus-free tissue culture includes:

[0025] Compound carrageenan: 100g / L;

[0026] Macroelements: NH 4 NO 3 1.0g / L, KNO 3 1.3g / L, CaCl 2 4H 2 O0.3g / L, MgSO 4 ·7H 2 O0.2g / L, KH 2 PO 4 0.1g / L;

[0027] Trace elements: KI0.2mg / L, H 3 BO 3 1.0mg / L, MnSO 4 4H 2 O5.5mg / L, ZnSO 4 4H 2 O2.0mg / L, Na 2 MoO 4 2H 2 O0.04mg / L, CuSO 4 ·5H 2 O0.008mg / L, CoCl 2 ·6H 2 O0.008mg / L, FeSO 4 ·7H 2 O9.5mg / L, CrCl 3 ·6H 2 O0.009mg / L;

[0028] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0029] Organic ingredients: inositol 25mg / L, sodium benzoate 24mg / L;

[0030] Others: 30g / L sucrose, appropriate amount of deionized water.

[0031] Production of compound carrageenan: (1) Recovered chicken manure is deodorized by high-temperature drying; (2) Add appropriate amount of deionized water to carrageenan, and heat up to 70-80°C until it is completely dissolved; (3) Dry and deodorize chicken Add deionized water to ...

Embodiment 2

[0045] The composition of the medium for potato virus-free tissue culture includes:

[0046] Compound carrageenan: 110g / L;

[0047] Macroelements: NH 4 NO 3 1.1g / L, KNO 3 1.4g / L, CaCl 2 4H 2 O0.33g / L, MgSO 4 ·7H 2 O0.22g / L, KH 2 PO 4 0.11g / L;

[0048] Trace elements: KI0.22mg / L, H 3 BO 3 1.3mg / L, MnSO 4 4H 2 O5.8mg / L, ZnSO 4 4H 2 O2.2mg / L, Na 2 MoO 4 2H 2 O0.04mg / L, CuSO 4 ·5H 2 O0.009mg / L, CoCl 2 ·6H 2 O0.009mg / L, FeSO 4 ·7H 2 O9.8mg / L, CrCl 3 ·6H 2 O0.009mg / L;

[0049] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0050] Organic ingredients: inositol 25mg / L, sodium benzoate 24.5mg / L;

[0051] Others: 30g / L sucrose, appropriate amount of deionized water.

[0052] The preparation method of medium for potato virus-free tissue culture is the same as example 1.

[0053] Potato virus-free tissue culture seedling cultivation method is the same as example 1.

Embodiment 3

[0055] The composition of the medium for potato virus-free tissue culture includes:

[0056] Compound carrageenan: 120g / L;

[0057] Macroelements: NH 4 NO 3 1.2g / L, KNO 3 1.5g / L, CaCl 2 4H 2 O0.35g / L, MgSO 4 ·7H 2 O0.25g / L, KH 2 PO 4 0.12g / L;

[0058] Trace elements: KI0.25mg / L, H 3 BO 3 1.5mg / L, MnSO 4 4H 2 O6.0mg / L, ZnSO 4 4H 2 O2.5mg / L, Na 2 MoO 4 2H 2 O0.05mg / L, CuSO 4 ·5H 2 O0.01mg / L, CoCl 2 ·6H 2 O0.01mg / L, FeSO 4 ·7H 2 O10.0mg / L, CrCl 3 ·6H 2 O0.01mg / L;

[0059] Growth regulator: sodium α-naphthalene acetate 7.5mg / L;

[0060] Organic ingredients: inositol 25mg / L, sodium benzoate 25mg / L;

[0061] Others: 30g / L sucrose, appropriate amount of deionized water.

[0062] The preparation method of medium for potato virus-free tissue culture is the same as example 1.

[0063] Potato virus-free tissue culture seedling cultivation method is the same as example 1.

[0064] Adopt the assay method of example 1, when counting different medium element ad...

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Abstract

The invention discloses a method for purification and rejuvenation of potato virus-free tissue culture seedlings. The method is characterized in that a culture medium suitable for growth of potato tissue culture seedlings is prepared through an MS culture medium, composite carrageenan and alpha-pimacol, the pre-cultured virus-free tissue culture seedlings are placed in the solid culture medium and cultured for 12 days, roots and stems of the virus-free potato tissue culture seedlings are strong, and the survival rate of seedling acclimatization and transplantation is high. Compared with an existing potato tissue culture seedling medium, the culture medium has the advantages of being low in cost and good in economic benefit.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for purifying and rejuvenating potato virus-free tissue-cultured seedlings. Background technique [0002] Potato original seed detoxification can effectively increase yield and improve quality, so the detoxification technology of potato original seed has been more and more widely used. However, the virus-free original seed potato must be obtained by tissue culture. At present, the investment cost of the medium used in potato tissue culture technology is high, which seriously affects the economic benefit. All kinds of elements, especially the expensive trace elements, caused the production cost of the original seed potato of virus-free potato to be too high and could not be widely used. At the same time, the potato virus-free tissue-cultured seedlings are directly hardened and transplanted after differentiation and cultivation. The robustness and qu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李恺屠建章张才强吴成秀火有仁
Owner GANSU KAIKAI AGRI TECH DEV
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