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A method for ultra-low temperature freezing and storage of sperm of golden perch

A technology of ultra-low temperature freezing and perch, which is applied in the field of animal reproduction, can solve the problems of difficult implementation of genetic selection and hybrid combination, impossibility of artificial insemination, asynchronous gonad development, etc., to prolong the survival time in vitro, solve the problem of less semen, and maintain fertilization Vitality effect

Active Publication Date: 2015-08-19
SUZHOU FISHSEEDS BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large individual differences between male and female fish and physiological defects such as asynchronous gonad development, it is often difficult to obtain mature sperm and mature eggs at the same time, and artificial insemination cannot be carried out, making genetic selection and hybridization difficult to implement.

Method used

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  • A method for ultra-low temperature freezing and storage of sperm of golden perch
  • A method for ultra-low temperature freezing and storage of sperm of golden perch
  • A method for ultra-low temperature freezing and storage of sperm of golden perch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The sperm ultralow temperature freezing storage method of golden perch comprises the following steps:

[0018] 1. Semen collection

[0019] Grab the male fish and put it in a dry towel, after absorbing the surface water, squeeze the sides of its abdomen with your fingers, and after the semen flows out, use a 75% alcohol-sterilized plastic dropper to absorb the semen;

[0020] Sperm motility detection: Take 2 μL of semen, add 20 μL of deionized water to mix, put it on a glass slide, cover the glass slide, and use an optical microscope to detect. Only sperm that can move forward are considered viable (sperm that are still moving in the same place are not). considered as inactive), the motility was calculated as a percentage, and the measured sperm motility was 85.33%.

[0021] 2. Semen frozen storage:

[0022] (1) Frozen storage solution

[0023] formula:

[0024]

[0025] Its preparation method comprises the following steps:

[0026] a. Put 600mL deionized water ...

Embodiment 2

[0043] The sperm ultralow temperature freezing storage method of golden perch comprises the following steps:

[0044] 1. Semen collection

[0045] Grab the male fish and put it in a dry towel, after absorbing the surface water, squeeze the sides of its abdomen with your fingers, and after the semen flows out, use a 75% alcohol-sterilized plastic dropper to absorb the semen;

[0046] Sperm motility detection: Take 2 μL of semen, add 20 μL of deionized water to mix, put it on a glass slide, cover the glass slide, and use an optical microscope to detect. Only sperm that can move forward are considered viable (sperm that are still moving in the same place are not). considered as inactive), the motility was calculated as a percentage, and the measured sperm motility was 70.21%.

[0047] 2. Semen frozen storage:

[0048] (1) Frozen storage solution

[0049] formula:

[0050]

[0051]

[0052] Its preparation method comprises the following steps:

[0053] a. Put 600mL deio...

Embodiment 3

[0070] The sperm ultralow temperature freezing storage method of golden perch comprises the following steps:

[0071] 1. Semen collection

[0072] Grab the male fish and put it in a dry towel, after absorbing the surface water, squeeze the sides of its abdomen with your fingers, and after the semen flows out, use a 75% alcohol-sterilized plastic dropper to absorb the semen;

[0073] Sperm motility detection: Take 2 μL of semen, add 20 μL of deionized water to mix, put it on a glass slide, cover the glass slide, and use an optical microscope to detect. Only sperm that can move forward are considered viable (sperm that are still moving in the same place are not). considered as inactive), the motility was calculated as a percentage, and the measured sperm motility was 75.79%.

[0074] 2. Semen frozen storage:

[0075] (1) Frozen storage solution

[0076] formula:

[0077]

[0078] Its preparation method comprises the following steps:

[0079] a. Put 600mL deionized water ...

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Abstract

The invention provides a super-freezing storage method of yellow perch sperm. The method comprises the following steps: storing seminal fluid at low temperature: uniformly mixing the collected seminal fluid of yellow perch with storage liquid to obtain sperm storage liquid, and refrigerating at 2-6 DEG.C; and super-freezing for storing the seminal fluid, namely uniformly mixing the sperm storage liquid with a cryoprotectant, cooling in stages, and then storing in liquid nitrogen. The super-freezing storage method of the yellow perch sperm is simple in process, low in cost, and capable of effectively prolonging the in vitro survival time of the yellow perch sperm, protecting the integrity of the sperm, improving the survival index of the yellow perch sperm obviously and keeping the fertilization vitality of the sperm.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and in particular relates to a method for ultra-low temperature freezing and storage of sperm of golden perch. Background technique [0002] Golden perch (Perca flavescens), widely distributed, is a freshwater fish with high nutritional value distributed in the Great Lakes of North America. It used to be the largest fishery industry in North American history. In recent years, due to overfishing, water pollution and other reasons, the catch of golden perch has dropped sharply, causing the wild catch to be far from meeting the market demand. In order to make up for this lack, the artificial breeding industry of golden perch has developed rapidly. However, compared with other cultured fish, golden perch has a slower growth rate, significant growth differences between male and female fish, asynchronous development, and premature sexual maturity (the first age Mature), most males are diff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 叶旭红邱崇文林先贵刘青华
Owner SUZHOU FISHSEEDS BIOLOGICAL TECH CO LTD
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