Particle fluorescence detection wavelength instant configuration beam splitting system

Abstract

The invention discloses a particle fluorescence detection wavelength instant configuration beam splitting system which comprises a laser light source and an optical shaping unit, wherein the optical shaping unit is arranged in front of the laser light source; a fluid forming unit is arranged in front of the optical shaping unit; an acousto-optic tunable filter is arranged in front of the fluid forming unit and is connected with a radio frequency driver; the fluid forming unit comprises sheath fluid, a sample, particles and a particle detection area. According to the technical scheme, optional selection of central wavelength, bandwidth control of a fluorescence channel and modulation of fluorescence transmittance in an operating range of the acousto-optic tunable filter are realized in a particle fluorescence detection process according to the dye types and a to-be-detected target fluorescence wavelength channel needing to be dynamically configured; the defect that the fluorescence detection beam-splitting channel in conventional particle fluorescence detection beam-splitting structures such as a flow cytometry is relatively fixed and is difficult to adjust is overcome; the system can be unique or is combined with an optical filter beam-splitting method to serve as a beam-splitting scheme for particle fluorescence detection.

Description

technical field [0001] The invention relates to the field of sample fluorescence analysis methods and devices, in particular to a spectroscopic system for real-time configuration of particle fluorescence detection wavelengths. Background technique [0002] Fluorescence analysis methods are commonly used in cell biology research. In this method, if the wavelength resolution ability is strong, it is helpful for the multi-parameter analysis and identification of various dyes. Because different fluorescent dyes have different application fields, multiple dyes are usually used in combination to better analyze surface antibodies, nucleic acid sequences, and protein types. Therefore, the use of detection channels that can dynamically configure fluorescence wavelength parameters helps the system to adapt to different dye types. , to obtain better analysis results. [0003] The above fluorescence analysis method is typically applied to a flow cytometry analysis method. Flow cytome...

AI Technical Summary

Problems solved by technology

However, due to the spectral scheme of the traditional fluorescence parameter channel, the filter system with fixed central wavelength and bandwidth is used to achieve different fluorescence separations.

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