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Herba epimedii polysaccharide and components thereof as well as use of herba epimedii polysaccharide and components thereof as vaccine adjuvants

A technology of Epimedium polysaccharide and Epimedium, which is applied to medical preparations, drug combinations, antibody medical components and other directions containing active ingredients, can solve the problem of insignificant synergy, achieve good adjuvant effect, and enhance immune response. Effect

Active Publication Date: 2014-03-05
BEIJING ZHONGAN ADJUVANT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results showed that the mixture could increase the antibody titer and IFN-γ mRNA expression level, but the synergistic effect with IL-2 was not obvious

Method used

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  • Herba epimedii polysaccharide and components thereof as well as use of herba epimedii polysaccharide and components thereof as vaccine adjuvants
  • Herba epimedii polysaccharide and components thereof as well as use of herba epimedii polysaccharide and components thereof as vaccine adjuvants
  • Herba epimedii polysaccharide and components thereof as well as use of herba epimedii polysaccharide and components thereof as vaccine adjuvants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1: Preparation of Epimedium total polysaccharide

[0080] Epimedium leaves 1kg, soaked in petroleum ether at room temperature for 24 hours, filtered, and the filtrate was decompressed at 30-35°C to recover the extract. After the epimedium leaves extracted by petroleum ether evaporated naturally, add 15L of distilled water, soak for 48 hours at room temperature (20-30°C), stir occasionally during this period; then filter, centrifuge the filtrate for 10min (speed 3000r / min), The extracted Epimedium leaves were extracted twice under the same conditions. Combine the water extracts extracted twice, concentrate under reduced pressure at 50-55°C to 1000ml, and then add 3 times the volume (3000ml) of 95% ethanol for alcohol precipitation for 48 hours. Centrifuge the alcohol precipitation solution for 10 minutes (3000r / min), add 1000ml of water to the precipitated part, stir to dissolve, centrifuge, and perform the same operation twice for the precipitated part. C...

Embodiment 2

[0081] Embodiment 2: Preparation of Epimedium total polysaccharide

[0082]Epimedium leaves 1kg, add 15L distilled water at room temperature, soak at room temperature (20-30°C) for 48 hours, stir occasionally; then filter, centrifuge the filtrate for 10min (speed 3000r / min), the extracted Epimedium leaves in A second extraction was carried out under the same conditions. Combine the water extracts extracted twice, concentrate under reduced pressure at 50-55°C to 1000ml, and then add 3 times the volume (3000ml) of 95% ethanol to carry out alcohol precipitation for 48-72 hours. Centrifuge the alcohol precipitation solution for 10 minutes (3000r / min), add 1000ml of water to the precipitated part, stir to dissolve, centrifuge, and perform the same operation twice for the precipitated part. Combine the dissolved supernatants, put them into a dialysis bag (molecular weight cut-off > 1000), dialyze with tap water for 48 hours, then change to distilled water and then dialyze for 24 ...

Embodiment 3

[0083] Embodiment 3: Preparation of Epimedium total polysaccharide

[0084] Epimedium leaves 1kg, add 15L of distilled water at 55-60°C, soak for 48 hours, stirring from time to time; then filter, centrifuge the filtrate for 10min (speed 3000r / min), extract the epimedium leaves under the same conditions Perform a secondary extraction. Combine the water extracts extracted twice, concentrate under reduced pressure at 50-55°C to 1000ml, and then add 3 times the volume (3000ml) of 95% ethanol to carry out alcohol precipitation for 48-72 hours. Centrifuge the alcohol precipitation solution for 10 minutes (3000r / min), add 1000ml of water to the precipitated part, stir to dissolve, centrifuge, and perform the same operation twice for the precipitated part. Combine the dissolved supernatants, put them into a dialysis bag (molecular weight cut-off > 1000), dialyze with tap water for 48 hours, then change to distilled water and then dialyze for 24 hours. The dialysate was concentrat...

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Abstract

The invention relates to a polysaccharide extract of herba epimedii, and specifically relates to herba epimedii total polysaccharides and acid saccharide components and non-saccharide components in the herba epimedii total polysaccharides extracted from a traditional Chinese medicinal material herba epimedii as well as use of herba epimedii polysaccharide and the components thereof in preparation of vaccine adjuvants, vaccine preparations, vaccine compositions or antibodies. The invention further relates to pharmaceutical compositions, vaccine adjuvants, vaccine preparations or vaccine compositions comprising herba epimedii total polysaccharides and components of herba epimedii total polysaccharides.

Description

[0001] This application is based on the divisional application of the Chinese patent application with the application number 201110220409.0, the application date is August 3, 2011, and the invention title is "Epimedium polysaccharide and its components and their use for vaccine adjuvants". technical field [0002] The present invention relates to the polysaccharide extract of Epimedium, specifically, relates to the total polysaccharide of Epimedium extracted from the leaves of Chinese herbal medicine Epimedium and the acidic sugar component and non-sugar component thereof, and the present invention also relates to said Epimedium The application of the sheep gown total polysaccharide and its acid sugar component and non-sugar component in vaccine adjuvant, vaccine composition and antibody preparation. Background technique [0003] Epimedium (Epimedium) is a plant of Berberidaceae (Berberidaceae), which is often used for tonifying kidney and strengthening yang, dispelling wind ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K39/39A61K31/715A61P37/04C07K16/16A61K39/395
CPCA61K2039/55583C08B37/0003A61K39/39A61K31/715C08B37/00
Inventor 单俊杰王玉霞朱婷贾培媛武军华赵修南刁玉林王晨宇段丹丹王佩瑞
Owner BEIJING ZHONGAN ADJUVANT BIOTECH
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