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Serratia marcescens strain and synchronous extraction and fermentation method thereof

A technology of Serratia marcescens and strains, which is applied in the field of fermentation industry, can solve the problems of no simultaneous extraction and fermentation, and achieve the effect of large-scale industrial application, increased production, and simple and fast purification and recovery process

Inactive Publication Date: 2014-03-12
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The publication numbers are CN101392227B (a Serratia marcescens and the prodigiosin produced), CN102002469A (the name of the invention: a strain for producing prodigiosin and its method), CN102277323A (the name of the invention: a high-yield prodigiosin Serratia marcescens (Serratia marcescens) Sm-128 strain and its application) and other patents have announced a series of prodigiosin-producing Serratia marcescens strains and methods for producing prodigiosin. The production methods are Conventional liquid fermentation method, no reports on simultaneous extraction and fermentation

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  • Serratia marcescens strain and synchronous extraction and fermentation method thereof
  • Serratia marcescens strain and synchronous extraction and fermentation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] : Screen and isolate Serratia marcescens Xd-1 from moldy tofu.

[0019] (1) Culture medium: skim milk medium: skim milk powder 100g / L, agar powder 20g / L, natural pH.

[0020] (2) Screening and isolation of bacterial strains: Take 2 g of moldy tofu surface samples, add to 100 mL of sterile water, shake at 30°C and 150 r / min for 30 min. Using concentration gradient dilution method (10 -1 -10 -4 ), spread 100uL of the diluted solution on a skim milk medium plate, and incubate at 30°C for 24h. A single colony that was white in the early stage and red in the later stage was picked, and separated by 5 consecutive streaks to obtain the Xd-1 strain with a single character. Morphology, physiology and biochemistry, and 16S ribosomal RNA gene (16S rDNA) sequence identification were carried out on Xd-1.

[0021] (3) Colony and cell morphology

[0022] After the bacteria were cultured on LB medium at 30°C for 12 hours, the surface of the colony was smooth, moist, and round. Wit...

Embodiment 2

[0029] : Prodigiosin fermentation blank control experiment

[0030] (1) The culture medium formula of this embodiment is:

[0031] LB slant medium (g / L): tryptone 10, yeast powder 5, NaCl 10, agar powder 15.

[0032] Liquid seed medium (g / L): tryptone 10, yeast powder 5, NaCl10, that is, LB liquid medium.

[0033] Liquid fermentation medium (g / L): glycerol 15, peptone 6.

[0034] (2) Serratia marcescens Xd-1 was inoculated on LB slant medium at a culture temperature of 30°C for 24 hours, and then inoculated into liquid seed medium. The cultivation conditions of the liquid seeds are as follows: cultivation temperature 30°C, shaker rotation speed 180r / min, cultivation time 12h, to obtain the first-grade seed liquid.

[0035] According to the 10% inoculation amount, put the primary seed liquid into the 3L seed tank, and add 2L seed medium to the 3L seed tank. The culture temperature is 30°C, the rotation speed of the shaker is 180r / min, the ventilation rate is 1.5v / (v.m), and...

Embodiment 3

[0037] : Synchronous extraction and fermentation of prodigiosin

[0038] (1) The culture medium formula of this embodiment is:

[0039] LB slant medium (g / L): tryptone 10, yeast powder 5, NaCl 10, agar powder 15.

[0040] Liquid seed medium (g / L): tryptone 10, yeast powder 5, NaCl10, that is, LB liquid medium.

[0041] Liquid fermentation medium (g / L): glycerol 8, peptone 3.

[0042] (2) Serratia marcescens Xd-1 was inoculated on LB slant medium at a culture temperature of 30°C for 24 hours, and then inoculated into liquid seed medium. The cultivation conditions of the liquid seeds are as follows: the cultivation temperature is 30° C., the rotation speed of the shaker is 180 r / min, and the cultivation time is 12 hours.

[0043] According to the 10% inoculation amount, put the primary seed liquid into the 3L seed tank, and add 2L seed medium to the 3L seed tank. The culture temperature is 30°C, the rotation speed of the shaker is 180r / min, the ventilation rate is 1.5v / (v.m), ...

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Abstract

The invention provides a Serratia marcescens Xd-1 strain with an accession number of CGMCC No. 7734 and a synchronous extraction and fermentation production method for prodigiosin by using the strain. The method is characterized by comprising the following steps: preparing primary seed liquid of the strain; preparing secondary seed liquid of the strain; mixing a liquid fermentation medium with a volume of V and the secondary seed liquid with a volume of 1 to 8% of V in a container and carrying out fermentation for 6 to 18 h under the fermentation conditions of a rotating speed of 130 to 200 r / min, oxygen flux of 0.5 to 1.5 v / (v.m) and fermentation temperature of 25 to 35 DEG C; and adding an extractant with a volume of 8 to 20% of V into the container and continuing fermentation under the fermentation conditions, wherein total fermentation time is 32 to 64 h.

Description

technical field [0001] The invention relates to a strain of Serratia marcescens with high prodigiosin production and a synchronous extraction and fermentation method thereof, in particular to a synchronous extraction and fermentation production method of prodigiosin, which belongs to the field of fermentation industry. Background technique [0002] Prodigiosin is a family of natural red pigments with multiple pyrrole ring structures. It was discovered by Amak et al. in 1929, and was isolated and purified for the first time by Harashimak et al. Early research focused on antimicrobial, antimalarial and other biological activities. In recent years, prodigiosin has been widely concerned by many researchers at home and abroad because of its excellent biological activities such as anti-tumor and immunosuppression, and it is a very potential biological agent. [0003] Prodigiosin has cytotoxic activity on various malignant tumors, such as targeting and inducing apoptosis on human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/16C12R1/43
Inventor 艾连中夏永军徐斐侯建平陈卫
Owner UNIV OF SHANGHAI FOR SCI & TECH
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