A kind of extraction method of apigenin, pharmaceutical composition and application thereof for treating diabetes

Abstract

The invention relates to the pharmaceutical technical field. The extraction method of apigenin comprises the steps of 1) enzymatically hydrolyzing celery which is taken as a substrate for 10 hours at 30-35 DEG C through a mixing solution of apigenin which is 33-37% and glucosidase which is 43-47% in mass percentage concentration; and 2) ultrasonically extracting enzymatic hydrolysate obtained from the step 1) through an ethanol solution of 70%, condensing an extracting solution, extracting respectively through petroleum ether and ethyl acetate, evaporating the extracting solution, dissolving through ethanol of 70%, and separating and extracting through a high performance liquid chromatograph. The pharmaceutical composition for treating diabetes consists of apigenin and glucobay at mass ratio of 1: (2-5). The extraction method, which extracts apigenin through a multi-enzymatic process, greatly improves extraction yield. The pharmaceutical composition disclosed by the invention not only can effectively control blood sugar but also can avoid circumstances of low blood sugar and rapid weight loss of a patient due to simple use of a diabetes medicine.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to a method for extracting apigenin, a pharmaceutical composition added with apigenin for treating diabetes and an application thereof. Background technique [0002] With the improvement of people's living standards, diabetes has become a global public health problem, and it has also caused direct problems to the health of our people. Diabetes itself can not only cause death, but also its complications such as foot rot, diabetic nephropathy, high blood pressure, and cardiovascular disease have expanded the harm of diabetes to all areas of the human body. Currently, there are 246 million people with diabetes worldwide, of which type 2 diabetes accounts for 90%. China currently estimates that there are 92.4 million people with diabetes over the age of 20, ranking among the highest in the world. Therefore, the treatment and prevention of diabetes has become an urgent problem. In ad...

AI Technical Summary

Problems solved by technology

Although insulin treatment of diabetes can effectively control the blood sugar of diabetic patients, there are several recognized defects in this treatment: (1) too much fluctuation of blood sugar; (2) weight gain; (3) poor control of blood sugar after meals; (4) ) with symptoms of severe hypoglycemia

However, there are also certain problems in this combination drug: (1) Due to the slow absorption and decomposition of carbohydrates in the small intestine and prolonged residence time, patients often have side effects such as abdominal pain and diarrhea, and because the patient loses too much weight, although the patient's The blood sugar concentration has been controlled to a certain extent, but the immunity of the patient's intestinal tract has declined; (2) Although Baitangping can reduce the risk of hypoglycemia caused by insulin alone to a certain extent, the combination of drugs cannot significantly reduce this risk. risk

Although the combination of insulin and Baitangping can effectively control the blood sugar level of diabetic patients, hypoglycemia, diarrhea and rapid weight loss in diabetic patients also bring hidden concerns to the treatment

In addition, regarding the extraction of apigenin, in the past, an enzyme that cracks the plant cell wall was basically used for extraction, but the enzymes are specific, so only one enzyme can only make the cell wall of plant tissue incompletely cracked, such as pectin Enzyme, it can only hydrolyze the pectin in the plant cell wall into galacturonic acid, and there are a lot of other substances such as cellulose in the plant cell wall, so in order to extract apigenin efficiently, it is necessary to extract the plant celery complete cell wall breakdown

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