Extraction method of apigenin, pharmaceutical composition for treating diabetes and application thereof

An extraction method, apigenin technology, is applied in the direction of drug combination, medical preparations containing active ingredients, metabolic diseases, etc., and can solve problems such as treatment hidden worries, prolonged residence time, and decreased immunity of patients

Active Publication Date: 2014-03-19
南通大学技术转移中心有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although insulin treatment of diabetes can effectively control the blood sugar of diabetic patients, there are several recognized defects in this treatment: (1) too much fluctuation of blood sugar; (2) weight gain; (3) poor control of blood sugar after meals; (4) ) with symptoms of severe hypoglycemia
However, there are also certain problems in this combination drug: (1) Due to the slow absorption and decomposition of carbohydrates in the small intestine and prolonged residence time, patients often have side effects such as abdominal pain and diarrhea, and because the patient loses too much weight, although the patient's The blood sugar concentration has been controlled to a certain extent, but the immunity of the patient's intestinal tract has declined; (2) Although Baitangping can reduce the risk of hypoglycemia caused by insulin alone to a certain e

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  • Extraction method of apigenin, pharmaceutical composition for treating diabetes and application thereof
  • Extraction method of apigenin, pharmaceutical composition for treating diabetes and application thereof
  • Extraction method of apigenin, pharmaceutical composition for treating diabetes and application thereof

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Example Embodiment

[0028] The extraction of embodiment 1 apigenin and the extraction method of apigenin of the present invention compare with existing extraction method

[0029] (1) Extract apigenin:

[0030] 1) Using celery leaves as a substrate, use a mixed solution of 37% pectinase and 47% glucosidase in mass percent concentration to enzymolyze at 35°C for 10 hours. The amount of enzyme mixed solution added is 5g of celery leaves 15mL;

[0031]2) The enzymolysis product obtained in step 1) was ultrasonically extracted with 70% ethanol solution for 3 times, each time for 30 minutes, the power of the ultrasonic wave was 80W, and the temperature was 57°C. Equal volumes of petroleum ether and ethyl acetate were extracted separately, the extract was evaporated to dryness, dissolved in 70% ethanol, and finally separated and extracted by high performance liquid chromatography to obtain apigenin.

[0032] (2) Extract apigenin:

[0033] 1) Using celery leaves as a substrate, use a mixed solution of...

Example Embodiment

[0046] Embodiment 2 prepares pharmaceutical composition

[0047] Pharmaceutical composition one: Formula: 10g of apigenin, 20g of Baitangping. (Apigenin: Baitangping=1:2).

[0048] The above-mentioned pharmaceutical composition is prepared, comprising the following steps:

[0049] 1) Extraction of apigenin: using celery leaves as a substrate, hydrolyze with 35% pectinase and 45% glucosidase at 34°C for 10 hours, then ultrasonically extract 3 times with 70% ethanol solution, 30% each time minutes, the ultrasonic power is 80W, and the temperature is 52°C. Concentrate the extract to 100mL with a rotary evaporator, then extract with equal volumes of petroleum ether and ethyl acetate, evaporate the extract to dryness, and then use 70% ethanol Dissolve, and finally separate and extract with high-performance liquid chromatography to obtain apigenin;

[0050] 2) Mix the apigenin prepared in step 1) with Baitangping according to the formula to prepare a pharmaceutical composition. ...

Example Embodiment

[0061] Embodiment 3 pharmaceutical composition of the present invention is to the hypoglycemic test effect of diabetic model mice

[0062] 8 to 12-week-old db / db mice (provided by Shanghai Experimental Animal Center) with male insulin resistance against the background of C57Bl / 6 mice were selected as experimental mice.

[0063] We first administered protamine zinc recombinant human insulin mixed injection for 2 weeks,

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Abstract

The invention relates to the pharmaceutical technical field. The extraction method of apigenin comprises the steps of 1) enzymatically hydrolyzing celery which is taken as a substrate for 10 hours at 30-35 DEG C through a mixing solution of apigenin which is 33-37% and glucosidase which is 43-47% in mass percentage concentration; and 2) ultrasonically extracting enzymatic hydrolysate obtained from the step 1) through an ethanol solution of 70%, condensing an extracting solution, extracting respectively through petroleum ether and ethyl acetate, evaporating the extracting solution, dissolving through ethanol of 70%, and separating and extracting through a high performance liquid chromatograph. The pharmaceutical composition for treating diabetes consists of apigenin and glucobay at mass ratio of 1: (2-5). The extraction method, which extracts apigenin through a multi-enzymatic process, greatly improves extraction yield. The pharmaceutical composition disclosed by the invention not only can effectively control blood sugar but also can avoid circumstances of low blood sugar and rapid weight loss of a patient due to simple use of a diabetes medicine.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to a method for extracting apigenin, a pharmaceutical composition added with apigenin for treating diabetes and an application thereof. Background technique [0002] With the improvement of people's living standards, diabetes has become a global public health problem, and it has also caused direct problems to the health of our people. Diabetes itself can not only cause death, but also its complications such as foot rot, diabetic nephropathy, high blood pressure, and cardiovascular disease have expanded the harm of diabetes to all areas of the human body. Currently, there are 246 million people with diabetes worldwide, of which type 2 diabetes accounts for 90%. China currently estimates that there are 92.4 million people with diabetes over the age of 20, ranking among the highest in the world. Therefore, the treatment and prevention of diabetes has become an urgent problem. In ad...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40A61K31/352A61P3/10
CPCA61K31/352A61K31/7036C07D311/30C07D311/40A61K2300/00
Inventor 张谦
Owner 南通大学技术转移中心有限公司
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