Double chain adaptor, application thereof and method for constructing end-pairing DNA library

Abstract

The invention discloses a double chain adaptor, application thereof and a method for constructing end-pairing DNA library. The double chain adaptor comprises an adaptor A and an adaptor B, and the adaptor A and the adaptor B respectively comprise a LoxP sequence and an auxiliary sequence for linking the LoxP sequence to the two ends of a to-be-cyclized DNA fragment in a same direaction. By applying the technical scheme provided by the invention, based on the characteristics of the Cre recombinase/LoxP action system (when two LoxP sequences are disposed at one DNA chain and has the same direction, Cre recombinase is capable of effectively excising the sequence between the two LoxP sites, and the excised sequence automatically becomes annularity, so that the cyclization of a DNA fragment is realized), the double chain adaptor is designed and is efficiently linked to the two ends of a DNA fragment for constructing the end-pairing DNA library in a T-A clone manner, and the Cre recombinase is employed for efficiently enabling the DNA fragment for constructing the end-pairing DNA library to be cyclized.

Description

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AI Technical Summary

Benefits of technology

This technology allows for efficient creation of genetic material that includes specific parts called lox proteins or other factors like lignin from plants without damaging them during production process. By linking these elements together with certain chemicals, they become more stable when stored separately until needed later. These techniques allow scientists to study how different genes work alongside each others' own properties while also making it easier to make things up properly.

Problems solved by technology

Technological Problem addressed in this patents relates to improving the speed and precision of biomedical analysis while reducing costs associated therewith. Current techniques involve performing serial analyses that require multiple generations of starting material before generating new materials due to their time consumption and complexity. There have been attempts to develop faster and more efficient systems for studying these types of processes without sacrificially compiling them into smaller pieces.

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