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Method for Determining the Origin of Pulsatilla Pulsatillae Using Stable Isotope Ratio

A technology of stable isotope and pulsatilla, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve problems such as the seldom application of stable isotope mass spectrometry technology, complex sources of traditional Chinese medicine, and differences in the content of active ingredients, so as to solve the problem of origin Tracing the source, safeguarding the interests of consumers, and low cost

Inactive Publication Date: 2016-04-13
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the stable isotope mass spectrometry technology is rarely used in the identification of the origin of traditional Chinese medicine; moreover, the origin of traditional Chinese medicine is more complicated. A Chinese medicinal material often has several or even dozens of origins. The content is also different
In view of the particularity of the identification of the origin of traditional Chinese medicine, there is currently no specific method for identifying the origin of Pulsatilla pulsatillae by using stable isotope mass spectrometry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1.1 N%, C%, δ of Pulsatilla samples from the real place of origin 15 N and δ 13 C determination

[0025] 1) Real Pulsatilla samples were excavated from Heilongjiang, Liaoning, Anhui, Henan, Gansu and other places where Pulsatilla is mainly produced;

[0026] 2) Take the sample rhizome of Pulsatilla pulsatillae, air-dry and preserve;

[0027] 3) Before the determination of stable isotope mass spectrometry, take a small amount of sample and heat it in an oven at 60°C for 1 hour, then pulverize it after cooling, pass it through a 60-mesh sieve, and wait for the test;

[0028] 4) Wrap the sample in a tin foil boat, and use elemental analysis-stable isotope mass spectrometry for δ 15 N, δ 13 C, N%, C% are measured; The measurement results are as shown in Table 1:

[0029] Table 1

[0030] Numbering

[0031] It can be seen from Table 1 that the δ 15 N and δ 13 The difference in C value is significant; while the Pulsatilla 15 N and δ 13 C values ​​are rela...

Embodiment 2

[0033] 2.1 Construction of the δ15N and δ13C databases of Pulsatilla radiata from the real place of origin

[0034] 1) Take Psyllium pulsatilla samples from different known origins;

[0035] 2) Take the sample rhizome of Pulsatilla pulsatillae, air-dry and preserve;

[0036] 3) Before the determination of stable isotope mass spectrometry, take a small amount of sample and heat it in an oven at 60°C for 1 hour, after pulverization, pass through a 60-mesh sieve for testing;

[0037] 4) Wrap the sample in a tin foil boat, and use elemental analysis-stable isotope mass spectrometry for δ 15 N, δ 13 Determination of C, N%, and C%, to construct the true origin of Pulsatilla δ 15N and δ 13 C database; the database is shown in Table 2.

[0038] Table 2

[0039] Numbering

[0040] 2.2 Origin traceability of Pulsatilla grandis

[0041] 1) Purchase samples of Pulsatilla pulsatilla medicinal materials from the market;

[0042] 2) Before the determination of stable isoto...

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Abstract

The invention discloses a method for judging the origin of traditional Chinese medicine Pulsatilla pulsatillae by using stable isotope ratio, which comprises the steps of determining N%, C%, δ15N and δ13C values ​​in Pulsatilla pulsatilla samples by means of elemental analysis-stable isotope mass spectrometry. The main steps are as follows: by measuring the N%, C%, δ15N and δ13C values ​​in Pulsatilla samples from different origins, construct the N%, C%, δ15N and δ13C database of Pulsatilla samples from real origins; determine the N%, C %, δ15N and δ13C values; use statistical software to carry out Fisher discriminant analysis, and run the program to identify the true origin of samples of Pulsatilla pulsatilla samples from unknown origins. The present invention utilizes elemental analysis-stable isotope mass spectrometry to establish a method capable of accurately identifying the origin of Pulsatilla pulsatillae, solves the technical problems existing in the traceability of the origin of traditional Chinese medicines in my country, helps protect the interests of consumers, and promotes the healthy and orderly development of the Chinese medicine industry.

Description

technical field [0001] The invention belongs to the technical field of traceability of the origin of the traditional Chinese medicine Pulsatilla pulsatillae, and specifically relates to a method for determining the origin of the traditional Chinese medicine Pulsatilla pulsatillae by using the ratio of stable isotopes. Background technique [0002] Pulsatilla is a common species of Chinese medicinal materials with high medicinal value. It has the effects of clearing away heat and detoxification, cooling blood, improving eyesight, and stopping dysentery. , carbuncle sore, scrofula. Distributed in Liaoning, Hebei, Jilin, Henan, Anhui, Gansu and other places, the ones produced in Anhui were used as authentic medicinal materials in ancient times. Due to different natural conditions and ecological environments, the quality of medicinal materials varies from place to place. Authentic medicinal materials are better in quality and curative effect than those produced in other regions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
Inventor 张莉陈剑梁莉莉梁齐
Owner SHANGHAI JIAOTONG UNIV
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