Expression method of Beclin1 and MAP1-LC3 (microtubule-associated protein 1-light chain 3) in steroid-induced avascular necrosis of femoral head of rabbit

Abstract

The invention discloses an expression method of Beclin1 and MAP1-LC3 (microtubule-associated protein 1-light chain 3) in steroid-induced avascular necrosis of femoral head of a rabbit, detection of a bone cell apoptosis index of a femoral head tissue of a rabbit SANFH (steroid-induced avascular necrosis of femoral head) model and the MAP1-LC3 expression shows that autophagy-related gene Beclin1 can regulate autophagy activity and also can selectively combine with family members of Bcl-2 (B-cell lymphoma-2) to regulate the cell apoptosis activity. By detection of the expression of the Beclin1 in the SANFH model, the expression method provides a new molecular target for treatment of SANFH. The expression method is simple and convenient in operation, better solves the problem that no study on cell apoptosis and autophagy expression and mutual relation thereof in the SANFH model is found at home and abroad, and fills the blank of the study on cell apoptosis and autophagy expression and the mutual relation thereof in an ANFH (avascular necrosis of femoral head) model.

Description

technical field [0001] The invention belongs to the technical field of research on avascular necrosis of the femoral head, and in particular relates to a method for expressing Beclin1 and MAP1-LC3 in steroid-induced avascular necrosis of the femoral head in rabbits. Background technique [0002] Steroid-induced avascular necrosis of femoral head (Steroid-induced avascular necrosis of femoral head, SANFH) refers to the pathological process caused by the death of active components of the femoral head after long-term or short-term massive use of adrenal cortex hormones (hereinafter referred to as hormones). SANFH accounts for the first cause of non-traumatic avascular necrosis of the femoral head. Due to the wide application of hormones in clinical practice, the incidence of SANFH is increasing year by year. At present, there are many hypotheses about the pathogenesis of SANFH, including: increased intraosseous pressure, decreased bone perfusion, fat embolism, hypercoagulable ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide a method for the expression of Beclin1 and MAP1-LC3 in rabbit steroid-induced avascular necrosis of the femoral head, aiming to solve the problem of cell apoptosis and autophagy in the SANFH model that has not been seen at home and abroad. the expression of and the question of their interrelationships

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