Rooting induction, root promoting and seedling cultivation method of oil palm plantlets

A technology for induction medium and tissue culture seedlings, which is applied in the fields of rooting induction and root promotion of oil palm tissue culture seedlings, can solve the problems of poor population uniformity, difficulty in realizing clonal breeding, and difficulty in preserving the excellent characteristics of parents, etc. To achieve the effect of short hair root time, saving the amount of agar and growth regulator substances, and high hair root synchronization

Active Publication Date: 2014-04-02
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Oil palm breeding is mainly carried out through seeds, but because oil palm genes are generally heterozygous, it is difficult to preserve the genetic traits of the parents in their offspring, and the population uniformity is poor. Moreover, oil palm has only one growth point, which makes it difficult to realize traditional clonal breeding

Method used

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  • Rooting induction, root promoting and seedling cultivation method of oil palm plantlets
  • Rooting induction, root promoting and seedling cultivation method of oil palm plantlets

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Experimental program
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Effect test

Embodiment 1

[0019] 1. Preparation of rooting induction medium: WPM+NAA0.1mg.L -1 + Paclobutrazol 1mg.L -1 + sucrose 3g.L -1 +Agar 7g.L -1 +Activated carbon 1g.L -1 , pH5.6. Each test tube is filled with about 25-30ml of culture medium, and after being covered, the 3 , Sterilize under saturated steam pressure at 121°C for 20 minutes.

[0020] 2. Select 200 rootless seedlings regenerated from robust oil palm leaf-derived somatic embryos with a stem base diameter of 2 to 3 mm and a seedling height of 5 to 6 cm, remove part of the leaves and part of the base tissue of the stem, and transfer to step 1 under sterile conditions ) prepared in the rooting induction medium, culture conditions: temperature 28 ± 1 ℃, light intensity about 1000lx, light 12h per day. Cultured for 8 weeks. Recorded data: the number of rooted plants is 128, and the root length is 0.5-3 cm. The rooting induction rate is 64%.

[0021] 3. Preparation of root-promoting and seedling-promoting medium: WPM+sucrose 75g....

Embodiment 2

[0024] 1. Preparation of rooting induction medium: WPM+NAA1mg.L -1 + Paclobutrazol 1mg.L -1 + sucrose 60g.L -1 +Agar 7g.L -1 +Activated carbon 1g.L -1 , pH5.6. Each test tube is filled with about 25ml of culture medium, and after being covered, the 3 , Sterilize under saturated steam pressure at 121°C for 20 minutes.

[0025] 2. Select 200 rootless seedlings regenerated from robust oil palm leaf-derived somatic embryos with a diameter of 5-6 mm at the base of the stem and a height of 8-10 cm. After cutting off part of the leaves and part of the base tissue of the stem section, they are transferred to step 1 under sterile conditions. 1) Cultivate in the prepared rooting induction medium, culture conditions: temperature 26±1°C, light intensity about 1100lx, light 12h per day. Cultured for 10 weeks. Recorded data: the number of rooted plants is 184, and the root length is 0.5-3 cm. The rooting induction rate is 92%.

[0026] 3. Preparation of root-promoting and seedling-...

Embodiment 3

[0029] 1. Preparation of rooting induction medium: WPM+NAA5mg.L -1 + Paclobutrazol 1mg.L -1 + sucrose 60g.L -1 +Agar 7g.L -1 +Activated carbon 1g.L -1 , pH6.0. Each test tube is filled with about 25ml of culture medium, and after being covered, the 3 , Sterilize under saturated steam pressure at 121°C for 20 minutes.

[0030] 2. Select 200 rootless seedlings regenerated from robust oil palm leaf-derived somatic embryos with a diameter of 5-6 mm at the base of the stem and a height of 8-10 cm. After cutting off part of the leaves and part of the base tissue of the stem section, they are transferred to step 1 under sterile conditions. 1) Cultivate in the prepared rooting induction medium, culture conditions: temperature 27±2°C, light intensity about 900lx, light 12h per day. Cultured for 9 weeks. Recorded data: the number of rooted plants is 173, and the root length is 0.5-3cm. The rooting induction rate is 86.5%.

[0031] 3. Preparation of root-promoting and seedling-p...

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Abstract

The invention discloses a rooting induction, root promoting and seedling cultivation method of oil palm plantlets. The method comprises the following steps: carrying out conventional treatment on rootless plantlets of oil palm under a sterile condition, and then transferring to a rooting induction culture medium to cultivate; inducing a plant to form a root, and then transferring to a root promoting and seedling cultivation culture medium to carry out root promoting and seedling cultivation, so as to obtain a complete plant of the oil palm plantlets with a developed robust root. A set of rooting induction, root promoting and seedling cultivation technology of the oil palm plantlets, which is strong in maneuverability, and short in rooting and seedling cycle, and saves the cost is built by using different culture media and cultivation conditions, so as to obtain the developed robust root. The rooting induction, root promoting and seedling cultivation method can be applied to large-scale efficient production of the oil palm plantlets, a technical support is provided for production of high-quality oil palm plantlets, and development of high-efficiency commercialized production of the oil palm plantlets is facilitated. Therefore, the rooting induction, root promoting and seedling cultivation method has practical significance.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for rooting induction and root-promoting seedling cultivation, in particular to a method for rooting induction and root-promoting seedling cultivation of oil palm tissue culture seedlings. Background technique [0002] Oil palm (Elaeis guineensis Jacq), a perennial tree with a single growth point in the palm family (Palmae), is one of the important oil crops in the tropics and has the reputation of "the king of oil in the world". The oil palm oil yield is extremely high, and it can be used as edible oil, industrial raw materials, bio-energy, etc. It has a wide range of uses, has a large market demand and is favored, and the industry is developing rapidly. Therefore, tropical and subtropical regions around the world are competing to introduce and plant oil palms. [0003] Oil palm breeding is mainly carried out through seeds, but because oil palm genes are gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 潘登浪邹积鑫曾宪海林位夫姚行成张希财周立军王军刘钊李炜芳
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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