A kind of rooting induction of oil palm tissue culture seedlings and cultivation method for promoting roots and strong seedlings

A technology for inducing culture medium and tissue culture seedlings, which is applied in the field of oil palm tissue culture seedling rooting induction and root-promoting and strong seedling cultivation, can solve the problems of difficulty in preserving the excellent characteristics of parents, difficulty in realizing clonal breeding, poor population uniformity, and the like, To achieve the effect of saving the amount of agar and growth regulating substances, high synchronization of hair roots, and short hair root time

Active Publication Date: 2015-09-23
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Oil palm breeding is mainly carried out through seeds, but because oil palm genes are generally heterozygous, it is difficult to preserve the genetic traits of the parents in their offspring, and the population uniformity is poor. Moreover, oil palm has only one growth point, which makes it difficult to realize traditional clonal breeding

Method used

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  • A kind of rooting induction of oil palm tissue culture seedlings and cultivation method for promoting roots and strong seedlings
  • A kind of rooting induction of oil palm tissue culture seedlings and cultivation method for promoting roots and strong seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0019] 1. Preparation of rooting induction medium: WPM+NAA0.1mg.L -1 + Paclobutrazol 1mg.L -1 +Sucrose 3g.L -1 +Agar 7g.L -1 +Activated carbon 1g.L -1 , PH5.6. Each test tube is packed with about 25~30ml culture medium, after capping, at 1.2Kg / cm 3 , Sterilize at 121℃ under saturated steam pressure for 20min.

[0020] 2. Select strong oil palm leaf-derived somatic embryos with a stem base diameter of 2 to 3 mm and a seedling height of 5 to 6 cm to regenerate rootless seedlings. Part of the leaves and part of the base tissue of the stem are removed, and go to step 1 under aseptic conditions. ) Cultivation in the prepared rooting induction medium, culture conditions: temperature 28±1℃, light intensity about 1000lx, light for 12h per day. Cultivate for 8 weeks. Record data: 128 rooting plants, with root length of 0.5-3cm. The rooting induction rate is 64%.

[0021] 3. Preparation of medium for promoting roots and strong seedlings: WPM+sucrose 75g.L -1 , PH5.6. Each test tube is fi...

Embodiment 2

[0024] 1. Preparation of rooting induction medium: WPM+NAA1mg.L -1 + Paclobutrazol 1mg.L -1 +Sucrose 60g.L -1 +Agar 7g.L -1 +Activated carbon 1g.L -1 , PH5.6. Each test tube is filled with about 25ml of culture medium, after capping, at 1.2Kg / cm 3 , Sterilize at 121℃ under saturated steam pressure for 20min.

[0025] 2. Select strong oil palm leaf-derived somatic embryos with a stem base diameter of 5-6mm and a seedling height of 8-10cm to regenerate rootless seedlings. After cutting off part of the leaves and part of the stem base tissue, proceed to the steps respectively under aseptic conditions 1) Cultivate in the prepared rooting induction medium, the culture conditions: temperature 26±1℃, light intensity about 1100lx, light for 12h per day. Cultivate for 10 weeks. Record data: The number of rooting plants is 184, and the root length is 0.5-3cm. The rooting induction rate was 92%.

[0026] 3. Preparation of medium for promoting roots and strong seedlings: WPM+sucrose 75g.L -...

Embodiment 3

[0029] 1. Preparation of rooting induction medium: WPM+NAA5mg.L -1 + Paclobutrazol 1mg.L -1 +Sucrose 60g.L -1 +Agar 7g.L -1 +Activated carbon 1g.L -1 , PH6.0. Each test tube is filled with about 25ml of culture medium, after capping, at 1.2Kg / cm 3 , Sterilize at 121℃ under saturated steam pressure for 20min.

[0030] 2. Select strong oil palm leaf-derived somatic embryos with a stem base diameter of 5-6mm and a seedling height of 8-10cm to regenerate rootless seedlings. After cutting off part of the leaves and part of the stem base tissue, proceed to the steps respectively under aseptic conditions 1) Cultivate in the prepared rooting induction medium, the culture conditions: temperature 27±2℃, light intensity about 900lx, light for 12h per day. Cultivate for 9 weeks. Recorded data: The number of rooting plants was 173, and the root length was 0.5-3cm. The rooting induction rate was 86.5%.

[0031] 3. Preparation of medium for promoting roots and strong seedlings: WPM+sucrose 75g...

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Abstract

The invention discloses a rooting induction, root promoting and seedling cultivation method of oil palm plantlets. The method comprises the following steps: carrying out conventional treatment on rootless plantlets of oil palm under a sterile condition, and then transferring to a rooting induction culture medium to cultivate; inducing a plant to form a root, and then transferring to a root promoting and seedling cultivation culture medium to carry out root promoting and seedling cultivation, so as to obtain a complete plant of the oil palm plantlets with a developed robust root. A set of rooting induction, root promoting and seedling cultivation technology of the oil palm plantlets, which is strong in maneuverability, and short in rooting and seedling cycle, and saves the cost is built by using different culture media and cultivation conditions, so as to obtain the developed robust root. The rooting induction, root promoting and seedling cultivation method can be applied to large-scale efficient production of the oil palm plantlets, a technical support is provided for production of high-quality oil palm plantlets, and development of high-efficiency commercialized production of the oil palm plantlets is facilitated. Therefore, the rooting induction, root promoting and seedling cultivation method has practical significance.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a rooting induction and root-promoting strong seedling culture method, in particular a rooting induction and root-promoting strong seedling culture method of oil palm tissue culture seedlings. Background technique [0002] Oil palm (Elaeis guineensis Jacq), Palmae (Palmae) oil palm is a perennial single-growing tree, one of the important oil crops in the tropics, and is known as the "World Oil King". Oil palm has a very high oil production. It can be used as edible oil, industrial raw material, bio-energy, etc. It has a wide range of uses. The market demand is large and popular. The industry is developing rapidly. Therefore, tropical and subtropical regions around the world are competing to introduce oil palm for cultivation. [0003] Oil palm breeding is mainly carried out by seeds, but because oil palm genes are generally heterozygous, the genetic traits of its offspring ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 潘登浪邹积鑫曾宪海林位夫姚行成张希财周立军王军刘钊李炜芳
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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