Ultralow temperature cryopreservation and recovery method of Micropterus salmoides sperm

A technology of ultra-low temperature freezing and largemouth bass, which is applied to the preservation of human or animal bodies, germ cells, and vertebrate cells, etc. It can solve the problems of decreased resistance to disease, decreased growth rate, lack of directional breeding, etc., to the extent of damage Small size, short operation time, good induction effect

Active Publication Date: 2014-04-16
SUZHOU MUNICIPAL SHENHANG ECO TECH DEV CO LTD
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  • Summary
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AI Technical Summary

Problems solved by technology

However, due to the lack of directional selective breeding due to domestication of wild species at the time of introduction, largemouth bass have experienced varying degrees of germplasm degradation over time, mainly manifested in early sexual maturity age, reduced growth rate, and reduced disease resistance. , it is imperative to carry out the breeding of largemouth bass, and to breed strains with fast growth and good quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The choice of broodstock: the original species or improved species with normal shape, good growth, no disease, and reaching sexual maturity.

[0016] Sperm collection: During the breeding period of largemouth bass, 800 UI / kg was injected intraperitoneally to induce sperm enhancement. After 36 hours, the semen was collected and placed in a clean mortar pre-cooled in a refrigerator at 4°C.

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PUM

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Abstract

The invention provides an ultralow temperature cryopreservation and recovery method of Micropterus salmoides sperm, which comprises the following steps: (1) collecting seminal fluid, (2) selecting anti-freezing liquid, (3) diluting and balancing sperms, (4) refrigerating sperms, (5) unfreezing and recovering sperms. The method provided by the invention has the advantages of short operation time, good induction effect, little toxicity effect, little damage degree, high survival rate, etc., and has important theoretical meanings and application values for germplasm preservation, genetic breeding, sustainable culture and disease control and other aspects. The invention also provides a purpose of ultralow temperature cryopreservation and recovery method of Micropterus salmoides sperm.

Description

technical field [0001] The invention relates to the technical field of sperm freezing, in particular to a cryopreservation method for largemouth bass sperm. Background technique [0002] my country is a big aquaculture country. After the rapid development of conventional aquaculture species, the aquaculture of special aquatic products has developed rapidly. Largemouth bass has many advantages such as fast growth, strong adaptability, less disease, and easy fishing. Its meat is delicious and nutritious, and it is very popular among farmers and consumers. Since the introduction in the 1980s, large-scale industries have been formed in Guangdong, Zhejiang and Jiangsu. However, due to the lack of directional selective breeding due to domestication of wild species at the time of introduction, largemouth bass have experienced varying degrees of germplasm degradation over time, mainly manifested in early sexual maturity age, reduced growth rate, and reduced disease resistance. , I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/076
Inventor 徐海华王荣泉宣云峰
Owner SUZHOU MUNICIPAL SHENHANG ECO TECH DEV CO LTD
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