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Camellia saponin compound, preparation method and application thereof and anti-tumour drug prepared from same

An anti-tumor drug, the technology of Camellia oleifera saponins, applied in the field of anti-tumor drugs, can solve the problems of complex aglycon structure and different combination modes, etc.

Inactive Publication Date: 2014-04-30
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Camellia oleifera saponins are mostly oleanane-type pentacyclic triterpenoid saponins, including aglycone, sugar, and organic acid. However, the complex structure of aglycone, many types of sugar parts and different combinations have resulted in a large number of saponins in camellia oleifera. , which poses a challenge to the further purification and separation of camellia oleifera saponins
Therefore, although studies have found that the total saponin extract of camellia oleifera has anti-tumor activity, it is still unknown which monomer saponin plays the role, and in-depth research is needed

Method used

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  • Camellia saponin compound, preparation method and application thereof and anti-tumour drug prepared from same
  • Camellia saponin compound, preparation method and application thereof and anti-tumour drug prepared from same
  • Camellia saponin compound, preparation method and application thereof and anti-tumour drug prepared from same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1 has the preparation of the camellia saponin compound of structure shown in formula I

[0082] extract:

[0083] Take 2000g of dried Camellia oleifera root, crush it into sawdust with a grinder, add 16L of 70% ethanol aqueous solution by volume, soak at 40°C for 10h, heat to reflux, extract for 3.0h, collect the extract, filter the extract, Concentrated under reduced pressure to obtain 632 g of liquid extract.

[0084] Macroporous resin enrichment:

[0085] Take the liquid extract obtained in the extraction step, add 6.0L of water, stir to dissolve, filter with a 100-mesh filter cloth, and centrifuge the filtrate at 12000r / min for 10min. After centrifugation, 6.2L of supernatant is obtained. Pore ​​resin was further separated, and eluted with 10L water, 15L 30% ethanol aqueous solution, and 15L 70% ethanol aqueous solution successively, collected 70% ethanol aqueous solution eluted fraction, concentrated under reduced pressure, after testing, the obtained ...

Embodiment 2

[0110] Embodiment 2 has the preparation of the camellia saponin compound of structure shown in formula I

[0111] extract:

[0112] Take 2000g of dried Camellia oleifera root, crush it into sawdust with a pulverizer, add 40L of 0.1% ethanol aqueous solution by volume, soak at 50°C for 12h, heat to reflux, extract three times, each time for 0.5h, collect all the extracts, The extract was filtered and concentrated under reduced pressure to obtain 618 g of liquid extract.

[0113] Macroporous resin enrichment:

[0114] Take the liquid extract obtained in the extraction step, add 12.36L of water, stir to dissolve, filter with a 300-mesh filter cloth, and centrifuge the filtrate at 12000r / min for 10min. After centrifugation, 6.0L of supernatant is obtained. Pore ​​resin was further separated, and eluted successively with 10L water, 15L 60% ethanol aqueous solution, and 10L 95% ethanol aqueous solution, collected 95% ethanol aqueous solution eluted components, and concentrated und...

Embodiment 3

[0125] Embodiment 3 has the preparation of the camellia saponin compound of structure shown in formula I

[0126] extract:

[0127] Take 2000g of dry Camellia oleifera root, crush it into sawdust with a pulverizer, add 10.0L of 95% by volume ethanol aqueous solution, soak for 8h at 30°C, heat to reflux, extract twice, each time for 1.5h, collect all the extracted liquid, the extract was filtered and concentrated under reduced pressure to obtain 572 g of liquid extract.

[0128] Macroporous resin enrichment:

[0129] Take the liquid extract obtained in the extraction step, add 2.86L of water, stir to dissolve, filter with a 200-mesh filter cloth, centrifuge the filtrate at 12000r / min for 10min, after centrifugation, obtain 5.5L of supernatant, take the supernatant through D101 Pore ​​resin was further separated, and eluted successively with 10L water, 15L 40% ethanol aqueous solution, and 15L 75% ethanol aqueous solution, collected 75% ethanol aqueous solution eluted componen...

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Abstract

The invention belongs to the technical field of pharmacochemistry and discloses a camellia saponin compound, a preparation method and application thereof and an anti-tumour drug prepared from the same. The oil-tea camellia saponin chemical compound adopts the structure shown in the formula I, has remarkable effects on inhibiting liver cancer cell, breast cancer cell, melanoma cell and lung cancer cell, and can effectively inhibit the growth of liver tumour, so that the oil-tea camellia saponin chemical compound has remarkable tumour resisting activity and can be used for the preparation of anti-tumour drugs.(The formula I is shown in the specification).

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry, and in particular relates to a camellia oleifera saponin compound, its preparation method, application and antitumor drug prepared therefrom. Background technique [0002] Camellia oleifera (Camellia oleifera Abel), also known as tea seed tree, camellia oil tree, white flower tea, is an evergreen small tree of the family Theaceae (Theaceae) Camellia Linn, mainly distributed in tropical and subtropical regions. In my country, camellia oleifera is produced in the southwest and southeast regions, covering 17 provinces. Camellia oleifera is an important woody edible oil tree species, and it is called "the world's four largest woody oil tree species" together with olive, oil palm and coconut. Camellia oleifera oil is obtained by pressing oil from the seeds of camellia oleifera. The unsaturated fatty acid content of tea oil is as high as 90%, which is much higher than that of rapeseed oil,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P35/00
Inventor 许琼明杨世林李夏李笑然刘艳丽陈重
Owner SUZHOU UNIV
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