Preparation and detection method for ELISA kit detecting Fumonisins
A fumonisin and detection method technology, applied in the field of detection, can solve problems such as backward development, and achieve the effects of simple operation and sensitive detection
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[0032] An ELISA kit for detecting fumonisins, which includes an enzyme-labeled plate, fumonisins standard, fumonisin antibody working solution, fumonisin enzyme-labeled secondary antibody working solution, substrate solution A, substrate solution B, and stop solution And concentrated washing liquid.
[0033] The following specifically describes the preparation of the ELISA kit for detecting fumonisins in the present invention.
[0034] Preparation of Fumonisin Protein Conjugate:
[0035] The fumonisin hapten and the carrier protein BSA were mixed in 0.05 mol / L pH 9.6 carbonate buffer (CBS) at a binding ratio of 12:1, then added to carbodiimide, stirred for 1-2h, set Reaction at room temperature for 24 hours, and finally dialysis with 0.2 mol / L pH 7.6 PBS for two days to remove the unreacted hapten, and the fumonisin protein conjugate can be obtained.
[0036] Preparation of fumonisin antibody:
[0037] Three-month-old healthy white rabbits were selected for three immunizations. The do...
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