A kind of algae secondary cultivation device and cultivation method thereof
A technology of secondary culture and culture method, which is applied in the directions of single-cell algae, biochemical cleaning devices, microorganism-based methods, etc., to achieve the effect of convenient subculture, reduced workload, and small size
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] In this embodiment, Figure 1 ~ Figure 4 The reference signs correspond to the following: 1 perforated movable cover plate; 2 reflective device; 3 light source loading device; 4 gas loading device; 5 culture container; 6 movable pulley; 7 light source; 8 fixed ring; 9 "Z" type reflector; 10 Air filter valve; 11 Air volume control valve; 12 Inlet pipe; 13 Outlet valve; 14. Cotton plug or silicone plug; 15. Scale; 16. Short side of support plate; 17, Long side of support plate; 18. Stainless steel Column; 19, thin column; 20, vertical steel frame.
[0033] Such as figure 1 As shown, this embodiment discloses a secondary algae cultivation device, which includes a bottom support plate, and a reflector 2 is fixed on the bottom support plate to form a frame. The support plate is preferably stainless steel; a movable cover plate with holes mounted on the reflector 2 1. The perforated movable cover just covers the frame of the reflector; the perforated movable cover is provided w...
Embodiment 2
[0039] A method for culturing common algae using the above-mentioned device, the method includes the following steps:
[0040] A. Pour 2L of culture solution into the culture vessel under indoor conditions of 25±2℃. The culture solution can be tap water, culture medium or other water. The culture container and water are selectively sterilized according to the culture target. Inoculate algae into the culture vessel to a concentration of 10 4 Pcs / mL, the algae used for inoculation has no pollution and reaches a stable logarithmic growth phase.
[0041] B. The culture container is fixed in the algae cultivation device, the light source loading device and the gas loading device are turned on, and the algae density is observed after 4 days of continuous operation. During this process, the light source loading device operates according to the ratio of light to dark time of 16:8. The gas loading device, the loading gas volume is to form a series of bubbles.
[0042] C. Take a small amount...
Embodiment 3
[0043] Example 3: Cultivation of 5 common algae species
[0044] Algae is the most important primary producer in the water ecosystem and the main supplier of dissolved oxygen in the water. Algae are the direct or indirect food for fish and other economic animals, and are of great significance in determining the performance of waters. Researchers are focusing more and more on algae, and the expansion of indoor secondary cultivation is becoming more and more important. The use of a two-stage expansion culture device can easily obtain a pure culture solution of multiple algae species to meet the needs of research workers. In this study, five kinds of algae were cultivated to find out the growth status of the algae under the device.
[0045] 1 Culture materials
[0046] Chlamydomonas reinhardtii (Chlamydomonas reinhardtii), Selenastrum capricornutum (Selenastrum capricornutum), Chlorella pyrenoidosa (Chlorella pyrenoidosa), Scenedesmus obliquus (Scenedesmus obliquus), Chlorella vulgari...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More