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Ex-vitro rooting method for tissue culture blueberry seedlings

A tissue culture seedling and exogenous rooting technology is applied in the field of exogenous rooting of blueberry tissue culture seedlings. simple effect

Inactive Publication Date: 2014-06-25
HANGZHOU YOUGUO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for rooting blueberry tissue cultured seedlings outside the bottle, which mainly solves the technical problems of blueberry tissue cultured seedlings rooting outside the bottle, such as many training procedures, low survival rate, and high cost of seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: a kind of blueberry tissue culture seedling bottle rooting method of this example, its steps are:

[0022] a. Select well-growing blueberry tissue culture seedlings (Lanyuan), with a height of about 10-12 cm, strong branches, and domesticate them;

[0023] b. First place the blueberry tissue culture seedlings in the seedling hardening room. The seedling hardening room is dominated by scattered light, the light intensity is 3000lx-4000lx, and the temperature is controlled at 15°C-25°C. Continue to cultivate for 2-3 days;

[0024] c. Take the seedlings out of the bottle and cut each plant into 3 sections. The scissors must be strictly sterilized, and the rooting water should be dipped before cutting. The cutting depth should be about 1 cm. The cutting substrate should be moss and treated with 800 times carbendazim Disinfect, use after soaking for one day, wash the soaked moss with clean water, wring it dry and chop it up, and spread it on the seedling tr...

Embodiment 2

[0027] Embodiment 2: a kind of blueberry tissue culture seedling bottle rooting method of this example, its steps are:

[0028] a. Select well-growing blueberry tissue culture seedlings (Legexi), with a height of about 10-12 cm and strong branches, for hardening and domestication;

[0029] b. First place the blueberry tissue culture seedlings in the seedling hardening room. The seedling hardening room is dominated by scattered light, the light intensity is 3000lx-4000lx, and the temperature is controlled at 15°C-25°C. Continue to cultivate for 2-3 days;

[0030] c. Take the seedlings out of the bottle and cut each plant into 3 sections. The scissors must be strictly sterilized, and the rooting water should be dipped before cutting. The cutting depth should be about 1 cm. The cutting substrate should be moss and treated with 800 times carbendazim Disinfect, use after soaking for one day, wash the soaked moss with clean water, wring it dry and chop it up, and spread it on th...

Embodiment 3

[0033] Embodiment 3: a kind of blueberry tissue culture seedling rooting method outside the bottle of this example, its steps are:

[0034] a. Select well-growing blueberry tissue culture seedlings (O'Neill), with a height of about 10-12 cm, strong branches, and domesticate them;

[0035] b. First place the blueberry tissue culture seedlings in the seedling hardening room. The seedling hardening room is dominated by scattered light, the light intensity is 3000lx-4000lx, and the temperature is controlled at 15°C-25°C. Continue to cultivate for 2-3 days;

[0036] c. Take the seedlings out of the bottle and cut each plant into 3 sections. The scissors must be strictly sterilized, and the rooting water should be dipped before cutting. The cutting depth should be about 1 cm. The cutting substrate should be moss and treated with 800 times carbendazim Disinfect, use after soaking for one day, wash the soaked moss with clean water, wring it dry and chop it up, and spread it on the...

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Abstract

The invention relates to a blueberry culture method, in particular to an ex-vitro rooting method for tissue culture blueberry seedlings. The ex-vitro rooting method includes that tissue culture blueberry seedlings which grow strongly are selected and are exercised domesticated; mainly scattered light is emitted in seedling exercising chambers; the seedlings are fetched out from bottles, each seedling is pruned to obtain 2-4 sections, the sections are dipped in rooting liquid before cutting is carried out, moss is sterilized and treated, and is soaked in water one day before being used, and the soaked moss is washed by clear water, is wrung and minced and then is spread on seedling growing discs to obtain cutting media; water is sprayed on the seedlings every now and then in cutting procedures; the seedlings are sufficiently watered after cutting is completed, the environment humidity is controlled and ranges from 80% to 90%, the seedlings are watered every three other days, leaf fertilizers are sprayed on the seedlings once every two other weeks, bactericides are sprayed on the seedlings once every month, and robust blueberry moss seedlings can be acquired after four months, are transplanted into nutrition pots and are planted in the nutrition pots. The ex-vitro rooting method has the advantage that technical problems of large quantities of culture programs, low survival rate, high seedling cost and the like of an existing ex-vitro rooting method for tissue culture blueberry seedlings mainly can be solved.

Description

technical field [0001] The invention relates to a method for cultivating blueberries, in particular to a method for rooting blueberry tissue culture seedlings outside a bottle. Background technique [0002] Blueberry, also known as lingonberry and blueberry, belongs to the plant of the genus Vaccinium in the family Rhododendronaceae. It is a perennial deciduous or evergreen shrub or small shrub. Blueberry fruit contains various physiologically active ingredients such as anthocyanins and flavonoids, which can prevent brain aging, enhance heart function, improve eyesight and fight cancer. Therefore, it is listed as one of the five health foods for human beings by the International Food and Agriculture Organization. Blueberry will become the most promising fruit tree species in the world in this century. As one of the most important methods in asexual reproduction, cuttings are widely used in plant seedling breeding because of their simplicity, short seedling cycle, high repr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G1/00
Inventor 杨小兵
Owner HANGZHOU YOUGUO BIO TECH
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