Method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide

A technology of nano-copper oxide and fenoxyben, applied in the field of biomedicine, can solve problems such as the toxicity of nano-metal particles, and achieve the effects of improving curative effect, high bacteriostatic effect, and reducing dosage.

Inactive Publication Date: 2014-07-23
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nano metal particles themselves have certain toxicity to human cells, which limits their application to a certain extent.

Method used

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  • Method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide
  • Method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide
  • Method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide

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Experimental program
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Effect test

Embodiment 1

[0019] In the first step, the commercially available nano-copper oxide particles are dispersed in sterile water to make concentrations of 25, 50, and 200 mg l -1 solution, put it under ultrasound for 30 minutes to make it fully dispersed, dissolve cephalexin powder in sterile water to make a solution, the concentration is 4, 8mg l -1 ; the concentration will be 8 mg l -1 Cephalexin with a concentration of 50 mg l -1 Nano-copper oxide mixed with ultrasound for 30 minutes to make it fully effective.

[0020] In the second step, Escherichia coli ( BH21 ) was added to the prepared LB liquid medium, and cultured at 37°C for 24 hours to restore its activity. Dilute the cultured Escherichia coli liquid with LB medium to make its concentration reach 2.5×10 2 CFU. Take 2ml diluted Escherichia coli liquid and add 4, 8mg l -1 Cefalexin, 25, 50, 200 mg l -1 Nano copper oxide, 8 mg l -1 Cefalexin +50mg l -1 Nano-copper oxide mixed solution. Put the mixed solution into a constant...

Embodiment 2

[0023] In the first step, the commercially available nano-copper oxide particles are dispersed in sterile water to make concentrations of 25, 50, and 200 mg l -1 solution, put it under ultrasound for 30 minutes to fully disperse it, and dissolve the cephalexin powder in sterile water to make a solution (B), with concentrations of 4 and 8mg l -1 ; the concentration will be 8 mg l -1 Cephalexin with a concentration of 25 mg l -1 Nano-copper oxide mixed with ultrasound for 30 minutes to make it fully effective.

[0024] In the second step, Escherichia coli ( BH21 ) was added to the prepared LB liquid medium, and cultured at 37°C for 24 hours to restore its activity. Dilute the cultured Escherichia coli liquid with LB medium to make its concentration reach 2.5×10 2 CFU. Take 2ml diluted Escherichia coli liquid and add 4, 8mg l -1 Cefalexin, 25, 50, 200 mg l -1 Nano copper oxide, 8 mg l -1 Cefalexin +25mg l -1 Nano-copper oxide mixed solution. Put the mixed solution into...

Embodiment 3

[0027] In the first step, the commercially available nano-copper oxide particles are dispersed in sterile water to make concentrations of 25, 50, and 200 mg l -1 solution, put it under ultrasound for 30 minutes to fully disperse it, and dissolve the cephalexin powder in sterile water to make a solution (B), with concentrations of 4 and 8mg l -1 ; to a concentration of 4 mg l -1 Cephalexin with a concentration of 50 mg l -1 Nano-copper oxide mixed with ultrasound for 30 minutes to make it fully effective.

[0028] In the second step, Escherichia coli ( BH21 ) was added to the prepared LB liquid medium, and cultured at 37°C for 24 hours to restore its activity. Dilute the cultured Escherichia coli liquid with LB medium to make its concentration reach 2.5×10 2 CFU. Take 2ml diluted Escherichia coli liquid and add 4, 8mg l -1 Cefalexin, 25, 50, 200 mg l -1 Nano copper oxide, 4 mg l -1 Cefalexin +50mg l -1 Nano-copper oxide mixed solution. Put the mixed solution into a c...

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Abstract

The invention specifically relates to a method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide, which belongs to the technical field of biological medicine. According to the method, a nanometer copper oxide inorganic nano-material is added into cephalexin so as to improve antibacterial activity on Escherichia coli. According to results of antibacterial experiments, it is found that nanometer copper oxide has a good synergistic effect on antibacterial activity of cephalexin; drug effects produced in combined usage of nanometer copper oxide and cephalexin are far better than drug effects produced in separate usage of nanometer copper oxide and cephalexin. The method has the advantages of easy availability of raw materials, low cost and simple operation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for synergizing the antibacterial performance of cephalexin by nano-copper oxide. Background technique [0002] For a long time, people have been looking for antibiotic drugs with higher efficacy. At present, there are two main ways to improve the efficacy of drugs: one way is to develop new and powerful antibiotics, but because widespread use will induce drug resistance in pathogenic bacteria, which will inevitably lead to a reduction in drug efficacy, the other way is to develop new and powerful antibiotics. One method is to increase the dose of the drug to compensate for the decline in the efficacy of the drug. However, because doctors cannot accurately judge the end point of medication, the principle of overdose is always adopted in the process of medication treatment, and this will bring multiple burdens to patients physically, psychologically and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/34A61P31/04A61K31/545
CPCY02A50/30
Inventor 徐翔吴庆生
Owner TONGJI UNIV
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