Method for identifying whether blood-glucose-lowering traditional Chinese medicine contains pioglitazone or rosiglitazone or not

A technology for pioglitazone and traditional Chinese medicine, applied in the field of medical analysis, can solve the problems of harsh experimental conditions, inability to perform identification, and high detection cost, and achieve the effects of convenient operation, accurate and reliable identification results, and strong characteristics.

Active Publication Date: 2014-07-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the TLC method is simple and fast, it can only make a rough judgment on whether the hypoglycemic drug is adulterated, and cannot identify the specific ingredients in the adulterated hypoglycemic traditional Chinese medicine. show false positive or false negative results due to the influence of
Although HPLC and LC-MS can judge whether the adulterated hypoglycemic traditional Chinese medicine is specifically doped with pioglitazone, rosiglitazone or a mixture of the two, the analysis period is long and the experimental conditions are harsh, requiring specific chromatographic columns and flow equalizers. , the detection cost is high, especially when the drug regulatory department needs to detect and judge whether pioglitazone hydrochloride or rosiglitazone maleate or both are added to the hypoglycemic traditional Chinese medicine on site, these methods will be difficult to apply

Method used

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  • Method for identifying whether blood-glucose-lowering traditional Chinese medicine contains pioglitazone or rosiglitazone or not
  • Method for identifying whether blood-glucose-lowering traditional Chinese medicine contains pioglitazone or rosiglitazone or not
  • Method for identifying whether blood-glucose-lowering traditional Chinese medicine contains pioglitazone or rosiglitazone or not

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Instruments and samples

[0056] (1) Instrument: BWS415-785H portable Raman spectrometer (Bida Tech, USA), excitation wavelength 785nm;

[0057] (2) Preparation of silver sol: take silver nitrate (AgNO 3 ) 17mg was dissolved in 100ml of secondary deionized water, 1.6ml of 1% trisodium citrate was added, heated in a microwave oven on high heat for 6min, the solution was grayish green, cooled to room temperature, added water to the mark, and set aside.

[0058] (3) Preparation of the hypoglycemic traditional Chinese medicine solution to be tested and the reference substance solution: take a certain batch of hypoglycemic traditional Chinese medicine produced by A Pharmaceutical Factory as the hypoglycemic traditional Chinese medicine to be tested, and take the hypoglycemic traditional Chinese medicine to be tested, pioglitazone hydrochloride standard product, and horse An appropriate amount of rosiglitazone toic acid standard substance was prepared into methanol soluti...

Embodiment 2

[0085] 1. Instruments and samples

[0086] (1) Instrument: BWS415-785H portable Raman spectrometer (Bida Tech, USA), excitation wavelength 785nm;

[0087] (2) Preparation of silver sol: take silver nitrate (AgNO 3 ) 17mg was dissolved in 100ml of secondary deionized water, 1.6ml of 1% trisodium citrate was added, heated in a microwave oven on high heat for 6min, the solution was grayish green, cooled to room temperature, added water to the mark, and set aside.

[0088] (3) Preparation of hypoglycemic traditional Chinese medicine solution to be checked and reference substance solution: get the hypoglycemic traditional Chinese medicine of certain batch production of B pharmaceutical factory as the hypoglycemic Chinese medicine to be checked, make the methanol (analysis pure) solution.

[0089] 2. Testing the hypoglycemic traditional Chinese medicines to be tested

[0090] Figure 8 It is a flow chart of the technical solution of the present invention in Embodiment 2.

[009...

Embodiment 3

[0103] 1. Instruments and samples

[0104] (1) Instrument: BWS415-785H portable Raman spectrometer (Bida Tech, USA), excitation wavelength 785nm;

[0105] (2) Preparation of silver sol: take silver nitrate (AgNO 3 ) 17mg was dissolved in 100ml of secondary deionized water, 1.6ml of 1% trisodium citrate was added, heated in a microwave oven on high heat for 6min, the solution was grayish green, cooled to room temperature, added water to the mark, and set aside.

[0106] (3) Preparation of the hypoglycemic traditional Chinese medicine solution to be checked and the reference substance solution: get a certain batch of hypoglycemic Chinese medicine produced by C Pharmaceutical Factory as the hypoglycemic Chinese medicine to be checked, and make 1mol / L of methanol (analysis pure) solution.

[0107] 2. Testing the hypoglycemic traditional Chinese medicines to be tested

[0108] In Example 3, 1 μl of the methanol solution of the hypoglycemic traditional Chinese medicine to be test...

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Abstract

The invention provides a method for identifying whether a blood-glucose-lowering traditional Chinese medicine contains pioglitazone or rosiglitazone or not. The method comprises the following steps: carrying out thin layer chromatography on a blood-glucose-lowering traditional Chinese medicine to be detected; finding a suspected spot at a corresponding Rf value part of the pioglitazone and the rosiglitazone. The method is characterized by further comprising the following three steps: 1, collecting a series of one-dimensional surface enhanced Raman spectrums of the suspected spot to obtain a dynamic spectrum pattern of the blood-glucose-lowering traditional Chinese medicine to be detected; 2, pre-treating the dynamic spectrum pattern and drawing to obtain a two-dimensional correlation surface enhanced Raman spectrum of the blood-glucose-lowering traditional Chinese medicine to be detected; 3, judging whether the blood-glucose-lowering traditional Chinese medicine contains the pioglitazone or the rosiglitazone or not according to the two-dimensional correlation surface enhanced Raman spectrum of the blood-glucose-lowering traditional Chinese medicine to be detected. According to the technical scheme provided by the invention, whether the blood-glucose-lowering traditional Chinese medicine contains the pioglitazone or the rosiglitazone or not can be identified rapidly; the detection result is accurate and reliable, the cost is low and the operation is convenient.

Description

technical field [0001] The invention relates to a method for identifying whether pioglitazone or rosiglitazone is added to a hypoglycemic traditional Chinese medicine, and belongs to the technical field of medical analysis. Background technique [0002] In recent years, in order to seek high profits, some pharmaceutical companies have added low-cost and equivalent hypoglycemic western medicines pioglitazone hydrochloride, rosiglitazone maleate or a mixture of the two to traditional Chinese medicines. Can play a hypoglycemic effect, but the toxicity is relatively high, especially rosiglitazone maleate, because it will greatly increase the risk of heart attacks and strokes of users, the US Food and Drug Administration has issued a report in 2011 This product is completely removed from the shelves. If patients take these hypoglycemic western medicines and traditional Chinese medicines for a long time, it will not only cause various adverse reactions such as hypoglycemia, but e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 陆峰朱青霞李皓柴逸峰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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