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Preparation method of apigenin-7-O-beta-D-glucuronide, and use of apigenin-7-O-beta-D-glucuronide

A technology of glucuronide and apigenin, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problem of not finding the extraction of apigenin-7-o-β-D-glucuronide. methods, etc., to achieve simple operation and good effect.

Inactive Publication Date: 2014-08-06
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to analyze the pharmacological effects of the ingredients in Kudiezi, it is necessary to extract Kudiezi and further study the individual pharmacological effects of each ingredient for more accurate clinical application. At present, no apigenin-7-o-β- The extraction method of D-glucuronide and the literature on treating diseases

Method used

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  • Preparation method of apigenin-7-O-beta-D-glucuronide, and use of apigenin-7-O-beta-D-glucuronide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Apigenin-7-o-β-D-glucuronide preparation method, comprises the following steps:

[0097] Extraction: Weigh 10 kg of Kudiezi medicinal material, add 10 times the amount of 40% ethanol to the Kudiezi medicinal material, soak for 1 hour, reflux extraction twice, each time for 2 hours, combine the extracts, concentrate under reduced pressure at 60°C to obtain a concentration of 1.2 g / ml Concentrate Ⅰ;

[0098] Water precipitation: Add 6 times the amount of deionized water to the concentrated solution Ⅰ, dilute the concentration of the concentrated solution Ⅰ to 0.2g / ml, refrigerate at 4°C for 24 hours, filter, and use the filtrate for later use;

[0099]Purification: First, use macroporous adsorption resin I to purify, weigh 6.67kg of HZ820 macroporous adsorption resin according to the mass ratio of resin to crude drug of 1:1.5, pack the wet resin into a column, adjust the filtrate to pH3.5 with hydrochloric acid, and The filtrate was loaded with a sample concentration of ...

Embodiment 2

[0104] Apigenin-7-o-β-D-glucuronide preparation method, comprises the following steps:

[0105] Extraction: Weigh 20kg of Kudiezi medicinal material, add 15 times the amount of 70% ethanol to the Kudiezi medicinal material, soak for 3 hours, reflux extraction twice, each time for 2 hours, combine the extracts, concentrate under reduced pressure at 40°C to obtain a concentration of 0.8 g / ml Concentrate Ⅰ;

[0106] Water precipitation: add 3 times the amount of deionized water to the concentrated solution Ⅰ, dilute the concentration of the concentrated solution Ⅰ to 0.2g / ml, refrigerate at 10°C for 20h, filter, and use the filtrate for later use;

[0107] Purification: First, use macroporous adsorption resin I to purify. According to the mass ratio of resin to crude drug of 1:1.5, weigh 13.4kg of HZ820 macroporous adsorption resin, pack the wet resin into a column, and adjust the filtrate to pH 4.0 with hydrochloric acid. Load the filtrate with a sample concentration of 0.2g / ml...

Embodiment 3

[0112] Apigenin-7-o-β-D-glucuronide preparation method, comprises the following steps:

[0113] Extraction: Weigh 20kg of Kudiezi medicinal material, add 12 times the amount of 50% ethanol to the Kudiezi medicinal material, soak for 2 hours, reflux extraction twice, each time for 2 hours, combine the extracts, concentrate under reduced pressure at 50°C to obtain a concentration of 1.0 g / ml Concentrate Ⅰ;

[0114] Water precipitation: Add 4 times the amount of deionized water to the concentrated solution Ⅰ, dilute the concentration of the concentrated solution Ⅰ to 0.25g / ml, refrigerate at 6°C for 15 hours, filter, and use the filtrate for later use;

[0115] Purification: First, use macroporous adsorption resin I to purify. According to the mass ratio of resin to crude drug of 1:1.5, weigh 13.4kg of HZ820 macroporous adsorption resin, pack the wet resin into a column, and adjust the filtrate to pH 4.0 with hydrochloric acid. Load the filtrate with a sample concentration of 0....

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Abstract

The invention discloses a preparation method of apigenin-7-O-beta-D-glucuronide, and a use of of apigenin-7-O-beta-D-glucuronide, and belongs to the fields of extraction and application of effective components of traditional Chinese medicines. The method comprises the steps of extraction, water precipitation and purification. The method is simple and convenient, and the prepared apigenin-7-O-beta-D-glucuronide has a high purity, and has a good curative effect on the coronary heart disease and myocardial infarction.

Description

technical field [0001] The invention belongs to the extraction method and application of active ingredients of traditional Chinese medicine, in particular to the method and application of extracting flavonoid glycosides from Kudiezi. Background technique [0002] Kudiezi tastes pungent, bitter, and slightly cold, and has the effects of clearing heat and detoxifying, eliminating carbuncle and draining pus, removing blood stasis and relieving pain. The main components of Kudiezi are flavonoids, phenolic acids, sesquiterpene lactones, and triterpenoids, with a wide range of pharmacological effects: (1) Heart and cerebrovascular protection; it can increase coronary flow, reduce oxygen metabolism, improve Hypoxia tolerance, therapeutic effect on experimental myocardial infarction, increase cerebral blood flow, improve microcirculation, and vasoconstriction. (2) Effect on the blood system; it has anti-platelet aggregation, enhanced fibrinase activity, lowering blood fat, promotin...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P9/10
Inventor 姜新刚贾继明赵韶华唐思文王贵金裴彩云
Owner HEBEI YILING MEDICINE INST
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