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Application of classically activated macrophages in treatment of liver fibrosis

A technology of macrophages and liver fibrosis, which is applied in the field of treatment of liver fibrosis, can solve the problems of controversial treatment effects, achieve good therapeutic effects, accelerate degradation, and reduce the effects of liver fibrosis

Inactive Publication Date: 2014-08-13
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bone marrow cells are a group of heterogeneous cells, and which cellular components have a therapeutic effect on liver fibrosis is controversial

Method used

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  • Application of classically activated macrophages in treatment of liver fibrosis
  • Application of classically activated macrophages in treatment of liver fibrosis
  • Application of classically activated macrophages in treatment of liver fibrosis

Examples

Experimental program
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Embodiment Construction

[0023] 1. Culture and polarization induction of mouse bone marrow-derived macrophages

[0024] Healthy adult male C57 / B6 mice were selected, and the femur and tibia were completely removed. The marrow cavity of the long bone was washed with 1×PBS to obtain the cells, and the red blood cells were removed with the red blood cell lysate by repeated gentle pipetting. Obtain a single cell suspension, seed evenly into a 10cm dish, and culture overnight (preferably 16h) with α-MEM medium containing 10% fetal bovine serum to remove stromal cells that adhere faster, such as fibroblasts. The next day, collect non-adherent cells, evenly plant them in a culture dish, culture them with α-MEM medium containing 10% fetal bovine serum, add GM-CSF to make the final concentration 40ng / ml, and stimulate the bone marrow cells to Nuclear macrophage differentiation. On the 4th day of culture, half of the medium was changed, and GM-CSF was supplemented. Macrophages can be successfully induced af...

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Abstract

The invention relates to application of classically activated macrophages in treatment of liver fibrosis. The invention is characterized by application of the classically activated macrophages in treatment of liver fibrosis and preparation of drugs used for treating liver fibrosis. According to the invention, matrix metalloproteinase (MMPs) molecules highly expressed in M1 macrophages and a great number of MMPs secreted by the M1 macrophages and autologous macrophages entering the liver cooperate with each other to accelerate degradation of collagen fibers and mitigate liver fibrosis, wherein autologous macrophages are raised after reinfusion of the M1 macrophages. The M1 macrophages have a good therapeutic effect on liver fibrosis, so a novel effective method is provided for clinical treatment of liver fibrosis and cirrhosis.

Description

technical field [0001] The invention belongs to the field of liver fibrosis treatment and relates to the fields of macrophage polarization and cell therapy. It specifically relates to the application of a classic activated macrophage in the treatment of liver fibrosis. Background technique [0002] Liver fibrosis is caused by various persistent chronic liver injuries, and its gradual development can eventually lead to liver cirrhosis and even liver cancer, which seriously threatens people's life and health. Effective treatments for advanced cirrhosis are limited, but effective treatments can reverse liver fibrosis and even restore normal liver structure and function. Therefore, it is of great significance to explore effective methods for the treatment of liver fibrosis. Cell therapy for liver fibrosis has important clinical application prospects. At present, many scholars at home and abroad are studying the therapeutic effect of cell transplantation on liver fibrosis, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P1/16
Inventor 秦鸿雁马鹏飞窦科峰韩骅
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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