Breeding method of hybrid rice two-line sterile line with leaves carrying white midribs

Abstract

The invention discloses a breeding method of a hybrid rice two-line sterile line with leaves carrying white midribs. The breeding method comprises the following steps: with Zhe midrib rice as a male parent and a rice variety of the two-line sterile line as a female parent, hybridizing to obtain F1-generation seeds; selfing the F1-generation seeds; performing back-crossing on fertile rice plants with excellent characters and white midribs, which are selected from an F2-generation plant, and the rice variety of the two-line sterile line to obtain BC1F1-generation seeds; performing back-crossing on an excellent fertile plant which is selected from the BC1F1-generation seeds, and the rice variety of the two-line sterile line to obtain BC2F1-generation seeds; repeating selfing and back-crossing, performing one-time selfing after continuous back-crossing for twice, and performing back-crossing for the last time; and selfing later generations obtained after the last-time back-crossing, selecting sterile single plants with leaves carrying white midribs and excellent characters from the selfed later generations, and developing breeding of a system to obtain the hybrid rice two-line sterile line. In the sterile line obtained by the invention, the marked characters are expressed in the midribs of the leaves only, without influencing the economical characters; hybrids can be simply rejected, and the purity can be identified.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and in particular relates to a method for breeding two-line male sterile lines of hybrid rice with leaves carrying white midribs. Background technique [0002] The annual planting area of ​​hybrid rice in my country accounts for about half of the total planting area of ​​rice, especially the rapid development of two-line hybrid rice. However, it is worth noting that during the seed production of two-line hybrid rice, if there is continuous low temperature and rainy weather, there will often be a phenomenon of fertility conversion, resulting in a decrease in the purity of two-line hybrid rice seeds, which will bring hidden dangers to agricultural production safety. For this reason, breeders have designed to combine leaf color markers with breeding, introduce leaf color markers into sterile lines, and breed sterile lines with leaf color markers. strain. [0003] At present, the most widely ...

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Problems solved by technology

[0004] However, it is worth noting that the existing leaf color markers such as green-turning albino leaf color, purple leaf, yellow leaf, light green and striped vein markers are expressed in large areas in leaves, whi

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