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Method for cultivation of Yellow Sea gadus macrocephalus parent plants and incubation of oosperms

A technology of fertilized eggs and cod, applied in the direction of climate change adaptation, fish farming, application, etc., can solve problems such as inability to ensure sustainable utilization of resources, and achieve the effect of satisfying multiplication and release

Active Publication Date: 2014-10-01
MARINE BIOLOGY INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increasing market demand for aquatic products and continuous high-intensity fishing pressure, relying solely on fish self-replenishment is far from ensuring the sustainable utilization of resources. Carrying out artificial breeding and releasing is an inevitable way to protect and restore damaged fishery resources. Whether the fully artificial large-scale breeding of seedlings can be realized is the basic condition for multiplication and release
There are no detailed reports in China on the artificial cultivation of high-quality broodstock and high-quality embryo cultivation of North Pacific cod

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Parental cultivation

[0016] On January 22, 2013, 54 tails of cod (body length 40-60 cm) were caught by gill nets from the coastal waters of Qingdao, and transported to the Haifu nursery in Donggang District, Rizhao. Parental individuals with healthy physique, no symptoms and obvious trauma were screened out; they were temporarily raised in a 6×6×1.2m cement pool, inflated by an air pump, controlled by a 25W incandescent lamp, and medicated with florfenicol 3ppm, and changed every 2 days Water once, change the water volume 1 / 3. In the temperature range of 2-8°C, experienced a temperature fluctuation: the broodstock was temporarily kept in the indoor cement pond, from December 28, 2013 to January 8, 2014, the water temperature experienced a gradual warming process of 3-6.5°C, 6-6.5°C 8°C was maintained for 4 days; from January 12, 2014 to January 19, 2014, the water temperature experienced a gradual cooling process of 6.0-3°C, and the water temperature has been main...

Embodiment 2

[0023] This embodiment is carried out simultaneously with Example 1, and the number of parents cultivated is also the same. During the cultivation of the parents, except that there is no temperature fluctuation stimulation, all the other cultivation operating conditions are completely the same as in Embodiment 1, and the cultivation water temperature is maintained at 3-4°C. until the parental gonads mature. As of January 26, 2014, there were 105 broodstock remaining, with an average weight of 1.56kg per broodstock, a male-to-female ratio of 1:1, and a total survival rate of 81%. Adopt the same method of embodiment 1 to carry out ovum collection, artificial insemination and hatching, collect 600g of fertilized eggs altogether, hatch 50,000 tails of larvae, hatching rate 13.73%, embodiment 2 is compared with the result of embodiment 1, it can be known that the parents undergo temperature fluctuations Stimulation can improve the degree of sexual maturity and the quality of sperm ...

Embodiment 3

[0025] Incubation temperature gradient experiment

[0026] Set five water temperature gradients of 6°C, 8°C, 10°C, 12°C, and 14°C, and set 2 parallel groups for each gradient. The rest of the environmental conditions are the same as in Example 1. Get 100 fertilized eggs at the multicellular stage, put them in a 1000mL beaker, and inflate them slightly. The beaker is placed in the incubator, and the temperature is controlled by an automatic temperature controller. Record the 50% hatching membrane time, and count the hatching rate. The results showed that fertilized eggs could develop normally to hatch at all temperatures except 14°C when no fertilized eggs hatched, and the rate of normal development to hatch at 6°C and 8°C was the highest. The incubation time of fertilized eggs has an exponential function relationship with the incubation temperature. The higher the temperature, the faster the development, but the hatching rate decreases with the increase of temperature. It s...

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PUM

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Abstract

The invention discloses a method for cultivation of Yellow Sea gadus macrocephalus parent plants and incubation of oosperms, and belongs to the technical field of aquiculture seed cultivation. The method comprises the steps of parent plant cultivation and ovum pick-up, and artificial insemination and incubation; the parent plant cultivation temperature is at 2-8 DEG C; temperature fluctuation stimulation for one time is conducted during the parent plant cultivation period; the temperature stimulation method is that the water temperature uniformly and progressively rises to 6-8 DEG C from 2-3 DEG C; the temperature rising period lasts for 7-9 days; the temperature of 6-8 DEG C lasts for about 4-5 days; then the water temperature progressively and uniformly reduces to 3-4 DEG C from 6-8 DEG C; temperature reduction period lasts for 6-8 days; then the water temperature maintains at 3-4 DEG C; illumination intensity is adjusted to 10001 lx-15001 lx; salinity is at 2.8-3.3%; pH value is at 7.0-8.5; the cultivation is conducted in running water. According to the invention, the temperature stimulation is conducted during the parent plant cultivation process, so as to conducive to sex gland development of the parent plants; incubation is conducted on oosperms adopting the method; the hatchability is about 41.2%.

Description

technical field [0001] The invention belongs to the technical field of aquaculture seed cultivation, and in particular relates to parental cultivation of yellow sea cod and a fertilized egg hatching method thereof. Background technique [0002] Thickhead cod, commonly known as big head fishy, ​​English name Pacific cod, scientific name Gadus macrocephalus, belongs to the cod family, cod species, is a cold water species widely distributed along the North Pacific coast, from the North Yellow Sea in my country along the edge of the North Pacific shelf to the United States Santa Monica Bay, California. In my country, the reason why there are cold-water fishes in the Yellow Sea is closely related to the existence of cold water masses in the Yellow Sea. Thickhead is one of the most important catch species in the North Pacific Ocean because of its large resources. In recent years, the world's output has been between 400,000 and 500,000 tons, and the highest in my country's history...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 于道德姜云荣刘洪军官曙光刘名
Owner MARINE BIOLOGY INST OF SHANDONG PROVINCE
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