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A method for efficiently extracting polysaccharides produced by submerged liquid-state fermentation of P.

A technology of Anluo small skin umbrella and mycelium polysaccharide, applied in the field of microorganisms, can solve the problems of increased pollution control cost, high labor intensity, long operation cycle, etc., and achieves reduction of pollution control cost, labor intensity, and labor intensity. Effect

Active Publication Date: 2017-01-04
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this process is that a large amount of water needs to be added for a long time to extract, and in order to increase the yield, it needs to be extracted repeatedly for 2-3 times. The operation cycle is long and the labor intensity is too high. The extraction yield of polysaccharides is mostly 2-3%. , the product cost is more expensive
And the separated supernatant is directly discarded as waste liquid, which increases the cost of pollution control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The present invention efficiently extracts the method for producing the mycelia polysaccharide of Anluo phellodermis by deep liquid fermentation according to the following steps:

[0028] 1. Preparation of Anluo Xiaopijiao fermentation liquid: Anluo Xiaopijiao strain was purchased from the General Microbiology Center of the Institute of Microbiology, Chinese Academy of Sciences, and the strain code is CGMCC NO5.0152. Inoculate the activated Anluo small umbrella shake flask bacterial strain into the seed medium (10L) according to the inoculum size 10% (v / v), wherein the seed medium consists of 3% glucose, 0.3% yeast powder, and 1.0% corn steep liquor , Potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 6.5, the ventilation ratio is 1:1.0vvm, the tank pressure is 0.03Mpa, shake culture at 24-26°C for 5 days to get it The seed liquid of Umbriatus spp.; the seed liquid is inserted into the fermentation medium (5...

Embodiment 2

[0036] The present invention efficiently extracts the method for producing the mycelia polysaccharide of Anluo phellodermis by deep liquid fermentation according to the following steps:

[0037] 1. Preparation of Anluo Xiaopijiao fermentation liquid: Anluo Xiaopijiao strain was purchased from the General Microbiology Center of the Institute of Microbiology, Chinese Academy of Sciences, and the strain code is CGMCC NO5.0152. Inoculate the activated Anluo Xiaopisha shake flask strain into the seed medium (20L) according to the inoculum size 15% (v / v), wherein the seed medium consists of 3% glucose, 0.3% yeast powder, and 1.0% corn steep liquor , Potassium dihydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, adjust the pH value to 6.5, the ventilation ratio is 1:1.0vvm, the tank pressure is 0.03Mpa, shake culture at 26-28°C for 5 days to get it The seed liquid of Umbriatus spp.; the seed liquid is inserted into the fermentation medium (100L) according to...

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PUM

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Abstract

The invention discloses a method for efficiently extracting deep-layer liquid fermentation to produce the mycelia polysaccharides of Anluo Xiaopijiao mycelia. Get Anluo Xiaopi polysaccharide. The invention changes the traditional polysaccharide extraction method of "water extraction and alcohol precipitation", improves the extraction yield and product quality of the polysaccharide of Anluo Xiaopi polysaccharide, reduces the consumption of purified water and energy consumption in production, and increases economic benefits.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for efficiently extracting deep-layer liquid-state fermentation to produce polysaccharides from Dermodium parvulari mycelia. Background technique [0002] Anluo small umbrella, the scientific name is Marasmius androsaceus (L.ex Fr.) Fr. Alias ​​ghost hair needle, tea brown small umbrella, etc., belongs to Basidiomycetes, Agaricales (Agaricales), White Mushroom (ThichoLomataceae) , Small umbrella (Marasmius). Born in deep mountains and dense forests, cold and humid, acidic soil above dead branches, mainly distributed in Jilin, Hunan, Hubei, Guangdong and other provinces of China. The folks in our country have long used the rhizome cord of the wild Anluo Xiaopi umbrella to treat fracture pain, bruises, etc. According to the report of Guangdong Institute of Microbiology (1975), the Institute and Guangzhou No. 1 Pharmaceutical Factory obtained the fermentation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/04
Inventor 王文风徐国华徐玲王英燕张芙蓉杨亚威高岩
Owner JIANGSU SHENHUA PHARMA
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