Preparation of three-dimensional photoelectrochemical paper chip and application of three-dimensional photoelectrochemical paper chip in tumor detection

A photoelectrochemical and paper chip technology, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve the problems of poor conductivity of paper chips, no signal amplification, low content detection of tumor markers, etc.

Inactive Publication Date: 2014-10-29
UNIV OF JINAN
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Problems solved by technology

However, the electrical conductivity of a single paper chip is very poor, and it does not have the effect of signal amplification. If the paper fiber is connected with gold, silver, platinum and other noble metal nanoparticles with large specific Nanomaterials such as zinc oxide, cadmium sulfide quantum dots, etc., then the functionalized high-throughput microfluidic paper chip analysis device we obtained can achieve signal amplification well, and then realize low-level detection of tumor markers

Method used

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  • Preparation of three-dimensional photoelectrochemical paper chip and application of three-dimensional photoelectrochemical paper chip in tumor detection
  • Preparation of three-dimensional photoelectrochemical paper chip and application of three-dimensional photoelectrochemical paper chip in tumor detection
  • Preparation of three-dimensional photoelectrochemical paper chip and application of three-dimensional photoelectrochemical paper chip in tumor detection

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Embodiment example 1

[0026] Preparation of a photoelectrochemical three-dimensional paper chip and its application in tumor detection

[0027] (1) Use Adobe illustrator CS4 to design photoelectrochemical three-dimensional microfluidic paper chip hydrophobic wax batch printing patterns on the computer;

[0028] (2) Cut the paper into a commonly used A4 size paper, print the wax hydrophobic pattern on the paper using wax printing technology, and keep the printed wax pattern at a constant temperature of 150 oC for 2 minutes to melt the wax and soak the entire thickness of the paper to form a hydrophobic pattern. The wall, the part without printing wax is the hydrophilic area, which is the working area;

[0029] (3) Electrode printing is performed by screen printing technology. Using graphite as raw material, take an equal volume of acetone and cyclohexanone solution, then add the corresponding amount of cellulose acetate, use ultrasonic ultrasound to fully dissolve the cellulose acetate in the mixed...

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Abstract

The invention discloses a preparation method for a three-dimensional photoelectrochemical paper chip and a method for measuring six tumor markers in a sample through a photoelectrochemical sensor. A hydrophobic region and a hydrophilic region are formed on paper by adopting a wax printing technology; a corresponding reference electrode and working electrodes are printed on the paper through proper ink; a working region is functionalized for antigen recognition; the prepared paper chip is folded to form a three-electrode system; a buffering solution containing ascorbic acid is dropwise added into a reaction region; under the irradiation of an ultraviolet lamp, the high-sensitivity detection on an object to be measured is realized.

Description

technical field [0001] The invention relates to the technical field of photoelectrochemical analysis and detection, and more specifically relates to the construction of a microfluidic paper-on-a-chip laboratory technology platform suitable for photoelectrochemical analysis. Background technique [0002] In recent years, the national cancer morbidity and mortality have shown a trend of increasing gradually. Therefore, it is very important to find a rapid, quantitative and sensitive method for detecting tumor markers in early detection and treatment monitoring. There are two common methods of clinical diagnosis: detection through relatively expensive large-scale instrumental analysis equipment in hospitals; and rapid and sensitive on-site analysis and diagnosis through small and simple handheld devices. However, in clinical testing, the detection of a single tumor marker often leads to false positive results, while the joint detection of multiple tumor markers can improve the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N27/26
CPCG01N33/54386G01N33/574
Inventor 于京华李丽颜梅葛慎光张彦吴鲁丹孙国强吴开庆
Owner UNIV OF JINAN
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