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Culture medium for dendrobium tissue cultivation

A technology of tissue culture and culture medium, applied in the field of culture medium for dendrobium tissue culture, which can solve the problems of increasing the price of dendrobium products, poor culture effect, and unavailability, and achieves low cost, simple and easy-to-obtain components, and increased production and quality effects

Inactive Publication Date: 2014-12-17
江苏神草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of tissue culture of Dendrobium candidum, culture medium is needed, but the existing culture medium for Dendrobium candidum is not effective and expensive, which increases the price of Dendrobium products, and can not obtain the Dendrobium with good medicinal effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A culture medium for dendrobium tissue culture is provided, which can be used as a proliferation medium. The medium is based on MS medium, and the added components include: α-naphthaleneacetic acid (NAA) 0.1g / L, 6- Benzyl adenine (6-BA) 0.2g / L, proline 0.3g / L, sugar 35g / L, banana 100g / L, agar 3.7g / L.

Embodiment 2

[0016] Provide a medium for dendrobium tissue culture, which can be used as a rooting medium. The components of the medium are based on 1 / 2 MS medium, and the added components include: α-naphthaleneacetic acid (NAA) 0.2 g / L, 6-benzyl adenine (6-BA) 0.1g / L, proline 0.3g / L, sugar 35g / L, banana 100g / L, agar 3.7g / L.

[0017] The beneficial effects of the present invention are: the culture medium for dendrobium tissue culture of the present invention, take MS culture medium as basic medium, add the remaining components, the components used are simple and easy to get, the cost is low, the dendrobium grows well, greatly improves the yield and quality, can be used in various fields in the research, development and production of Dendrobium, and is especially suitable for large-scale industrial production.

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PUM

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Abstract

The invention discloses a culture medium for dendrobium tissue cultivation. MS (Murashige and Skoog) culture medium is served as a basal culture medium and added components comprise 1-naphthalene acetic acid, 6-benzylaminopurine, proline, sugar, bananas and agar. According to the culture medium, the MS culture medium is served as the basal culture medium, other components are added, utilized components are easy to obtain, costs are low, the dendrobium grows well, the yield and the quality are greatly improved, and the culture medium can be applied to dendrobium research, development and fields in production and is particularly applicable to large scale industrialized production.

Description

technical field [0001] The invention relates to the field of dendrobium cultivation, in particular to a culture medium for dendrobium tissue culture. Background technique [0002] Dendrobium candidum is a perennial herb belonging to epiphytic orchids. It is commonly known as Tiepi Fengdou. Because of its black outer stems, it is also known as black knot grass. Dendrobium is a commonly used precious traditional Chinese medicine with a long history of application. In "Shen Nong's Herbal Classic" Was listed as top grade. It has been proven that Dendrobium has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immune function, preventing tumor and cardiovascular disease, improving sleep, preventing cataract, and prolonging life. However, due to the special growth environment, limited distribution, and long-term mining, the natural resources...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冯小龙
Owner 江苏神草生物科技有限公司
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