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A kind of potassium decomposing bacterium and application thereof

A potassium-solubilizing bacteria and potassium-solubilizing technology, applied in the field of microorganisms, can solve problems such as affecting agricultural development, decreasing organic matter content, destroying soil structure, etc., achieving good economic and social effects, promoting growth and development, and simple production processes.

Active Publication Date: 2017-02-15
轩凯生物科技(山东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] About 60% of the arable land in my country is deficient in potassium, and the content of available potassium in arable land is increasing at an annual rate of (2-3)×10 -6 The rate of decline of the soil causes the imbalance of nitrogen, phosphorus and potassium in the soil, which affects the development of agriculture.
Supplementing potassium with chemical potassium fertilizer is a quick-acting potassium supplement method commonly used in my country's agriculture, but it causes soil structure damage, organic matter content decline, serious pollution, high cost, and short supply.

Method used

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  • A kind of potassium decomposing bacterium and application thereof
  • A kind of potassium decomposing bacterium and application thereof
  • A kind of potassium decomposing bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of bacterial agent of potassium-dissolving bacteria provided by the invention, it is prepared by the following method:

[0041] Bacillus subtilis JT-2 stored on a slant at 4°C was activated, inoculated on eggplant bottle medium (ie activation medium), and cultured for 24 hours. Pick a large number of strains from the eggplant bottle, inoculate them in the fermentation medium, and cultivate them at 30°C for 16 hours to obtain the fermentation liquid or the dry product of the fermentation liquid. The number of bacteria in the bacterial agent can reach 1×10 10 -9×10 12 CFU / g.

[0042] The medium in the described eggplant-shaped culture bottle is LB medium, and the fermentation medium component is: glucose 8-12g / L, (NH 4 ) 2 SO 4 0.05-1g / L, NaCl 0.1-1g / L, MnSO 4 4H 2 O 0.1-0.5g / L, KH 2 PO 4 0.5-2.0g / L, pH 6.0-8.0.

[0043] During the fermentation and cultivation process of the above-mentioned production tank, the ventilation rate of sterile air is 0.6-0.8v...

Embodiment 2

[0044] Example 2: Determination of the potassium-dissolving ability of the strain.

[0045] Prepare potassium-solubilizing medium (without water-soluble potassium ions) in 95 mL subpackages, add 0.5 g of accurately weighed potassium ore powder into a 250 mL Erlenmeyer flask, sterilize at 121 ° C for 30 min, and inoculate the bacterial suspension to be tested with 5% inoculum Into the shake flask strains, each strain was done in triplicate, and no inoculation control was set at the same time. After 25 days of culture in a water bath shaker at 37°C, the culture was terminated. The culture solution was centrifuged at 1000 r / min at 4°C for 5 minutes, and 4 mL of the centrifuged supernatant and 4 mL of 6 mmoL / LiCl were thoroughly mixed with a pipette, and potassium was measured by a flame spectrophotometer. It is obtained that the water-soluble potassium ion content is 118-132mg / L, and the potassium-dissolving rate reaches 45.4%-50.8%, which has a strong potassium-dissolving abilit...

Embodiment 3

[0046] Example 3: Potassium-dissolving bacterium can improve crop yield.

[0047] The fermentation preparation of potassium decomposing bacteria agent is basically the same as embodiment 1.

[0048]Adopt pot method, take wheat as experimental object, inoculate with the potassium-dissolving bacterial agent cultivated and prepared by the present invention, test establishes 2 treatments: blank control and apply bacterial agent, applied bacterial agent is every kilogram of soil 8mg. Detect various indicators such as wheat yield at the maturity stage of wheat.

[0049] Table 1. The growth status of wheat under different treatment conditions in the wheat pot experiment

[0050]

[0051] In the wheat pot experiment, the potassium-dissolving bacterium obviously promoted the growth of wheat, and the yield increased by 36.4%.

[0052] The variety of melon to be tested: xuelihong, and the test set up 2 treatments: blank control and application of bacterial agents.

[0053] Table 2....

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Abstract

The invention discloses an efficient potassium bacterium. The efficient potassium bacterium is classified to be named as Bacillus subtilis, has the strain number of JT-2, and is collected in the China Center for Type Culture Collection (CCTCC); and the strain collection number is CCTCC M2014368, and the collection date is August 5, 2014. The invention also discloses an application of the efficient potassium bacterium in soil potassium decomposition. By adopting the efficient potassium bacterium, insoluble silicon aluminate inorganic minerals such as potash feldspar and apatite can be effectively decomposed, difficultly-soluble potassium elements can be promoted to be transformed into soluble nutrients, the content of soil available nutrients can be increased, the use amount of a chemical fertilizer can be reduced, the growth and development of crops can be promoted, and the yield can be increased. The efficient potassium bacterium disclosed by the invention has important significance and application values in aspects of cultivating and playing the soil ecological fertility and keeping the agricultural ecological balance.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a potassium-dissolving bacterium and its application. Background technique [0002] Potassium is one of the three elements of crop nutrition. It is ubiquitous in crops. Its function is related to plant metabolism. It widely affects the growth and metabolism of crops in the form of an enzyme activator, which can activate enzymes. It has been found that about The activation of more than 60 enzymes is related to potassium, which plays a very important role in physiological processes such as light energy utilization, sugar metabolism, protein synthesis, cell osmotic regulation and enhancement of plant resistance. [0003] About 60% of the arable land in my country is deficient in potassium, and the content of available potassium in arable land is increasing at an annual rate of (2-3)×10 -6 The rate of decline caused the imbalance of nitrogen, phosphorus and potass...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/125
Inventor 冯小海徐虹许宗奇梁金丰李莎
Owner 轩凯生物科技(山东)有限公司
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