A kind of potassium decomposing bacterium and application thereof
A potassium-solubilizing bacteria and potassium-solubilizing technology, applied in the field of microorganisms, can solve problems such as affecting agricultural development, decreasing organic matter content, destroying soil structure, etc., achieving good economic and social effects, promoting growth and development, and simple production processes.
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Embodiment 1
[0040] A kind of bacterial agent of potassium-dissolving bacteria provided by the invention, it is prepared by the following method:
[0041] Bacillus subtilis JT-2 stored on a slant at 4°C was activated, inoculated on eggplant bottle medium (ie activation medium), and cultured for 24 hours. Pick a large number of strains from the eggplant bottle, inoculate them in the fermentation medium, and cultivate them at 30°C for 16 hours to obtain the fermentation liquid or the dry product of the fermentation liquid. The number of bacteria in the bacterial agent can reach 1×10 10 -9×10 12 CFU / g.
[0042] The medium in the described eggplant-shaped culture bottle is LB medium, and the fermentation medium component is: glucose 8-12g / L, (NH 4 ) 2 SO 4 0.05-1g / L, NaCl 0.1-1g / L, MnSO 4 4H 2 O 0.1-0.5g / L, KH 2 PO 4 0.5-2.0g / L, pH 6.0-8.0.
[0043] During the fermentation and cultivation process of the above-mentioned production tank, the ventilation rate of sterile air is 0.6-0.8v...
Embodiment 2
[0044] Example 2: Determination of the potassium-dissolving ability of the strain.
[0045] Prepare potassium-solubilizing medium (without water-soluble potassium ions) in 95 mL subpackages, add 0.5 g of accurately weighed potassium ore powder into a 250 mL Erlenmeyer flask, sterilize at 121 ° C for 30 min, and inoculate the bacterial suspension to be tested with 5% inoculum Into the shake flask strains, each strain was done in triplicate, and no inoculation control was set at the same time. After 25 days of culture in a water bath shaker at 37°C, the culture was terminated. The culture solution was centrifuged at 1000 r / min at 4°C for 5 minutes, and 4 mL of the centrifuged supernatant and 4 mL of 6 mmoL / LiCl were thoroughly mixed with a pipette, and potassium was measured by a flame spectrophotometer. It is obtained that the water-soluble potassium ion content is 118-132mg / L, and the potassium-dissolving rate reaches 45.4%-50.8%, which has a strong potassium-dissolving abilit...
Embodiment 3
[0046] Example 3: Potassium-dissolving bacterium can improve crop yield.
[0047] The fermentation preparation of potassium decomposing bacteria agent is basically the same as embodiment 1.
[0048]Adopt pot method, take wheat as experimental object, inoculate with the potassium-dissolving bacterial agent cultivated and prepared by the present invention, test establishes 2 treatments: blank control and apply bacterial agent, applied bacterial agent is every kilogram of soil 8mg. Detect various indicators such as wheat yield at the maturity stage of wheat.
[0049] Table 1. The growth status of wheat under different treatment conditions in the wheat pot experiment
[0050]
[0051] In the wheat pot experiment, the potassium-dissolving bacterium obviously promoted the growth of wheat, and the yield increased by 36.4%.
[0052] The variety of melon to be tested: xuelihong, and the test set up 2 treatments: blank control and application of bacterial agents.
[0053] Table 2....
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