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Ensifer adhesion and application thereof in preparation of microbial fertilizer

A technology of microorganisms and microbial strains, applied in microorganism-based methods, microorganisms, biochemical equipment and methods, etc., to achieve the effect of promoting growth and development, improving its own metabolic system, and maintaining balance

Active Publication Date: 2022-07-01
苏州科宁多元醇有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: In order to solve the problem of plant resistance to salt, the present invention provides a bacterium adhesica, and the present invention further provides an inoculum in which the inoculum is an active microorganism and the effect of the inoculum in enhancing the salt stress resistance of crops. application

Method used

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  • Ensifer adhesion and application thereof in preparation of microbial fertilizer
  • Ensifer adhesion and application thereof in preparation of microbial fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening and identification of S. adherens

[0026] Adhesion to the sword fungus CN-02 is a bacterial strain selected by the inventor from the paddy soil of Tangquan Town, Pukou District, Nanjing City (with the production of polysaccharide as the standard). The screening process is as follows:

[0027] Dissolve the collected soil samples in sterile water, shake and mix well. The sample solution was serially diluted, taking 10 -4 , 10 -5 , 10 -6 The dilutions of three concentration gradients were evenly spread on solid medium (medium components: sucrose 20 g / L, peptone 5 g / L, beef extract 1 g / L, NaCl 5 g / L, agar 20 g / L). Placed in a 30°C constant temperature incubator for 48 hours, during which the plates were checked every day to observe the characteristics of the colonies, and the sticky colonies were used as the screening target. A single colony capable of producing high-viscosity exopolysaccharide was picked and purified by streaking method on a solid ...

Embodiment 2

[0031] Embodiment 2 Staphylococcus aureus CN-02 microbial inoculum

[0032]The E. adhaerens CN-02 stored on a slant at 4°C was activated and inoculated on the eggplant bottle medium (ie, the activation medium), the activation temperature was 30°C, and the activation time was 16h. Pick a large amount of bacteria on the eggplant bottle and inoculate it into the fermentation medium. The ventilation volume of the sterile air in the fermentation tank is 0.2vvm, the stirring speed is 200rpm, and the fermentation liquid is obtained by culturing at 30 °C for 48h. The number of bacteria in the bacteria agent up to 5×10 9 CFU / g or more.

[0033] The activation medium is LB medium, and the components of the fermentation medium are: glucose 40 g / L, beef extract 8 g / L, NaCl 6 g / L, peptone 10 g / L, and pH 7.0.

Embodiment 3

[0034] Embodiment 3 Determination of the ability of S. glutinosa CN-02 to produce extracellular polysaccharides

[0035] The exopolysaccharide content in the fermentation broth of Example 2 was quantitatively detected by the phenol-sulfuric acid method, and the amount of exopolysaccharide produced by S. glutinosa CN-02 was calculated according to the standard curve made by anhydrous glucose. The results showed that the production of exopolysaccharide of S. glutinosa CN-02 reached 7.4 g / L.

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Abstract

The invention discloses an ensifer adhesion strain, which is classified and named as ensifer adhesion, has a strain number of CN-02, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC NO. 21357. The ensifer adhesion strain has the advantages that the ensifer adhesion strain is named as ensifer adhesion, the strain number is CN-02, and the ensifer adhesion strain is named as ensifer adhesion strain; the invention further discloses application of the ensifer adhesion in enhancing the salt stress resistance of crops. The invention discloses a microbial agent taking ensifer adhesion as an active microorganism. The invention further discloses application of the microbial inoculum in enhancing salt stress resistance of crops. The ensifer adhesion can be adhered to the root system of the plant, can also enter the plant to be symbiotic with the plant, improves the absorption of the plant to external nutrient elements, improves the metabolism system of the plant, maintains the internal water potential of the plant and the like, thereby promoting the growth and development of the plant, increasing the yield and enhancing the salt stress resistance of the plant. And the method has extremely important significance and application value for fully exerting the ecological fertility of the soil, keeping the balance of the agricultural ecological environment and the like.

Description

technical field [0001] The present invention relates to the field of microbial inoculants, in particular to a kind of Vibranium Adhesalis and its inoculum and application. Background technique [0002] Soil salinization and secondary soil salinization caused by irrigation are a major environmental risk caused by natural or human activities, usually appearing in arid climate, high soil evaporation intensity, high groundwater table and high content of soluble salts. area. According to statistics, 20% of the world's arable land and nearly half of the irrigated land are threatened by different degrees of salinity, and the proportion is still rising. The total area of ​​saline land in my country is about 100 million hectares, mainly distributed in 17 provinces including northwest, northeast, north China and coastal areas. When the soil salt concentration reaches a certain value, it will destroy the normal life activities of plant cells and inhibit the growth of plants, resultin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12N1/20C12P19/04Y02A40/10
Inventor 沙凤孙科
Owner 苏州科宁多元醇有限公司
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