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A kind of penicillium aspenicillium and its bacterial agent and application

A technology of Penicillium aspenicillium and strains, applied in the direction of application, fungicide, biocide, etc., can solve problems such as salinization, plant diseases, calcareous soil, etc., achieve improved resistance, simple production process, and high production efficiency Effect

Active Publication Date: 2019-12-03
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by this invention is to provide a kind of Penicillium aspenicillium to solve problems such as plant disease, salinization and calcareous soil

Method used

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  • A kind of penicillium aspenicillium and its bacterial agent and application
  • A kind of penicillium aspenicillium and its bacterial agent and application
  • A kind of penicillium aspenicillium and its bacterial agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Screening and identification of P. asturianum XK-12

[0043] P.asturianumXK-12 is a bacterial strain selected and bred by the inventor from strawberry rhizosphere soil (based on the antagonistic effect of Fusarium oxysporum and resistance to salt stress). The screening process is as follows:

[0044] Dissolve the collected soil samples in sterile water, shake and mix well. The sample solution was serially diluted, taking 10 -4 、10 -5 、10 -6 The dilutions of three concentration gradients were evenly spread on the PDA medium (medium composition: potato 200g, glucose 20g, agar 20g, sodium chloride 100g distilled water 1000mL) containing 20% ​​NaCl. Place it in a constant temperature incubator at 28°C for 3-5 days, transfer a small number of colonies grown to the edge of a blank PDA plate, place Fusarium oxysporum as an indicator bacterium in the middle, observe the characteristics of the colonies, and use the antagonistic effect as the screening target. A si...

Embodiment 2

[0050] Embodiment 2: P.asturianumXK-12 bacterial strain biological characteristics

[0051] The P. asturianumXK-12 stored on the slant at 4°C was activated and inoculated on PDA medium (ie, the activation medium). The activation temperature was 28°C and the activation time was 2 days. Use a 0.8cm hole punch to transfer fresh bacteria cakes to empty inorganic phosphorus medium A, organic phosphorus medium B, cellulose Congo red medium C, pH8-11 PDA medium (D-G) and 5%-20% NaCl The PDA medium (H-J) was cultured at 28°C for 5 days to observe the colony morphology.

[0052] Inorganic phosphorus medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g,

[0053] MgSO 4 ·7H 2 O 0.3g, CO 3 (PO 4 ) 2 10g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O0.03g, distilled water 1000mL, pH 7.0~7.5;

[0054] Organic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, yeast extract powder 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate 0.3g, ferrous sulfat...

Embodiment 3

[0059] Example 3: Plate confrontation experiment of P.asturianumXK-12 against fungal disease pathogens

[0060] P. asturianum XK-12 and 6 kinds of pathogenic fungi (Colletotrichum gloeosporioide, Neofusicoccum kwambonambiense, Fusarium oxysporun, Pythium helicoides, Ceratobasidium sp.AG-A and Fusarium solani) were inoculated on PDA at the same time, cultured at 28°C for three days, and the control group have grown over the entire plate, and the antagonistic effect is as follows Figure 4 As shown, the inhibitory rate to Neofusicoccum kwambonambiense reaches 95%, and the antagonistic effect to Ceratobasidium sp.AG-A reaches 45%. The experiment proves that the strain has a broad-spectrum disease-resistant effect.

[0061] Mycelia growth inhibition rate (%)=(blank control colony growth diameter-treatment colony growth diameter) / blank control colony growth diameter×100

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Abstract

The invention discloses a Penicillium asturianum, which is classified as Penicillium asturianum (Penicillium asturianum), and the strain number is XK-12, which has been preserved in the China Common Microorganisms Collection and Management Center, and the preservation time is March 2019 On the 28th, the deposit number is CGMCC No.17198. The invention also discloses the application of the above-mentioned Penicillium aspenicillium. The bacterial strain of the present invention has excellent properties such as high temperature resistance, salt and alkali resistance, cellulase production, promotion of iron absorption and phosphorus dissolution, and can be applied to barren, saline-alkali land and calcareous soil restoration, straw decomposing and compost bacterial agent inoculation and the like. The P. asturianum XK-12 bacterial strain of the invention has a large amount of spore production, is highly efficient, has a broad-spectrum plant disease biocontrol ability, can promote plant growth, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of microbial bacterial agents, in particular to a P. asturianum, bacterial agents and applications thereof. Background technique [0002] P. asturianum XK-12 belongs to the phylum Ascomycota, the class Eurotiomycetes, the order Eurotiales, and the family Trichocomaceae. The strain has a biosafety risk level of 1, and has excellent characteristics such as saline-alkali tolerance, high phosphorus solubility, high-yield cellulase decomposing straw, and significant antagonistic effect on broad-spectrum plant diseases. As an organic fertilizer returning to the field in situ, microbial antibacterial agents and their metabolites have broad application prospects in biopesticides and other aspects. The strain has a high spore production rate, can produce spores while growing, has a high level of solid-state fermentation and liquid fermentation, and is simple in process, suitable for industrial production. Balance and so on h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G3/00C05G3/04C05F11/08A01N63/04A01N37/46A01P3/00A01P21/00C09K17/14C12R1/80C09K101/00C05G3/80
CPCC05G3/00C05F11/08A01N37/46C09K17/14C09K2101/00C05G3/80C12R2001/80C12N1/145A01N63/30
Inventor 徐虹詹伊婧彭雯杰冯小海许宗奇王明轩
Owner NANJING TECH UNIV
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