A kind of penicillium aspenicillium and its bacterial agent and application
A technology of Penicillium aspenicillium and strains, applied in the direction of application, fungicide, biocide, etc., can solve problems such as salinization, plant diseases, calcareous soil, etc., achieve improved resistance, simple production process, and high production efficiency Effect
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Embodiment 1
[0042] Example 1: Screening and identification of P. asturianum XK-12
[0043] P.asturianumXK-12 is a bacterial strain selected and bred by the inventor from strawberry rhizosphere soil (based on the antagonistic effect of Fusarium oxysporum and resistance to salt stress). The screening process is as follows:
[0044] Dissolve the collected soil samples in sterile water, shake and mix well. The sample solution was serially diluted, taking 10 -4 、10 -5 、10 -6 The dilutions of three concentration gradients were evenly spread on the PDA medium (medium composition: potato 200g, glucose 20g, agar 20g, sodium chloride 100g distilled water 1000mL) containing 20% NaCl. Place it in a constant temperature incubator at 28°C for 3-5 days, transfer a small number of colonies grown to the edge of a blank PDA plate, place Fusarium oxysporum as an indicator bacterium in the middle, observe the characteristics of the colonies, and use the antagonistic effect as the screening target. A si...
Embodiment 2
[0050] Embodiment 2: P.asturianumXK-12 bacterial strain biological characteristics
[0051] The P. asturianumXK-12 stored on the slant at 4°C was activated and inoculated on PDA medium (ie, the activation medium). The activation temperature was 28°C and the activation time was 2 days. Use a 0.8cm hole punch to transfer fresh bacteria cakes to empty inorganic phosphorus medium A, organic phosphorus medium B, cellulose Congo red medium C, pH8-11 PDA medium (D-G) and 5%-20% NaCl The PDA medium (H-J) was cultured at 28°C for 5 days to observe the colony morphology.
[0052] Inorganic phosphorus medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g,
[0053] MgSO 4 ·7H 2 O 0.3g, CO 3 (PO 4 ) 2 10g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O0.03g, distilled water 1000mL, pH 7.0~7.5;
[0054] Organic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, yeast extract powder 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate 0.3g, ferrous sulfat...
Embodiment 3
[0059] Example 3: Plate confrontation experiment of P.asturianumXK-12 against fungal disease pathogens
[0060] P. asturianum XK-12 and 6 kinds of pathogenic fungi (Colletotrichum gloeosporioide, Neofusicoccum kwambonambiense, Fusarium oxysporun, Pythium helicoides, Ceratobasidium sp.AG-A and Fusarium solani) were inoculated on PDA at the same time, cultured at 28°C for three days, and the control group have grown over the entire plate, and the antagonistic effect is as follows Figure 4 As shown, the inhibitory rate to Neofusicoccum kwambonambiense reaches 95%, and the antagonistic effect to Ceratobasidium sp.AG-A reaches 45%. The experiment proves that the strain has a broad-spectrum disease-resistant effect.
[0061] Mycelia growth inhibition rate (%)=(blank control colony growth diameter-treatment colony growth diameter) / blank control colony growth diameter×100
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