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Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof

A technology of rhizosphere phosphate solubilizing bacteria and phosphate solubilizing bacteria, applied in application, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problems of microbial ecosystems that have not been reported yet, and achieve the goal of improving biological Effectiveness, improvement of soil structure, effect of promoting root growth

Inactive Publication Date: 2013-06-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus-dissolving bacteria discovered so far are mainly concentrated in the soil of crops, and phosphorus-dissolving microbial fertilizers are also mostly used in the production practice of crops, but there are no relevant reports on the microbial ecosystems cultivated by Chinese medicinal materials such as Angelica sinensis

Method used

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  • Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof
  • Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof
  • Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of Angelica rhizosphere high-efficiency phosphate solubilizer bacterial agent, it is prepared by following method:

[0026] Take Angelica rhizosphere phosphorus-solubilizing bacteria Bacillus sp.DGP01 (preservation number is CGMCC No.4755) bacterial strain and inoculate in the seed culture medium shake flask, 30 ℃, 200 rev / min seed vibration culture to logarithmic growth phase; Then above-mentioned culture A good strain is inoculated into a 500-liter seed tank equipped with a fermentation medium according to a 10% inoculum amount, and the feeding amount is 400 liters, and it is cultivated to the logarithmic growth phase; There are 5 tons of production tanks with fermentation medium for cultivation, and the feeding amount is 4 tons. The culture liquid obtained from fermentation is directly packed into liquid dosage forms of phosphobacteria inoculum in plastic packaging barrels or packaging bottles.

[0027] Above-mentioned seed culture medium, seed tank and the us...

Embodiment 2

[0030] A kind of Angelica rhizosphere high-efficiency phosphate solubilizer bacterial agent, it is prepared by following method:

[0031] Take Angelica rhizosphere phosphorus-solubilizing bacteria Bacillus sp.DGP01 (preservation number is CGMCC No.4755) bacterial strain and inoculate in the seed culture medium shake flask, 30 ℃, 200 rev / min seed vibration culture to logarithmic growth phase; Then above-mentioned culture A good bacterial classification is inoculated into a 500 liter seed tank equipped with a fermentation medium according to a 10% inoculum amount, and the feeding amount is 450 liters, and it is cultivated to the logarithmic growth phase; There are 5 tons of production tanks with fermentation medium for cultivation, and the feeding amount is 4.5 tons. The culture liquid obtained from fermentation is absorbed by peat and packed into solid dosage forms of phosphate-solubilizing bacteria bacterial agents with packaging bags.

[0032] Above-mentioned seed culture med...

Embodiment 3

[0034] Example 3 Phosphorus Solubilizing Ability Test of Phosphorus Solubilizing Bacteria

[0035] 1. Determination of the phosphorus-solubilizing ability of the strain by liquid culture method

[0036] 30 mL of sterilized seed culture medium (glucose 10 g, (NH 4 ) 2 SO 4 0.5g, KCl0.3g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, KH 2 PO 4 1.0g, distilled water 1000mL, pH 7.0~7.5), insert the activated slant Bacillus sp.DGP01 strain (preservation number is CGMCC No.4755), and shake the flask to culture to the logarithmic growth phase (about 10 8 CFU mL -1 ). Insert phosphorus-dissolving culture solution (250mL triangular bottle with 40mL culture medium) according to 5% inoculum amount respectively, set up the control group that does not inoculate strain at the same time, each treatment repeats 5 times, shake at 32 ℃ after inoculation, 200 rev / min constant temperature After culturing on the bed for 7-10 days, take 10ml of the culture ...

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Abstract

The invention discloses an angelica root rhizosphere efficient phosphate-solubilizing bacterium and a microbial inoculum prepared from the same and application thereof. The phosphate-solubilizing bacterium is bacillus sp.DGP01 which is collected in the China General Microbiological Culture Collection Center (Address: Datun Road, Chaoyang District, Beijing, 100101) with the collection number CGMCCNo.4755 on April 7, 2011. The microbial inoculum of the phosphate-solubilizing bacterium is prepared by the following steps of: inoculating a bacillus sp.DGP01 strain into a seed culture medium shakeflask and performing shaking culturing for an exponential growth phase; inoculating the cultured strain into a seeding tank which is filled with a fermentation culture medium and culturing for an exponential growth phase; and inoculating the fermentation liquor into a production tank which is filled with a fermentation culture medium for culturing. By adopting the phosphate-solubilizing bacterium, slightly-soluble inorganic phosphorus can be transformed into high-quality phosphorus compounds which can be directly absorbed and utilized by plants, the biological effectiveness of slightly-soluble phosphorus in soil can be enhanced, the utilization efficiency of a phosphorus fertilizer is increased, the fertilizer is saved, and the yield is increased; and moreover, the phosphate-solubilizing bacterium plays an important role in culturing and utilizing the ecological fertility of soil and keeping the ecological balance of agriculture, and has an application value.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to Bacillus sp.DG01, a high-efficiency phosphate-solubilizing bacterium in the rhizosphere of Angelica sinensis, and the bacterial preparation prepared by fermentation and its application. Background technique [0002] Phosphorus is one of the mineral elements necessary for plant growth and development. It is an important component of nucleic acids, enzymes, coenzymes, and ATP in plants. The amount of phosphorus absorbed by crops is significantly positively correlated with its biomass and yield. When plants are deficient in phosphorus, There will be reduced or delayed tillering, delayed heading, late maturity, reduced number of grains per ear, unfulfilled grains, bald corn ears, rape pods, sweet potatoes, and smaller potato pieces. Therefore, phosphorus is extremely important for agricultural production. However, 74% of the arable land in my country is deficient in p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C09K17/00C12R1/07C09K101/00
Inventor 江曙段金廒钱大玮陶金华
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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