Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof
A technology of rhizosphere phosphate solubilizing bacteria and phosphate solubilizing bacteria, applied in application, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problems of microbial ecosystems that have not been reported yet, and achieve the goal of improving biological Effectiveness, improvement of soil structure, effect of promoting root growth
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Embodiment 1
[0025] A kind of Angelica rhizosphere high-efficiency phosphate solubilizer bacterial agent, it is prepared by following method:
[0026] Take Angelica rhizosphere phosphorus-solubilizing bacteria Bacillus sp.DGP01 (preservation number is CGMCC No.4755) bacterial strain and inoculate in the seed culture medium shake flask, 30 ℃, 200 rev / min seed vibration culture to logarithmic growth phase; Then above-mentioned culture A good strain is inoculated into a 500-liter seed tank equipped with a fermentation medium according to a 10% inoculum amount, and the feeding amount is 400 liters, and it is cultivated to the logarithmic growth phase; There are 5 tons of production tanks with fermentation medium for cultivation, and the feeding amount is 4 tons. The culture liquid obtained from fermentation is directly packed into liquid dosage forms of phosphobacteria inoculum in plastic packaging barrels or packaging bottles.
[0027] Above-mentioned seed culture medium, seed tank and the us...
Embodiment 2
[0030] A kind of Angelica rhizosphere high-efficiency phosphate solubilizer bacterial agent, it is prepared by following method:
[0031] Take Angelica rhizosphere phosphorus-solubilizing bacteria Bacillus sp.DGP01 (preservation number is CGMCC No.4755) bacterial strain and inoculate in the seed culture medium shake flask, 30 ℃, 200 rev / min seed vibration culture to logarithmic growth phase; Then above-mentioned culture A good bacterial classification is inoculated into a 500 liter seed tank equipped with a fermentation medium according to a 10% inoculum amount, and the feeding amount is 450 liters, and it is cultivated to the logarithmic growth phase; There are 5 tons of production tanks with fermentation medium for cultivation, and the feeding amount is 4.5 tons. The culture liquid obtained from fermentation is absorbed by peat and packed into solid dosage forms of phosphate-solubilizing bacteria bacterial agents with packaging bags.
[0032] Above-mentioned seed culture med...
Embodiment 3
[0034] Example 3 Phosphorus Solubilizing Ability Test of Phosphorus Solubilizing Bacteria
[0035] 1. Determination of the phosphorus-solubilizing ability of the strain by liquid culture method
[0036] 30 mL of sterilized seed culture medium (glucose 10 g, (NH 4 ) 2 SO 4 0.5g, KCl0.3g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, KH 2 PO 4 1.0g, distilled water 1000mL, pH 7.0~7.5), insert the activated slant Bacillus sp.DGP01 strain (preservation number is CGMCC No.4755), and shake the flask to culture to the logarithmic growth phase (about 10 8 CFU mL -1 ). Insert phosphorus-dissolving culture solution (250mL triangular bottle with 40mL culture medium) according to 5% inoculum amount respectively, set up the control group that does not inoculate strain at the same time, each treatment repeats 5 times, shake at 32 ℃ after inoculation, 200 rev / min constant temperature After culturing on the bed for 7-10 days, take 10ml of the culture ...
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