A potassium-dissolving strain suitable for low temperature environment and fermentation method for preparing microecological preparation based on the strain

A low-temperature environment and micro-ecological bacterial agent technology, applied in the field of microorganisms, can solve the problems of serious environmental pollution, high production cost, and large amount of ingredients, and achieve the effects of simple production process, high production efficiency, and increased output

Active Publication Date: 2020-07-28
HUNAN AGRICULTURAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, researchers have proposed a variety of methods for the development and utilization of potassium fertilizers produced from insoluble potassium ore resources, which can be summarized into two categories: high-temperature methods and wet methods. High cost, serious environmental pollution and other shortcomings, has not been able to achieve large-scale industrial production

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  • A potassium-dissolving strain suitable for low temperature environment and fermentation method for preparing microecological preparation based on the strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The screening and separation of embodiment 1 potassium solubilizing bacteria

[0026] Several soil samples were collected from the potassium feldspar mining area in the Tibet Autonomous Region, stored in sterile plastic bags, and separated immediately after being sent back by air. Under aseptic operation, weigh 1.0g of soil sample and dissolve it in 9ml of sterile physiological saline, add glass beads, fully shake at 180rpm on a shaker at 28°C for 30min, dilute in gradient, and spread on the prepared separation medium plate superior. Place it upside down in an incubator at 28-30°C for 2-3 days, use an inoculation needle to pick up the colonies with rounded edges, moist, viscous, colorless, transparent and elastic, and further separate and purify them. The purified strains were transferred to a screening medium plate with potassium feldspar as the only potassium source, cultured at 28°C for 4 days, and well-grown, large, smooth, round, transparent, moist, viscous, and e...

Embodiment 2

[0030] The mensuration of the potassium decomposing ability of embodiment 2 bacterial strains

[0031] The strains obtained by screening were inoculated into 100 mL of growth medium, and inoculated into potassium solution assay medium with a 2% inoculum amount. The control group was inoculated with an equal amount of inactivated bacteria solution, and cultured at 28° C. and 160 rpm for 5 days with shaking. Take 10ml of strain culture solution, add 6% H 2 o 2 2ml, digested in a boiling water bath for 1h, centrifuged the above digested solution at 4000rpm for 20min, took the supernatant, and measured K by atomic absorption spectrophotometry. + concentration, and calculate the decomposition rate of the strain on potassium feldspar ore powder.

[0032] The formula for calculating potassium solution rate is as follows:

[0033]

[0034] Among the screened strains, the BX strain has the highest potassium-dissolving ability, and the K in its fermentation broth + The concentrat...

Embodiment 3

[0035] The identification of embodiment 3 potassium solubilizing bacteria BX

[0036] The growth of the strain BX in the only carbon source or the only nitrogen source medium was measured, and the colony morphology was observed to obtain the morphological characteristics of the potassium-solubilizing bacteria BX. The colony of the strain BX on the solid medium was light yellow, round, rough and opaque, about 5 mm in diameter, and protruding in the middle. Microscopically, the bacteria were short rod-shaped and Gram stain negative. Can utilize glucose, starch, sucrose, maltose, lactose, glucomannan, galactose; can decompose glucose to produce pyruvate, decompose tryptophan to form indole, can hydrolyze starch, and have the ability to hydrolyze lecithin. The total DNA of strain BX was extracted by lysis method, and 16s rDNA was amplified for bacterial molecular identification. The amplified product was sequenced by Shanghai Maipu Biotechnology Co., Ltd., and compared with the h...

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Abstract

The invention discloses a potassium-dissolving bacterial strain suitable for low-temperature environment and a fermentation method for preparing microecological preparations based on the bacterial strain. The bacterial strain is identified as Bacillus, and the strain number is BX, which is preserved in the General Microorganism Center of China Microbiological Culture Preservation Management Committee , the deposit number is CGMCC No.14769. The strain can effectively decompose insoluble inorganic minerals such as potassium feldspar and apatite, increase the content of available nutrients in crop soil, and promote the growth and development of crops, and the strain also has a strong potassium-decomposing effect in low temperature environments, and can be used for Soil improvement in high altitude and cold northern regions has good application value in the preparation of bio-organic fertilizers and soil conditioners.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a potassium-dissolving bacterial strain suitable for low-temperature environments and a fermentation method for preparing probiotics based on the bacterial strain. Background technique [0002] Potassium is one of the three basic nutrients necessary for crops, which can promote the growth and development of crops, and improve the disease resistance and stress resistance of crops. Therefore, potassium is processed into a large amount of potassium fertilizer and used in agriculture. Soil is a natural potassium pool, rich in potassium, but more than 95% of potassium exists in silicate minerals such as potassium feldspar or mica in a slow-acting state, which cannot be directly absorbed and utilized by crops, which leads to The soil is both rich in potassium and deficient in potassium. [0003] On the one hand, due to the lack of easily available potassium resourc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05G3/80C05G5/12C05G5/23C05G5/27C09K17/14C12R1/07C09K101/00
CPCC09K17/14C09K2101/00C12N1/20C05F17/20C12N1/205C12R2001/07Y02W30/40
Inventor 周海燕田云肖红仕林元山潘虎白军平达娃次顿王翀管桂萍周辉刘虎虎卢向阳
Owner HUNAN AGRICULTURAL UNIV
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