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Application of antineoplastic polypeptide TT-1 in preparing antineoplastic medicines

An anti-tumor drug, TT-1 technology, applied in the field of biomedicine, can solve the problems of high cost of polypeptide production, high toxicity, and poor stability of polypeptides to proteasomes

Inactive Publication Date: 2015-01-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are three bottlenecks in the development of peptides: high production cost of peptides; high toxicity; poor stability of peptides to proteasomes

Method used

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  • Application of antineoplastic polypeptide TT-1 in preparing antineoplastic medicines
  • Application of antineoplastic polypeptide TT-1 in preparing antineoplastic medicines
  • Application of antineoplastic polypeptide TT-1 in preparing antineoplastic medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 In vitro inhibitory activity of polypeptide TT-1 on human thyroid ductal carcinoma TT cells

[0012] Take TT cells in the logarithmic growth phase, adjust the cell concentration to 5×10 with F12K medium containing 10% FBS 4 cells / ml, 100 μl of cell suspension was added to a 96-well culture plate, and 20 μl / well of different concentrations of TT-1 diluted with culture medium were added (final concentrations were: 0, 1, 2, 4, 8, 16, 32 μg / well, respectively). ml), 37℃, 5%CO 2 After culturing for 48 hours, aspirate the supernatant, add 80 μl of fresh culture medium, then add 20 μl of 5 mg / ml MTT to each well, continue to cultivate for 4 hours, centrifuge, carefully aspirate the supernatant, add 150 μl DMSO to each well, shake gently to make The formazan was completely dissolved, and the absorbance was measured with a microplate reader at a wavelength of 570 nm. The cell viability was calculated according to literature, cell viability (%)=(average absorbance of ...

Embodiment 2

[0014] Example 2 Inhibitory activity of polypeptide TT-1 on human thyroid ductal carcinoma TT cells in vivo

[0015] Human thyroid ductal carcinoma TT cells in the logarithmic growth phase were diluted with normal saline, stained with trypan blue and counted under an inverted microscope with a hemocytometer. The number of viable cells was above 98%, and the cell concentration was adjusted to 4×10 7 / ml, subcutaneously inoculate 0.1ml of cells in the right side of the armpit of nude mice, and wait until the tumor volume of the mice grows to 50mm 3 At that time, they were randomly divided into 4 groups: tumor model group, TT-1 low, medium and high dose treatment groups, 6 rats in each group. TT-1 low, medium and high dose groups were 0.04, 0.2, 1mg / kg, administered once every 5 days, each injection of 50 μl of drug, the tumor model group was injected with the same amount of normal saline each time, and the administration methods were the same. For intratumoral injection. The a...

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Abstract

The invention belongs to the technical field of biomedicine, and in particular relates to immune regulation polypeptide and application thereof. By modifying melittin, original instable amino acid and proline are replaced into positively charged lysine, thus enhancing stability; meanwhile, an active central part at N terminal is retained; antineoplastic polypeptide TT-1 with relatively high activity is obtained through a solid-phase chemical synthesis method. Result of a pharmacodynamic experiment proves that the TT-1 can inhibit growth of human thyroid carcinoma TT and tumor of mice with tumor, and the antineoplastic polypeptide is relatively low in toxicity, showing that the TT-1 can serve as a medicine for antineoplastic therapy.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to an anti-tumor polypeptide and its application. The polypeptide of the invention has anti-tumor activity. Background technique [0002] Antimicrobial peptides are a class of small molecular polypeptides that are induced in organisms to kill or inhibit bacteria or viruses, and have antibacterial, antiviral and antitumor activities. Antimicrobial peptide is a new type of antimicrobial polypeptide, which is positively charged, rich in hydrophobic bases, and can form an amphipathic structure. Compared with normal cells, tumor cells highly express anionic substances such as phosphatidylserine and heparan sulfate, which makes the cell membrane negatively charged, enhances its interaction with antimicrobial peptides, and produces membrane lysis of tumor cells, so that antimicrobial peptides have specific selection for tumor cells sexual effect. Antimicrobial peptides hav...

Claims

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Application Information

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IPC IPC(8): A61K38/08A61P35/00
Inventor 张大奇万兰兰张金男刘晓莉孙辉
Owner JILIN UNIV
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