Nuclear matrix protein 22 chemiluminescent immunodetection reagent kit and preparing method thereof

A chemiluminescence immunoassay and nuclear matrix protein technology, applied in the field of immunoassay medicine, can solve the problems of difficulty in discovering carcinoma in situ or flat carcinoma, limiting the application of bladder cancer diagnosis, time-consuming and laborious, etc., achieving enhanced chemiluminescence intensity and convenient operation. , the effect of prolonging the luminous time

Inactive Publication Date: 2015-01-07
ZHEJIANG ORIENT GENE BIOTECH
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, the diagnosis and postoperative review of bladder cancer mainly rely on cystoscopy and urine cytology examination. Although the former is accurate, it is an invasive examination, which is complicated, time-consuming, laborious, expensive, and brings great pain to the patient. Many patients are resistant to this method, and it is difficult to find carcinoma in situ or flat carcinoma; urine cytology is non-invasive and ha

Method used

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  • Nuclear matrix protein 22 chemiluminescent immunodetection reagent kit and preparing method thereof
  • Nuclear matrix protein 22 chemiluminescent immunodetection reagent kit and preparing method thereof
  • Nuclear matrix protein 22 chemiluminescent immunodetection reagent kit and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1-6

[0036] Examples 1-6 are the preparation of nuclear matrix protein 22 (NMP22) chemiluminescence immunoassay detection kit

Embodiment 1

[0038] 1. Preparation of enzyme-labeled antibody

[0039] Horseradish peroxidase-labeled nuclear matrix protein 22 (NMP22) monoclonal antibody was prepared by the modified sodium periodate method, and the enzyme-labeled antibody was diluted with the enzyme-labeled antibody diluent at a working concentration of 1:6000;

[0040] 1. Preparation of horseradish peroxidase-labeled nuclear matrix protein 22 (NMP22) monoclonal antibody

[0041] The nuclear matrix protein 22 (NMP22) monoclonal antibody used for labeling and horseradish peroxidase were combined by the modified sodium periodate method to complete the enzyme-labeled conjugate labeled with horseradish peroxidase.

[0042] Taking 10mg of nuclear matrix protein 22 (NMP22) monoclonal antibody as an example, the specific steps are as follows:

[0043] (1) Weigh 5mg of HRP and add 1ml of 0.1mol / L sodium acetate or water to dissolve, and mix well;

[0044] (2) Add 0.2ml of newly prepared 0.1mol / L NaIO4 solution to the uppe...

Embodiment 3

[0082] The difference from Example 1 is: the alkaline phosphatase is connected to the nuclear matrix protein 22 monoclonal antibody by the glutaraldehyde method; the enzyme-labeled substance is diluted 1:3000 with the enzyme-labeled antibody diluent; the composition is Tris ( 24g), HCl, (15ml), NaCl (160g), KCl (4g), double distilled water (1000ml), Tris-HCl concentrated washing solution with a pH value of 7.4 and CSPD as the luminescent substrate solution.

[0083]

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Abstract

The invention relates to the field of immunoassay medicine, in particular to a nuclear matrix protein 22 chemiluminescent immunodetection reagent kit and a preparing method thereof. The reagent kit can be used in clinic for the early diagnosis and the post-operation therapeutic effect monitoring of bladder cancer. The reagent kit provided by the invention comprises an NMP22 calibrator, a solid-phase carrier pre-coated by an NMP22 monoclonal antibody, an enzyme marker of the NMP22 monoclonal antibody, a chemiluminescent substrate solution acting with the enzyme and a concentrated washing solution. The reagent kit has the advantage that bladder cancer can be simply, conveniently and noninvasively diagnosed in an early phase.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine. Specifically, the invention provides a nuclear matrix protein 22 (NMP22) chemiluminescence immunoassay detection kit and a preparation method thereof. Background technique [0002] Bladder tumors are the most common tumors in the urinary system, and more than 90% are transitional cell carcinomas, of which more than 80% are non-invasive superficial carcinomas, and 10%-20% develop into invasive bladder carcinomas. Transitional cell carcinoma of the bladder is the most common malignant tumor of the urinary system, mostly occurring between the ages of 50 and 70. This disease has a high mortality rate, and early detection and diagnosis play an important role in treatment and prognosis. Data show that the 5-year survival rate of patients diagnosed in the early stage of the disease is greater than 90%, while the 5-year survival rate of patients diagnosed in the late stage is only about 15%-30%. ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N33/535
CPCG01N33/535G01N33/577
Inventor 张华廖钟财
Owner ZHEJIANG ORIENT GENE BIOTECH
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