A combination drug for treating tumors

A technology of drugs and compounds, applied in the field of combined drugs for the treatment of tumors, can solve the problems of unfavorable industrial production, long processing cycle, cumbersome process, etc., and achieve the effects of significant drug efficacy, good synergy and clear molecular structure.

Inactive Publication Date: 2017-04-12
楼兰花
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the role of arsenic trioxide in the treatment of solid tumors has been confirmed in many theoretical studies, there has been no significant progress in the clinical treatment of solid tumors.
The existing technological process is cumbersome, the treatment cycle is long, and the yield is low, which is not conducive to industrial production
Moreover, in the prior art, only one of the active components is extracted from the main two active components in hematoxylin, or brazilian hematoxylin, or brazilian hematoxylin
If the two components of brazilian hematoxylin and brazilian hematoxylin are not extracted separately, one of the active components will be lost in the two main components, which will not only affect the effect of the drug, but also affect the quality of the drug due to the extraction process and method. and other issues that affect the yield of the extract

Method used

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  • A combination drug for treating tumors
  • A combination drug for treating tumors
  • A combination drug for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Preparation of arsenic compound solution

[0054] Accurately weigh 1000mg of As with a purity of 99.9% 2 O 3 Pour the powder into a flask containing 200ml sterile deionized water, As 2 O 3 As a suspension, after heating and dripping 4mol / L NaOH until the powder is completely dissolved, add 750ml sterile deionized water, then adjust the solution to pH 7.3 with 0.3mol / L NaOH, and make up the volume with sterile deionized water 1000ml arsenic compound solution, the concentration is based on the feeding As 2 O 3 Calculated as 1mg / ml. As prepared 2 O 3 The solution was sterilized by filtration (through a 0.22 μm filter) and stored in a sterile vial.

Embodiment 2

[0055] Example 2: Preparation of Brazilian hematoxylin compounds

[0056] 1) Preparation of Brazilian hematoxylin

[0057] The crushed heartwood of traditional Chinese medicine hematoxylin is used as the raw material, with distilled water or deionized water as the solvent, and the mass ratio of hematoxylin to distilled or deionized water is 6 times, and it is extracted at 95℃ for 1h; extracted 3 times, combined extraction Use a 240-mesh sieve or filter screen to filter, filter with medium or fast qualitative filter paper or place it on a Buchner funnel. Use a vacuum pump to filter the hematoxylin extract to obtain the hematoxylin water extract; place the hematoxylin water extract on a rotating Concentrate in an evaporator or on an electric furnace, and concentrate to 1 / 15 of the total volume of hematoxylin extract to obtain hematoxylin concentrate; leave the concentrated solution overnight to form a crystalline precipitate, separate the upper hematoxylin concentrate and crystalline...

Embodiment 3

[0060] Example 3: The arsenic compound prepared in Example 1 and the aqueous extract of hematoxylin prepared in step 1) of Example 2 (because the components in the aqueous extract are the Brazilian hematoxylin compounds, the aqueous extract of hematoxylin is used for the preparation In vitro experiment) Inducing tumor cell apoptosis effect in vitro experiment.

[0061] 1. Test materials

[0062] 1) Cell line: T lymphoma cell line Jurkat cell was selected from three cell lines of T lymphoma cell line Jurkat cell, B lymphoma cell line Daudi cell, and liver cancer cell HEPG2 for experiment.

[0063] 2) Drug: arsenic compound (the preparation method is: the arsenic compound solution prepared in Example 1, first diluted with deionized water to 80μg / ml, and then diluted with 1640 broth to the desired concentration); Step 1 of Example 2 ) The prepared hematoxylin water extract (dilute with 1640 culture medium to the required concentration).

[0064] 2. Experimental methods and procedures

[...

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Abstract

The invention discloses a combined medicine for treating tumors. The combined medicine is prepared from unit preparations of an arsenic compound solution and a brazilin compound with different specifications, wherein the arsenic compound solution is prepared by the following steps: adding As2O3 powder into aseptic deionized water to obtain a suspension liquid, and dripping a NaOH solution into the suspension liquid until powder is completely dissolved. The brazilin compound consists of brazilin and brazilein. According to the combined medicine, an arsenic compound and a brazilin compound are combined, so that the curative effect of the arsenic compound can be improved, the dosage and corresponding toxic and side effects of the arsenic compound can be controlled, the combined medicine can be used more safely, and the therapeutic spectrum of the arsenic compound can be extended. The combined medicine has an especially excellent synergetic effect in the aspect of T-lymphoma cell line Jurkat cells.

Description

Technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to the application of arsenic compounds and Brazilian hematoxylin compounds in the preparation of combined drugs for treating tumors, and a combined drug for treating tumors. Background technique [0002] Arsenic trioxide (As203) is a traditional Chinese medicine arsenic. In the 1970s, Chinese scholars discovered that it was effective in treating acute promyelocytic leukemia (APL), which attracted wide attention from researchers and clinicians. But different tumor cells affect As 2 O 3 The susceptibility varies greatly. For example, CN01107536.8 relates to a medicine for the treatment of malignant lymphoma and a preparation method thereof, which discloses that Raji cells derived from the human Burkitt lymphoma cell line have an effect on As 2 O 3 It is very sensitive, and Jurkat cells, which are also derived from human T lymphoma cell line, are sensitive to As 2 O 3 No...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/36A61P35/00A61K31/352
CPCA61K31/352A61K33/36A61K2236/15A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 楼兰花
Owner 楼兰花
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