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Method for preparing cordyceps sinensis strain material by utilizing cultivation chamber

A technology for Cordyceps sinensis fungus and Cordyceps sinensis is applied in the field of preparation of Cordyceps sinensis strain materials, and can solve the problems of limited yield of strain materials, low infection activity, restrictions and the like

Active Publication Date: 2015-01-28
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods have problems such as low infection activity, low survival rate after injury, or too long time-consuming, or limited yield of strain materials, which is one of the key problems restricting the industrialization of original ecological Cordyceps sinensis medicinal materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Choose an area with convenient transportation and cheap electricity, and build an alpine plant cultivation room with automatic control of climate conditions with a room unit of 10m×10m. The cultivation room is equipped with 5-layer cultivation racks for placing plant seedling cultivation bottles; the cultivation room is installed conventionally The industrial cold storage uses a central air-conditioning system to adjust the air temperature in the cultivation room; installs spray pipelines and the central air-conditioning system to jointly adjust the air humidity in the cultivation room; installs a daylight lighting system on the cultivation frame to provide light for the cultivated plants; uses activated carbon microfiltration membrane air filtration system, Keep the cultivation room clean and dust-free; install ultraviolet lamps in the cultivation room and disinfect regularly. Before entering the cultivation room, the staff should change the sterilized work clothes, s...

Embodiment 2

[0026] 1. In the Qinghai-Tibet Plateau at an altitude of 4,500 meters, where the railway transportation is convenient, an alpine plant cultivation room with automatic climate control is built with a unit of 20m×20m. The cultivation room is equipped with 10-layer cultivation racks for placing plant seedling cultivation bottles; Install a central air-conditioning ventilation system for conventional industrial cold storage to adjust the air temperature in the cultivation room; install a spray pipeline and a central air-conditioning system to jointly adjust the air humidity in the cultivation room; install a daylight lighting system on the cultivation frame to provide light for the cultivated plants; use activated carbon to filter the air Filtration system to keep the cultivation room dust-free and clean; UV lamps are installed in the cultivation room for regular disinfection. Before entering the cultivation room, the staff change the sterilized work clothes, shoes, hats, etc. in th...

Embodiment 3

[0032] 1. In an industrial area with complete industrial supporting facilities and convenient material transportation, build a unit of 20m x 10m in length, build an alpine plant cultivation room with automatic climate control, and set up a 3-layer sterilized soil cultivation rack in the cultivation room; Install a central air-conditioning ventilation system for conventional industrial cold storage to adjust the air temperature in the cultivation room; install a spray pipeline and a central air-conditioning system to jointly adjust the air humidity in the cultivation room; install a daylight lighting system on the cultivation frame to provide light for the cultivated plants; use activated carbon to filter the air Filtration system to keep the cultivation room dust-free and clean; UV lamps are installed in the cultivation room for regular disinfection. Before entering the cultivation room, the staff change the sterilized work clothes, shoes, hats, etc. in the dressing room.

[00...

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Abstract

The invention relates to a method for preparing a cordyceps sinensis strain material with high infection activity by utilizing a cultivation chamber. According to the method, a psychrophyte tissue culture seedling cultivation chamber of which the climatic conditions can be controlled automatically or manually is established; the climatic factors such as the temperature, the humidity, the illumination and the like of the Qinghai-Tibet Plateau are simulated; psychrophyte tissue culture seedlings are obtained by using a tissue culture rapid propagation and cultivation technology; the prepared cordyceps sinensis resource is sprayed and incubated to the psychrophyte tissue culture seedlings; an adaption environment is created to promote the commensalism of Hirsutella sinensis and psychrophyte tissue during an asexual stage of cordyceps sinensis; the infection rate of the Thitarodes larvae with the cordyceps sinensis material after being subjected to commensalism processing can reach 95.8 to 98.9 percent.

Description

technical field [0001] The invention relates to a method for preparing Cordyceps sinensis strain material by using a cultivation room. Background technique [0002] Cordyceps sinensis Ophiocordyceps sinensis (Berk.) Sung, Sung, Hywel-Jones & Spatafora is an entomoascus fungus that parasitizes the larvae of Lepidoptera Lepidoptera bat moth family Hepialidae hook bat moth genus Thitarodes insects, and its anamorph is Hirsutella sinensis sinensis Liu, Guo, Yu & Zeng. A generation of insects of the genus Thitarodes, the host of Cordyceps sinensis, includes four developmental stages: eggs, larvae, pupae, and adults. The larvae live in underground tunnels and are omnivorous. The young rhizomes of alpine plants. [0003] Cordyceps sinensis is distributed in the high-altitude areas of the Qinghai-Tibet Plateau, its growth range is limited, and its output is also extremely limited. It is a precious nourishing Chinese herbal medicine characteristic of the Qinghai-Tibet Plateau, wit...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 钟欣张古忍刘昕阚绪甜李少松何健敏
Owner SUN YAT SEN UNIV
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