Isaria javanica BE01 for effectively preventing and treating hyphantria cunea and application of isaria javanica

Abstract

The invention discloses isaria javanica for preventing and treating hyphantria cunea,wherein the isaria javanica is classified and named as isaria javanica BE01, which is preserved in China General Microbiological Culture Collection Center in No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing on November 28, 2016 with preservation number of CGMCC No.13192. The isaria javanica BE01 strain provided by the invention is separated from stiff hyphantria cunea larvae. Tests prove that the isaria javanica, under a spore concentration higher than 1.0*10<4> spores / mL, has relatively good virulence on the hyphantria cunea at different ages, and an infection rate exceeds 53.33%, and the infection rate of the isaria javanica, under a spore concentration of 1.0*10<8> spores / mL, exceeds 73.33%; therefore, the isaria javanica is capable of effectively preventing and treating the hyphantria cunea.

Description

technical field

[0001] The invention belongs to the technical field of microbes, and in particular relates to strains of I. javanica and its application in pest control. Background technique

[0002] American white moth (Hyphandria cunea), also known as autumn curtain caterpillar and autumn curtain moth, belongs to Lepidor tera, Arctiidae and Arctiinae, and is an important species in many countries. pests. The worm is native to North America, and spread to Europe and Asia through human activities and delivery vehicles in the late 1940s. The American white moth was first discovered in Dandong, Liaoning Province, my country in 1979, causing serious damage, and then spread to Beijing, Tianjin, Hebei, Shandong, Shanxi and other provinces and cities. At present, due to the damage of this invasive lepidopteran pest, huge losses have been caused in forestry and fruit trees and even in agriculture. The hosts of this pest include almost all forest trees, fruit trees, ornamental pl...

Examples

Embodiment 1

[0022] Example 1 Isolation and identification of I. javanica BE01

[0023] I. javanica BE01 was isolated from the larvae of the American white moth collected in Suzhou, Anhui. The method is as follows: the mycelium is picked from the body of the zombie larvae, inoculated on SD AY medium added with ampicillin and streptomycin sulfate, and cultured at 25° C. for 5 days. Pick the hyphae in the center of the formed colony, inoculate them on the SDAY medium added with ampicillin and streptomycin sulfate for purification and culture, and repeat the purification culture for 3 times. One fungus obtained by screening was numbered as BE01.

[0024] 1) Morphological identification: such as Figure 1-3 As shown, the purified strain was inoculated on the Chapei medium, the colony was irregular, the hyphae were white, the spore layer was purple-gray, and the back was light yellow, and the spore bundles could be seen after prolonged culture. Under Zeiss microscope observation, the hyphae ...

Embodiment 2

[0027] Example 2 I. javanica BE01 controls American white moth

[0028] Preparation of spore suspension: pick the mycelia from the isolated and purified I. javanica BE01 strain and inoculate it on a SDAY plate covered with cellophane, culture it at 25°C for 20 days, scrape the mycelia and spores on the cellophane into 0.1% Tween-80 aqueous solution, fully stirred and oscillated, and filtered to obtain spore mother liquor. Measure the spore concentration with a hemocytometer and adjust the spore concentration to 1.0 × 10 4 pcs / mL, 1.0×10 5 pcs / mL, 1.0×10 6 pcs / mL, 1.0×10 7 pcs / mL, 1.0×10 8 individual / mL.

[0029] Adopt spraying method, spray the prepared spore liquid of each concentration on the body of 3-5 instar larvae, control spray 0.1% Tween-80 solution, inoculate 10 heads at each concentration of each larvae age, and each process repeats 3 times , the inoculated larvae were placed in a 25°C incubator at 12:12 light and dark, and raised, and the specific death condit...

technical field

[0001] The invention belongs to the technical field of microbes, and in particular relates to strains of I. javanica and its application in pest control. Background technique

[0002] American white moth (Hyphandria cunea), also known as autumn curtain caterpillar and autumn curtain moth, belongs to Lepidor tera, Arctiidae and Arctiinae, and is an important species in many countries. pests. The worm is native to North America, and spread to Europe and Asia through human activities and delivery vehicles in the late 1940s. The American white moth was first discovered in Dandong, Liaoning Province, my country in 1979, causing serious damage, and then spread to Beijing, Tianjin, Hebei, Shandong, Shanxi and other provinces and cities. At present, due to the damage of this invasive lepidopteran pest, huge losses have been caused in forestry and fruit trees and even in agriculture. The hosts of this pest include almost all forest trees, fruit trees, ornamental pl...

Examples

Embodiment 1

[0022] Example 1 Isolation and identification of I. javanica BE01

[0023] I. javanica BE01 was isolated from the larvae of the American white moth collected in Suzhou, Anhui. The method is as follows: the mycelium is picked from the body of the zombie larvae, inoculated on SD AY medium added with ampicillin and streptomycin sulfate, and cultured at 25° C. for 5 days. Pick the hyphae in the center of the formed colony, inoculate them on the SDAY medium added with ampicillin and streptomycin sulfate for purification and culture, and repeat the purification culture for 3 times. One fungus obtained by screening was numbered as BE01.

[0024] 1) Morphological identification: such as Figure 1-3 As shown, the purified strain was inoculated on the Chapei medium, the colony was irregular, the hyphae were white, the spore layer was purple-gray, and the back was light yellow, and the spore bundles could be seen after prolonged culture. Under Zeiss microscope observation, the hyphae ...

Embodiment 2

[0027] Example 2 I. javanica BE01 controls American white moth

[0028] Preparation of spore suspension: pick the mycelia from the isolated and purified I. javanica BE01 strain and inoculate it on a SDAY plate covered with cellophane, culture it at 25°C for 20 days, scrape the mycelia and spores on the cellophane into 0.1% Tween-80 aqueous solution, fully stirred and oscillated, and filtered to obtain spore mother liquor. Measure the spore concentration with a hemocytometer and adjust the spore concentration to 1.0 × 10 4 pcs / mL, 1.0×10 5 pcs / mL, 1.0×10 6 pcs / mL, 1.0×10 7 pcs / mL, 1.0×10 8 individual / mL.

[0029] Adopt spraying method, spray the prepared spore liquid of each concentration on the body of 3-5 instar larvae, control spray 0.1% Tween-80 solution, inoculate 10 heads at each concentration of each larvae age, and each process repeats 3 times , the inoculated larvae were placed in a 25°C incubator at 12:12 light and dark, and raised, and the specific death condit...

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