Extraction method of isodon excisoides extracts

A technology of pseudo-flavor tea vegetable and extraction method, which is applied in the field of extraction of pseudo-flavor tea vegetable extract, can solve the problems of stagnant chemical components and the like, and achieve the effects of good social benefits, huge economic benefits and broad market.

Inactive Publication Date: 2015-01-28
河南省医药科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the research on the pseudo-fragrant tea plant is mainly stagnant in the extraction and separation of chemical components and the corresponding pharmacological experiments, and no clinically available compounds with anti-cancer activity have been found from the pseudo-fragrant tea plant

Method used

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  • Extraction method of isodon excisoides extracts
  • Extraction method of isodon excisoides extracts
  • Extraction method of isodon excisoides extracts

Examples

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Effect test

Embodiment 1

[0073] A kind of extracting method of the tea vegetable extract that lacks fragrance, specifically implements according to the following steps: take the original medicinal material (aerial part) of tea vegetable that lacks fragrance naturally, take 1 kg of the original medicinal material after drying in the shade, pulverize it with a pulverizer, and use Soak in 5000ml of anhydrous diethyl ether for 21 days (the amount of diethyl ether is: the diethyl ether should submerge the medicinal materials), filter with fast 101 qualitative filter paper at normal temperature and pressure, and concentrate under reduced pressure at a water temperature of 36°C and a pressure of 0.09MPa Obtain dark green extract extract, which is ether extract 29g.

[0074] Ethyl acetate extraction: Pour the residue after ether extraction into a clean enamel plate and volatilize the ether completely at room temperature until there is no ether smell, then put the residue into a 5L spherical flask, add 3500ml o...

Embodiment 2

[0078]A kind of extracting method of pseudo-fragrant tea vegetable extract, concretely implement according to the following steps:

[0079] Ethyl ether extraction: Take the original medicinal materials (aboveground part) of the pseudo-fragrant tea and vegetables, dry them naturally in the shade, take 1kg of the original medicinal materials, pulverize them with a pulverizer, soak them in 5000ml of anhydrous ether for 21 days, and use a medium-speed 102 qualitative filter paper under normal temperature and pressure Filter and concentrate under reduced pressure at a water temperature of 30°C and a pressure of 0.1 MPa to obtain a dark green extract, which is 30 g of ether extract.

[0080] Ethyl acetate extraction: Pour the residue after ether extraction into a clean enamel plate and volatilize the ether completely at room temperature until there is no ether smell, then put the residue into a 5L spherical flask, add 3500ml of ethyl acetate, soak 20h, heated to reflux for 2.5h, and...

Embodiment 3

[0084] A kind of extracting method of pseudo-fragrant tea vegetable extract, concretely implement according to the following steps:

[0085] Ether extraction: Take the original medicinal material (aboveground part) of the tea plant that lacks fragrance, dry it naturally in the shade, take 1kg of the original medicinal material, pulverize it with a pulverizer, soak it in 5000ml anhydrous ether for 21 days, and filter it with fast 101 qualitative filter paper at normal temperature and pressure. At a water temperature of 40°C and a pressure of 0.07 MPa, concentrate under reduced pressure to obtain a dark green extract, which is 29.5 g of ether extract.

[0086] Ethyl acetate extraction: Pour the residue after ether extraction into a clean enamel plate and volatilize the ether completely at room temperature until there is no ether smell. Then put the residue into a 5L spherical flask, add 3500ml of ethyl acetate, soak 28h, heated to reflux for 3h, then filtered under normal temper...

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Abstract

The invention discloses an extraction method of isodon excisoides extracts. The extraction method comprises extracting by use of diethyl ether, ethyl acetate, ethanol and water orderly to obtain a diethyl ether extract, an ethyl acetate extract, an ethanol extract and a water extract, respectively. The four extracts have no cytotoxic effect on normal cells, and thus play an important promoting role in the drug therapy of cancers, and consequently, good social benefits can be produced.

Description

technical field [0001] The invention belongs to the technical field of food and medicine, and in particular relates to a method for extracting tea and vegetable extracts lacking in fragrance. Background technique [0002] The pseudo-fragrant tea dish is a dicotyledonous plant, Lamiformes, Lamiaceae perennial herb, also known as wild perilla; the rhizome is horizontal, woody, slightly thickened or lumpy, up to 2 cm thick, and densely grown on it. Fibrous roots. Stems erect, numerous, (0.3-)0.5-1(-1.5) m tall, quadrangular, with four grooves, lower part glabrous, upper part pubescent, sometimes branched upper part. Stems and leaves opposite, broadly elliptic or ovate or round ovate, apex sharply caudate, base broadly wedge-shaped or truncate, tapering down suddenly, edges with irregular serrated teeth, firm papery, dark above Green, puberulent along the veins, sparsely strigose and small bristles on the rest, light green below, only sparsely pubescent along the veins, glabro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P35/00
CPCA61K36/53A61K2236/15A61K2236/17A61K2236/33A61K2236/331A61K2236/35A61K2236/39A61K2236/51A61K2236/53
Inventor 陈慧平马方邹敏王瑞娟赵志鸿张燕张壮丽张丽果王桂芳李继成郭威
Owner 河南省医药科学研究院
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