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Injection preparation before pancreas islet extraction by piglet pancreas

An injection preparation and pig technology, applied in the field of islet extraction from newborn pigs, can solve the problems affecting the number and activity of islets and the effect of islet transplantation, and achieve the effects of reducing the loss of islet cells, improving the result of isolation, and reducing apoptosis

Active Publication Date: 2015-02-04
HUNAN XENO LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In other words, the rationality of the procedures for preparing islet cells directly affects the number and activity of the obtained islets, which ultimately affects the effect of islet transplantation

Method used

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  • Injection preparation before pancreas islet extraction by piglet pancreas
  • Injection preparation before pancreas islet extraction by piglet pancreas
  • Injection preparation before pancreas islet extraction by piglet pancreas

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1, injecting the injection preparation of the present invention to carry out the method for extracting islets from neonatal pig pancreas

[0036] 1. Inject octreotide and low-molecular-weight heparin sodium into newborn pigs half an hour to one hour before taking the pancreas, and the injection doses are 1-3μg / kg body weight and 5-15IU / kg body weight respectively;

[0037] 2. Neonatal pigs were anesthetized by intramuscular injection of sodium pentobarbital;

[0038] 3. The newborn pigs were washed once in water containing detergent, water containing 84 disinfectant and clear water, and the water temperature was controlled at about 37°C;

[0039] 4. Fix the newborn pig in a supine position in a sterile tray covered with a middle sheet, and spray complex iodine solution on the operation area of ​​the newborn pig;

[0040] 5. Place the tray on the sterile operating table and spread the hole towel to expose the abdominal operation area;

[0041] 6. Sterile surgic...

Embodiment 2

[0047] Example 2, Digestion of Neonatal Porcine Pancreas:

[0048] Take out the pancreatic tissue placed in cold D-Hank’s solution, weigh it, and cut it into about 0.5-1mm 3 Rinse until the liquid is clear, centrifuge to remove the supernatant, add 0.25mg / ml type V collagenase, place in a water bath at 37°C for 5-12 minutes, shake and digest, and quickly use pre-cooled pig serum after digestion The D-Hank's solution was used to stop the digestion, and the solution was centrifuged and washed 2-3 times at 1000 rpm×1 min to obtain the islet cell suspension.

Embodiment 3

[0049] Embodiment 3, the cultivation of newborn pig islet cells:

[0050] Add the islet cell suspension to the culture dish with complete medium and place it at 37°C, 5% CO 2 and 95% air incubator for culture, the culture medium and the culture dish were replaced on the first day after the islet cells were isolated, and changed every other day thereafter.

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Abstract

The invention discloses an injection preparation before pancreas islet extraction by piglet pancreas. The injection preparation comprises octreotide and low molecular heparin sodium, the injection amount of octreotide is 1-3mum / kg; and the injection amount of low molecular heparin sodium is 5-15IU / kg. The operation is simple and easy to perform, pancreatin secretion can be greatly reduced, pancreas ischemic time is shortened, ischemic damage is reduced, pancreas islet loss is finally reduced, islet yield can be effectively increased, activity can be protected, the injection preparation provides available resource for preparing and transplanting provide, and has wide clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of newborn pig islet extraction, and in particular relates to an injection preparation for newborn pig pancreas before islet extraction. Background technique [0002] Diabetes is a global high-occurrence metabolic disease that seriously threatens human health and life, and its prevalence is on the rise worldwide. According to the statistics of the International Diabetes Federation in November 2012, the current number of diabetic patients in the world is about It is 366 million and is expected to reach 552 million by 2030; the data also shows that the prevalence of diabetes in China has nearly tripled in the past 10 years. In 2010, the total number of diabetic patients in China has exceeded 90 million. It has become the world's number one diabetic country. Among them, type 1 diabetes patients still need insulin therapy for life, and about 30% of type 2 diabetes patients with a course of more than 20 years ar...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 王维易受南马小倩杨策军
Owner HUNAN XENO LIFE SCI
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