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Method for extracting saikosaponin d from Bupleurum

A technology for saikosaponin and saikosaponin, which is applied in the field of extracting high-content saikosaponin D, can solve problems such as low yield, and achieve the effects of improving extraction rate and efficiency

Inactive Publication Date: 2016-06-08
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for extracting saikosaponin D from Bupleurum in order to solve the technical problem of the low yield of the existing saikosaponin D extraction method

Method used

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Effect test

specific Embodiment approach 1

[0021] Specific embodiment one: the method for extracting saikosaponin D from Bupleuri in this embodiment is as follows:

[0022] Step 1: Crush Bupleurum to 40-60 mesh, take 100g of Bupleurum powder, add 5-8 times the mass of Bupleurum and ethanol with a mass concentration of 75%-90%, then add 0.5-3.3% of the total weight of ethanol and Bupleurum % sodium octyl succinate sulfonate to obtain a mixture;

[0023] Step 2: ultrasonically soak the mixture for 20-40min under the condition of ultrasonic frequency of 40KHz, ultrasonic power of 100-200W, and temperature of 30-50°C, and then heat in a microwave reactor for 20-30 minutes with microwave power of 260-520W, the frequency is 2450MHz, stand still and cool down;

[0024] Step 3: repeat step 2 once, filter, collect filtrate and filter residue;

[0025] Step 4: Add 200-400 g of ethanol with a mass concentration of 80% to the filter residue obtained in Step 3, and then add 1.0-3.0 g of sodium octyl succinate sulfonate;

[0026]...

specific Embodiment approach 2

[0030] Embodiment 2: This embodiment differs from Embodiment 1 in that in step 2, the mixture is ultrasonically soaked at a temperature of 42-49°C. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0031] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that in Step 2, the mixture is ultrasonically soaked at a temperature of 43-48°C. Others are the same as those in the first or second embodiment.

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Abstract

The invention provides a method for extracting saikosaponin D from radix bupleuri, belongs to the technical field of traditional Chinese medicine preparations and specifically relates to a method for extracting high-content saikosaponin D. The method is used for solving the technical problem of low yield of the existing saikosaponin D extraction method. The method for extracting the saikosaponin D from the radix bupleuri comprises the following steps: grinding the radix bupleuri, adding ethanol and sodium octylsulfosuccinate, and performing ultrasonic processing, microwave heating, standing and cooling; repeating the ultrasonic and microwave operations, filtering, and collecting the filtrate and the filter residue; adding ethanol to the filter residue and adding the sodium octylsulfosuccinate again; and performing ultrasonic processing and microwave heating, blending the filtrates, next, boiling off the solvent, adding water for dispersing, stirring, then placing for 2 hours, performing suction filtration, eluting the filtrate, collecting the eluate, and finally, drying the eluate to obtain the saikosaponin powder. The content of the saikosaponin D in the radix bupleuri extract obtained by use of the method is within the range of about 30-40%, and the amount of the saikosaponin D in the radix bupleuri extract is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and in particular relates to a method for extracting high-content saikosaponin D. Background technique [0002] Bupleurum bupleurum is a traditional Chinese medicine. It is cool in nature and bitter in taste. Anti-ulcer and other pharmacological effects have long been valued by the medical circles at home and abroad. Bupleurum is one of the most commonly used traditional Chinese medicines for treating liver diseases, especially saikosaponin D, which is one of the effective monomer components isolated and extracted from the traditional Chinese medicine Bupleurum. pharmacological effects. In recent years, studies have shown that saikosaponin D has a wide range of anti-cancer effects in multiple aspects and mechanisms in anti-hepatic cancer and other aspects. Traditional saikosaponin D extraction methods mainly include the following: [0003] 1. Extract with met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00
CPCC07J63/008
Inventor 赵雪莹李冀
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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