PVAX-PTX3 eukaryotic expression vector, constructing method and antibacterial application of PTX protein expressed by eukaryotic expression vector in streptococcus suis 2

Abstract

The invention belongs to the field of research of a streptococcus suis prevention and control technology, and provides a pVAX-PTX3 eukaryotic expression vector, a constructing method and an antibacterial application of PTX protein expressed by the eukaryotic expression vector in streptococcus suis 2. The gene ptx3 sequence of the PTX3 protein in the recombinant eukaryotic expression vector pVAX-PTX3 is as shown in SEQ ID NO. 1, and the PTX3 protein can be stably expressed in a tissue body. The sequence of the PTX3 is as shown in SEQ ID NO. 2, and the PTX3 protein can be used for preparing a biological preparation for preventing or treating streptococcus suis disease caused by streptococcus suis 2. A pVAX-PTX3 recombinant plasmid is strong in stability and can be stably and effectively expressed in the tissue body, and the PTX3 generated from expression has an antibacterial effect on the streptococcus suis 2; upon in vitro tests, in vivo tests on BALB / c mice and piglets verify that the PTX3 protein expressed by the pVAX-PTX3 has an antibacterial effect on the streptococcus suis 2; and by virtue of the pVAX-PTX3, a novel method for the clinical prevention and treatment of the streptococcus suis disease is established.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of suis streptococcal disease, and relates to a pVAX-PTX3 eukaryotic expression vector, a construction method and the antibacterial application of the expressed PTX3 protein to Streptococcus suis type 2. Background technique [0002] Pentraxins are a multifunctional protein family, including short pentraxins and long pentraxins. PTX3 protein is the first long pentraxin identified [1，2] , which belong to the same class of soluble recognition receptors (PRMs) as the classic short pentraxin-C-reactive protein and the serum amyloid p-complex [3，4] . PTX3 is mainly derived from myeloid dendritic cells, but a variety of cells can express PTX3, including fibroblasts, vascular endothelial cells, monocytes, macrophages, smooth muscle cells, renal epithelial cells, synoviocytes, cartilage cells, adipocytes, and alveolar epithelial cells. [0003] The first researched function of PTX3 is t...

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