Temperature control circulation device and gel electrophoresis system comprising device

Abstract

The invention relates to a temperature control circulation device. The device comprises a water pump, a water inlet pipe, a water outlet pipe, a transformer, an alternating-current relay, a water temperature control switch, a water pump switch, a water temperature controlling switch, a water level controlling switch, a water flow sensing switch, a water pipe temperature control switch, a kernel protection switch, an overheating protection switch, a heating power supply indicator lamp and a heating coil. The invention also relates to a standard gel electrophoresis system using a safe temperature controlled device; the system comprises an electrophoresis box and the temperature control circulation device; a plurality of clamping slots are formed in a bottom plate in the electrophoresis box; a gel plate bracket is fixed in the electrophoresis box by the clamping slots; a plurality of wire slots are formed in the top of the electrophoresis box; electrophoresis wires penetrate in the wire slots; a cover plate is arranged at the top of the electrophoresis box; the temperature control circulation device is arranged on one side of the electrophoresis box. The temperature control circulation device and the gel electrophoresis system comprising the device can be used for implementing the consistency of electrophoresis conditions of the gel electrophoresis and ensuring the proper temperature of electrophoresis liquid.

Description

technical field

[0001] The invention relates to the field of equipment control, in particular to biological technology, in particular to a temperature-controlled circulation device and a gel electrophoresis system comprising the device. Background technique

[0002] Nucleic acid mutation detection technology (DGGE) is an electrophoretic detection technology commonly used in molecular biology. DGGE is an electrophoretic technology that separates DNA with different sequences through the difference in the concentration gradient of denaturant in polyacrylamide gel to achieve the purpose of detection. . The principle of this method is that according to the melting characteristics of DNA, the denaturing agent concentration required for the denaturation of the DNA double helix with different base compositions is different. The corresponding DNA is melted and denatured, resulting in a greatly reduced electrophoretic migration rate and stagnation on the gel. Different DNA molecules...

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