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Bilirubin encephalopathy model production method employing jugular vein catheter

A bilirubin and jugular vein technology, applied in the research field of bilirubin encephalopathy, can solve the problems of low success rate of modeling, unstable high bilirubin level, etc., and achieve the effect of improving the success rate

Inactive Publication Date: 2015-06-03
WENZHOU MEDICAL UNIV
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Problems solved by technology

[0003] At present, some progress has been made in imaging research on hyperbilirubinemia brain injury, but there is a shortcoming that domestic animal models of bilirubin encephalopathy are basically established by intraperitoneal injection of bilirubin solution, but the serum hyperbilirubin The hormone level is unstable, and the success rate of modeling is extremely low, so it is urgent to explore a stable method for rat BE modeling

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  • Bilirubin encephalopathy model production method employing jugular vein catheter
  • Bilirubin encephalopathy model production method employing jugular vein catheter
  • Bilirubin encephalopathy model production method employing jugular vein catheter

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preparation example Construction

[0041] Brain tissue extraction and preparation of serum samples;

[0042] Obtain data on bilirubin concentrations in serum and brain tissue;

[0043] Application of SPSS13.0 software for statistical analysis.

[0044] As an optimization scheme of the embodiments of the present invention, the preparation of bilirubin solution:

[0045] Preparation of bilirubin solution (preparation before use): Weigh 20mg of crystalline bilirubin in the dark and dissolve it in 1ml of 0.5M NaOH solution, add 9ml of double distilled water, and adjust the pH to 8.5 with 0.5M HCL to prepare bilirubin Solution, the concentration is 3.42mmo / L (2mg / ml).

[0046] As an optimization scheme of the embodiment of the present invention, animal modeling: 7-day-old neonatal rats were randomly divided into: control group and model group;

[0047] The limbs of the mouse were fixed on the operating table with medical tape, and the head was fixed with a rubber band. Extract 2% lidocaine hydrochloride injectio...

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Abstract

The invention discloses a bilirubin encephalopathy model production method employing a jugular vein catheter. The bilirubin encephalopathy model production method includes preparing bilirubin solution and performing animal moulding; 30 minutes after the animal moudling, processing animal and collecting samples; taking brain tissue of the animal and observing morphological changes of the brain tissue; extracting the brain tissue and preparing serum samples; obtaining data of serum and bilirubin concentration of the brain tissue; applying software SPSS13.0 to do statistic analysis. By the use of the bilirubin encephalopathy model production method, moulding success rate and stability is greatly increased, and the model is wide in promotion range and supports technical guarantee for research of the bilirubin encephalopathy.

Description

technical field [0001] The invention belongs to the technical field of research on bilirubin encephalopathy, in particular to a method for making a model of bilirubin encephalopathy by jugular vein intubation Background technique [0002] Hyperbilirubinemia is a common neonatal clinical disease, severe cases can lead to fatal bilirubin encephalopathy or severe neurological sequelae. It can endanger the lives of children, and survivors often have severe neurological sequelae such as athetosis, eye movement disorders, hearing impairment, and mental retardation. So far, for bilirubin brain injury, a disease that seriously endangers the health of newborns, there is no specific treatment at home and abroad, and prevention is still the priority. In clinical practice, due to reasons such as technology and medical ethics, neurotransmitter research and histopathological examination of neonatal hyperbilirubin brain injury are not suitable to be obtained in vivo, and the mechanism of ...

Claims

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Application Information

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IPC IPC(8): A61D7/00A61D1/00G01N1/28
CPCA61D1/00A61D7/00G01N1/28
Inventor 高红昌赵良才严志汉白光辉胡文弋程小杰
Owner WENZHOU MEDICAL UNIV
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